Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ASCO Annual Meeting 2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

2024 ASCO Annual Meeting

Keep up to date with all the top stories and latest evidence with our hub page, including official congress videos and expert takeaways.
ADVANCED SEARCH
Log in
Top
Chinese Medicine
Published in: Chinese Medicine 1/2017

Open Access 01-12-2017 | Research

Chemical characterization of wound ointment (WO) and its effects on fracture repair: a rabbit model

Authors: Zhixue Ou, Qi Cheng, Yueping Chen, Tao Chen, Xiangbin Rong, Feipan Long, Xiaoyun Zhang, Qinghua Liang, Zhe Feng

Published in: Chinese Medicine | Issue 1/2017

Login to get access

Abstract

Background

Wound ointment (WO), a kind of Chinese medicine, can significantly promote fracture healing. The study aimed at analyzing the chemical composition and the effects of WO on fracture of rabbits and tried to explore the corresponding molecular mechanism in cytokine.

Methods

The qualitative and quantitative analysis of WO was conducted by liquid chromatography-mass spectrometry (LC–MS). Fifty-four Zealand mature male rabbits were randomly divided into 3 groups: Control group, Yunnan Baiyao (YB) group and WO group. All the rabbits suffered a fracture of right radius and were then stabilized with an external fixator. Treated with different methods, fracture healing was observed. The bone specimens were subjected to radiograph, immunohistochemistry (IHC) analysis, hematoxylin–eosin staining (HE), western blot and enzyme linked immunosorbent assay (ELISA).

Results

A total of 12 active compositions were detected by LC–MS. Radiographs showed a considerably better bone healing and remodeling of the fracture in WO group. HE experiments showed that a large number of osteoclasts appeared in the early stage when treated with WO. In immunohistochemistry (IHC), western blot and ELISA test, significant increases in vascular endothelial growth factor (VEGF) expression were observed in WO group compared with other two groups.

Conclusions

Wound ointment contained active compositions which efficiently promoted fracture healing through increasing the expression of VEGF.
Trial Registration Not applicable
Appendix
Available only for authorised users
Literature
1.
Dombrowski S, Jacob L, Hadji P, Kostev K. Oral contraceptive use and fracture risk-a retrospective study of 12,970 women in the UK. Osteoporos Int. 2017;28(8):2349–55.CrossRefPubMed
2.
Cunningham TD, Martin BC, DeShields SC, Romero CC. The impact of osteoporotic fractures compared with other health conditions in older adults living in Virginia, United States. Osteoporos Int. 2016;27:2979–88.CrossRefPubMed
3.
Benetou V, Orfanos P, Feskanich D, Michaelsson K, Pettersson-Kymmer U, Eriksson S, et al. Fruit and vegetable intake and hip fracture incidence in older men and women: the CHANCES project. J Bone Miner Res. 2016;31:1743–52.CrossRefPubMed
4.
Ogilvie CM, Lu C, Marcucio R, Lee M, Thompson Z, Hu D, et al. Vascular endothelial growth factor improves bone repair in a murine nonunion model. Iowa Orthop J. 2012;32:90–4.PubMedPubMedCentral
5.
Karres J, Heesakkers NA, Ultee JM, Vrouenraets BC. Predicting 30-day mortality following hip fracture surgery: evaluation of six risk prediction models. Injury. 2015;46:371–7.CrossRefPubMed
6.
Du B, Liu W, Deng Y, Li S, Liu X, Gao Y, et al. Angiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhVEGF165 in critical-size alveolar bone defects in vivo. Int J Nanomed. 2015;10:2555–65.
7.
Grassmann JP, Schneppendahl J, Sager M, Hakimi AR, Herten M, Loegters TT, et al. The effect of bone marrow concentrate and hyperbaric oxygen therapy on bone repair. J Mater Sci Mater Med. 2015;26:5331.CrossRefPubMed
8.
Katagiri W, Kawai T, Osugi M, Sugimura-Wakayama Y, Sakaguchi K, Kojima T, et al. Angiogenesis in newly regenerated bone by secretomes of human mesenchymal stem cells. Maxillofac Plast Reconstr Surg. 2017;39:8.CrossRefPubMedPubMedCentral
9.
Sukul M, Nguyen TB, Min YK, Lee SY, Lee BT. Effect of local sustainable release of BMP2-VEGF from nano-cellulose loaded in sponge biphasic calcium phosphate on bone regeneration. Tissue Eng Part A. 2015;21:1822–36.CrossRefPubMedPubMedCentral
10.
Frederick J, Boysen S, Wagg C, Chalhoub S. The effects of oral administration of Yunnan Baiyao on blood coagulation in beagle dogs as measured by kaolin-activated thromboelastography and buccal mucosal bleeding times. Can J Vet Res. 2017;81:41–5.PubMedPubMedCentral
11.
Murphy LA, Panek CM, Bianco D, Nakamura RK. Use of Yunnan Baiyao and epsilon aminocaproic acid in dogs with right atrial masses and pericardial effusion. J Vet Emerg Crit Care (San Antonio). 2017;27:121–6.CrossRef
12.
Li R, Alex P, Ye M, Zhang T, Liu L, Li X. An old herbal medicine with a potentially new therapeutic application in inflammatory bowel disease. Int J Clin Exp Med. 2011;4:309–19.PubMedPubMedCentral
13.
Zhou J, Ni M, Liu X, Ren Z, Zheng Z. Curcumol promotes vascular endothelial growth factor (VEGF)-mediated diabetic wound healing in streptozotocin-induced hyperglycemic rats. Med Sci Monit. 2017;23:555–62.CrossRefPubMedPubMedCentral
14.
Tang QL, Han SS, Feng J, Di JQ, Qin WX, Fu J, et al. Moist exposed burn ointment promotes cutaneous excisional wound healing in rats involving VEGF and bFGF. Mol Med Rep. 2014;9:1277–82.CrossRefPubMed
15.
Ho TJ, Jiang SJ, Lin GH, Li TS, Yiin LM, Yang JS, et al. The in vitro and in vivo wound healing properties of the Chinese herbal medicine “Jinchuang Ointment”. Evid Based Complement Alternat Med. 2016;2016:1654056.PubMedPubMedCentral
16.
Arti H, Khorami M, Ebrahimi-Nejad V. Comparison of negative pressure wound therapy (NPWT) & conventional wound dressings in the open fracture wounds. Pak J Med Sci. 2016;32:65–9.PubMedPubMedCentral
17.
Yang GE, Duan X, Lin D, Li T, Luo D, Wang L, et al. Rapamycin-induced autophagy activity promotes bone fracture healing in rats. Exp Ther Med. 2015;10:1327–33.CrossRefPubMedPubMedCentral
18.
Murphy CM, Schindeler A, Cantrill LC, Mikulec K, Peacock L, Little DG. PTH(1-34) treatment increases bisphosphonate turnover in fracture repair in rats. J Bone Miner Res. 2015;30:1022–9.CrossRefPubMed
19.
Ke K, Li Q, Yang X, Xie Z, Wang Y, Shi J, et al. Asperosaponin VI promotes bone marrow stromal cell osteogenic differentiation through the PI3K/AKT signaling pathway in an osteoporosis model. Sci Rep. 2016;6:35233.CrossRefPubMedPubMedCentral
20.
Berendsen AD, Pinnow EL, Maeda A, Brown AC, McCartney-Francis N, Kram V, et al. Biglycan modulates angiogenesis and bone formation during fracture healing. Matrix Biol. 2014;35:223–31.CrossRefPubMed
21.
Ozturk BY, Inci I, Egri S, Ozturk AM, Yetkin H, Goktas G, et al. The treatment of segmental bone defects in rabbit tibiae with vascular endothelial growth factor (VEGF)-loaded gelatin/hydroxyapatite “cryogel” scaffold. Eur J Orthop Surg Traumatol. 2013;23:767–74.CrossRefPubMed
22.
Song N, Zhao Z, Ma X, Sun X, Ma J, Li F, et al. Naringin promotes fracture healing through stimulation of angiogenesis by regulating the VEGF/VEGFR-2 signaling pathway in osteoporotic rats. Chem Biol Interact. 2017;261:11–7.CrossRefPubMed
23.
24.
Stegen S, van Gastel N, Carmeliet G. Bringing new life to damaged bone: the importance of angiogenesis in bone repair and regeneration. Bone. 2015;70:19–27.CrossRefPubMed
25.
Issa JP, Ingraci de Lucia C, Dos Santos Kotake BG, Goncalves Gonzaga M, Tocchini de Figueiredo FA, Mizusaki Iyomasa D, et al. The effect of simvastatin treatment on bone repair of femoral fracture in animal model. Growth Factors. 2015;33:139–48.CrossRefPubMed
26.
Lee JY, Son SJ, Son JS, Kang SS, Choi SH. Bone-healing capacity of PCL/PLGA/Duck beak scaffold in critical bone defects in a rabbit model. Biomed Res Int. 2016;2016:2136215.PubMedPubMedCentral
27.
De Kock T, Boone MA, De Schryver T, Van Stappen J, Derluyn H, Masschaele B, et al. A pore-scale study of fracture dynamics in rock using X-ray micro-CT under ambient freeze-thaw cycling. Environ Sci Technol. 2015;49:2867–74.CrossRefPubMed
Metadata
Title
Chemical characterization of wound ointment (WO) and its effects on fracture repair: a rabbit model
Authors
Zhixue Ou
Qi Cheng
Yueping Chen
Tao Chen
Xiangbin Rong
Feipan Long
Xiaoyun Zhang
Qinghua Liang
Zhe Feng
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Chinese Medicine / Issue 1/2017
Electronic ISSN: 1749-8546
DOI
https://doi.org/10.1186/s13020-017-0152-y

Other articles of this Issue 1/2017

Chinese Medicine 1/2017 Go to the issue

Research

A network pharmacology-based strategy deciphers the underlying molecular mechanisms of Qixuehe Capsule in the treatment of menstrual disorders

Research

Electroacupuncture for the prevention of postoperative gastrointestinal dysfunction in participants undergoing vascular laparotomy under general anesthesia: a randomized controlled trial

Research

Relationship between Chinese medicine dietary patterns and the incidence of breast cancer in Chinese women in Hong Kong: a retrospective cross-sectional survey

Review

Cupping therapy versus acupuncture for pain-related conditions: a systematic review of randomized controlled trials and trial sequential analysis

Research

An investigation of fungal contamination on the surface of medicinal herbs in China

Research

Metabolic regulations of a decoction of Hedyotis diffusa in acute liver injury of mouse models