Top

Clinical Oral Investigations

Published in:

01-04-2015 | Original Article

Characterization of un-hydrated and hydrated BioAggregate™ and MTA Angelus™

Authors: J. Camilleri, F. Sorrentino, D. Damidot

Published in: Clinical Oral Investigations | Issue 3/2015

Abstract

Objectives

BioAggregate™ is a novel material introduced for use as a root-end filling material. It is tricalcium silicate-based, free of aluminium and uses tantalum oxide as radiopacifier. BioAggregate contains additives to enhance the material performance. The purpose of this research was to characterize the un-hydrated and hydrated forms of BioAggregate using a combination of techniques, verify whether the additives if present affect the properties of the set material and compare these properties to those of MTA Angelus™.

Methods

Un-hydrated and hydrated BioAggregate and MTA Angelus were assessed. Un-hydrated cement was tested for chemical composition, specific surface area, mineralogy and kinetics of hydration. The set material was investigated for mineralogy, microstructure and bioactivity. Scanning electron microscopy, X-ray energy dispersive spectroscopic analysis, X-ray fluorescence spectroscopy, X-ray diffraction and isothermal calorimetry were employed. The specific surface area was investigated using a gas adsorption method with nitrogen as the probe.

Results

BioAggregate was composed of tricalcium silicate, tantalum oxide, calcium phosphate and silicon dioxide and was free of aluminium. On hydration, the tricalcium silicate produced calcium silicate hydrate and calcium hydroxide. The former was deposited around the cement grains, while the latter reacted with the silicon dioxide to form additional calcium silicate hydrate. This resulted in reduction of calcium hydroxide in the aged cement. MTA Angelus reacted in a similar fashion; however, since it contained no additives, the calcium hydroxide was still present in the aged cement. Bioactivity was demonstrated by deposition of hydroxyapatite. BioAggregate exhibited a high specific surface area. Nevertheless, the reactivity determined by isothermal calorimetry appeared to be slow compared to MTA Angelus. The tantalum oxide as opposed to bismuth oxide was inert, and tantalum was not leached in solution. BioAggregate exhibited high calcium ion release early, which was maintained over the 28-day period as opposed to MTA Angelus, which demonstrated low early calcium ion release which increased as the material aged.

Conclusions

The mineralogical composition of BioAggregate was different to MTA Angelus. As opposed to MTA Angelus, BioAggregate did not contain aluminium and contained additives such as calcium phosphate and silicon dioxide. As a consequence, BioAggregate reacted more slowly and formation of calcium hydroxide and leaching of calcium ions in solution were not evident as the material aged. The additives in BioAggregate modify the kinetics and the end products of hydration.

Clinical significance

Although newer generation tricalcium silicate-based materials contain similar constituents to MTA, they do not undergo the same setting reactions, and thus, their clinical performance will not be comparable to that of MTA.

Veriodent web page: http://www.veriodent.com/pb/wp_d66c3664/wp_d66c3664.html. Accessed 30 Sept 11

Park JW, Hong SH, Kim JH, Lee SJ, Shin SJ (2010) X-Ray diffraction analysis of white ProRoot MTA and Diadent BioAggregate. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 109:155–158CrossRefPubMed

Grech L, Mallia B, Camilleri J (2013) Investigation of the physical properties of tricalcium silicate cement-based root-end filling materials. Dent Mater 29:e20–e28CrossRefPubMed

Grech L, Mallia B, Camilleri J (2013) Characterization of set IRM, Biodentine, Bioaggregate and a prototype calcium silicate cement for use as root-end filling materials. Int Endod J 46:632–641CrossRefPubMed

Shokouhinejad N, Nekoofar MH, Razmi H, Sajadi S, Davies TE, Saghiri MA, Gorjestani H, Dummer PM (2012) Bioactivity of EndoSequence root repair material and bioaggregate. Int Endod J 45:1127–1134CrossRefPubMed

Yan P, Yuan Z, Jiang H, Peng B, Bian Z (2010) Effect of bioaggregate on differentiation of human periodontal ligament fibroblasts. Int Endod J 43:1116–1121CrossRefPubMed

Mukhtar-Fayyad D (2011) Cytocompatibility of new bioceramic-based materials on human fibroblast cells (MRC-5). Oral Surg Oral Med Oral Pathol Oral Radiol Endod 112:e137–e142CrossRefPubMed

Yuan Z, Peng B, Jiang H, Bian Z, Yan P (2010) Effect of bioaggregate on mineral-associated gene expression in osteoblast cells. J Endod 36:1145–1148CrossRefPubMed

De-Deus G, Canabarro A, Alves G, Linhares A, Senne MI, Granjeiro JM (2009) Optimal cytocompatibility of a bioceramic nanoparticulate cement in primary human mesenchymal cells. J Endod 35:1387–1390CrossRefPubMed

10.

Dohaithem A, Al-Nasser A, Al-Badah A, Al-Nazhan S, Al-Maflehi N (2011) An in vitro evaluation of antifungal activity of bioaggregate. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 112:e27–e30CrossRefPubMed

11.

Zhang H, Pappen FG, Haapasalo M (2009) Dentin enhances the antibacterial effect of mineral trioxide aggregate and bioaggregate. J Endod 35:221–224CrossRefPubMed

12.

De-Deus G, de Souza MC, Sergio Fidel RA, Fidel SR, de Campos RC, Luna A (2009) Negligible expression of arsenic in some commercially available brands of Portland cement and mineral trioxide aggregate. J Endod 35:887–890CrossRefPubMed

13.

Duarte MA, De Oliveira Demarchi AC, Yamashita JC, Kuga MC, De Campos Fraga S (2005) Arsenic release provided by MTA and Portland cement. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 99:648–650CrossRefPubMed

14.

Monteiro Bramante C, Demarchi AC, de Moraes IG, Bernadineli N, Garcia RB, Spångberg LS, Duarte MA (2008) Presence of Arsenic in different types of MTA and white and gray Portland cement. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 106:909–913CrossRefPubMed

15.

Schembri M, Peplow G, Camilleri J (2010) Analyses of heavy metals in mineral trioxide aggregate and portland cement. J Endod 36:1210–1216CrossRefPubMed

16.

Camilleri J (2008) Characterization of hydration products of mineral trioxide aggregate. Int Endod J 41:408–417CrossRefPubMed

17.

Camilleri (2014) The color stability of white mineral trioxide aggregate in contact with sodium hypochlorite solution. J Endod 40:436–440CrossRefPubMed

18.

Camilleri J, Kralj P, Veber M, Sinagra E (2012) Characterization and analyses of acid extractable and leached trace elements in dental cements. Int Endod J 45:737–743CrossRefPubMed

19.

Camilleri J, Formosa L, Damidot D (2013) The setting characteristics of MTA Plus in different environmental conditions. Int Endod J 46:831–840CrossRefPubMed

20.

Camilleri J, Sorrentino F, Damidot D (2013) Investigation of the hydration and bioactivity of radiopacified tricalcium silicate cement, Biodentine and MTA Angelus. Dent Mater 29:580–593CrossRefPubMed

21.

Reyes-Carmona JF, Felippe MS, Felippe WT (2009) Biomineralization ability and interaction of mineral trioxide aggregate and white Portland cement with dentin in a phosphate-containing fluid. J Endod 35:731–736CrossRefPubMed

22.

Sarkar NK, Caicedo R, Ritwik P, Moiseyeva R, Kawashima I (2005) Physiochemical basis of the biologic properties of mineral trioxide aggregate. J Endod 31:97–100CrossRefPubMed

23.

Tay FR, Pashley DH, Rueggeberg FA, Loushine RJ, Weller RN (2007) Calcium phosphate phase transformation produced by the interaction of the Portland cement component of white mineral trioxide aggregate with a phosphate-containing fluid. J Endod 33:1347–1351CrossRefPubMed

24.

Owadally, Pitt Ford TR (1994) Effect of addition of hydroxyapatite on the physical properties of IRM. Int Endod J 24:227–232CrossRef

25.

Forbes WF, Gentleman JF (1998) Risk factors, causality, and policy initiatives: the case of aluminum and mental impairment. Exp Gerontol 33:141–154CrossRefPubMed

Title: Characterization of un-hydrated and hydrated BioAggregate™ and MTA Angelus™
Authors: J. Camilleri
F. Sorrentino
D. Damidot
Publication date: 01-04-2015
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 3/2015
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-014-1292-4

At a glance: The STEP trials

Springer Medicine

Characterization of un-hydrated and hydrated BioAggregate™ and MTA Angelus™

Abstract

Objectives

Methods

Results

Conclusions

Clinical significance

At a glance: The STEP trials

Springer Medicine

Abstract

Objectives

Methods

Results

Conclusions

Clinical significance

Please log in to get access to this content

Other articles of this Issue 3/2015

Aberrant expression of p-Smad3 in oral carcinogenesis

Dental fear in children: the role of previous negative dental experiences

Color alteration, hydrogen peroxide diffusion, and cytotoxicity caused by in-office bleaching protocols

Increased prevalence of celiac disease in patients with oral lichen planus

Mechanical loading influences the effects of bisphosphonates on human periodontal ligament fibroblasts

Reliability of recordings of subgingival calculus detected using an ultrasonic device