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BMC Oral Health

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Open Access 01-12-2018 | Research article

Characterization of the apical bridge barrier formed following amelogenin apexification

Authors: Maha M. F. Mounir, Jamila M. A. Farsi, Turki Y. Alhazzazi, Moustafa A. Matar, Azza A. El-Housseiny

Published in: BMC Oral Health | Issue 1/2018

Abstract

Background

Recombinant amelogenin protein (RAP) is reported to induce complete root apex formation in dog model when used as apexification therapy. It also induces pulp regeneration in 85% of the treated group. Thus, the aim of this study was to investigate the nature of the remaining regenerated calcified tissues of the RAP group that showed no pulp regeneration compared to the calcium hydroxide treated group (CH).

Methods

A total of 240 dogs’ open apex root canals were used, after establishment of canals contamination. Canals were cleaned, irrigated, and filled with RAP as an apexification material and compared with CH. Treated teeth were assessed by H&E, trichrome staining, and/or immunohistochemistry technique, at 1, 3, and 6 months.

Results

A time-dependent increase in the calcified tissue barrier was observed in the apex of the RAP-treated group compared to the CH-treated group. The newly formed dentin in this RAP group was mainly tubular dentin and was functionally attached to the bone by periodontal ligament, while the CH group showed dentin-associated mineralized tissue (DAMT) associated with the newly formed apical barrier.

Conclusions

Out results suggest that RAP can be used as novel apexification material, resulting in a thickening and strengthening of the canal walls, and achieving apical closure.

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Title: Characterization of the apical bridge barrier formed following amelogenin apexification
Authors: Maha M. F. Mounir
Jamila M. A. Farsi
Turki Y. Alhazzazi
Moustafa A. Matar
Azza A. El-Housseiny
Publication date: 01-12-2018
Publisher: BioMed Central
Published in: BMC Oral Health / Issue 1/2018
Electronic ISSN: 1472-6831
DOI: https://doi.org/10.1186/s12903-018-0641-0

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Characterization of the apical bridge barrier formed following amelogenin apexification

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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