Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Clinical Phytoscience
Published in: Clinical Phytoscience 1/2019

Open Access 01-12-2019 | Original contribution

Characterization of in-vitro antioxidant, cytotoxic, thrombolytic and membrane stabilizing potential of different extracts of Cheilanthes tenuifolia and Stigmasterol isolation from n-hexane extract

Authors: Ananna Mahfuz, Fayad Bin Abdus Salam, Kanij Nahar Deepa, A. H. M. Nazmul Hasan

Published in: Clinical Phytoscience | Issue 1/2019

Login to get access

Abstract

Background

Cheilanthes tenuifolia, a member of the Pteridaceae family, is an evergreen and small fern could be abundant of bioactive compounds. The present study was designed to investigate its many therapeutic properties and isolation of bioactive compounds from extracts of Cheilanthes tenuifolia.

Methods

The dried coarse plant powder was extracted with methanol and dried with rotary evaporator. The extract was further partitioned according to the increasing polarity: N-hexane < chloroform < ethyl-acetate < methanol by modified Kupchan method. Then each extract fractions were investigated for their pharmacologic properties. Compounds were isolated from n-hexane fraction through column chromatography, followed by TLC and structure was determined by analysis of sample using 1H-NMR and matched with published phytochemistry report.

Results

Methanol fraction of Cheilanthes tenuifolia showed highest amount of total phenol content (11.32 ± 0.28 mg/gm GAE) followed by chloroform fraction (9.71 ± 0.31 mg/gm GAE) > N-hexane fraction (6.69 ± 0.67 mg/gm GAE) > ethyl acetate fraction (5.36 ± 0.54 mg/gm GAE). The methanol fraction of Cheilanthes tenuifolia possessed highest amount (7.11 ± 0.52 mg/gm QE) of total flavonoid content. Our present study indicates that methanol extract was most potent (IC50 = 9.926 μg/ml) inhibitor of DPPH free radicals. In brine shrimp lethality bio assay, all the extracts showed dose dependent increment of mortality and chloroform extract was found most cytotoxic (LC50 = 34.493 μg/ml) compared to other plant extracts. The chloroform fraction of Cheilanthes tenuifolia was most potent in terms of thrombolytic activity. A compound was isolated (CT-2) using column chromatography followed by TCL and PTLC (35% pet ether in CHCl3) and analyzed by 1H-NMR. The structure of stigmasterol was confirmed by comparing the 1H-NMR data with previously published phytochemistry report.

Conclusion

Cheilanthes tenuifolia could be a potential candidate for bioactive compounds and further studies on isolation and characterization of its bioactive compounds are highly required.
Literature
1.
Karthik V, Raju K, Ayyanar M, Gowrishankar K, Sekar T. Ethnomedicinal uses of Pteridophytes in Kolli Hills, eastern Ghats of Tamil Nadu, India. J Nat Prod Plant Resour. 2011;1(2):50–5.
2.
3.
Ferdousi A, Rahman MO, Hassan MA. Seed germination behaviour of six medicinal plants from Bangladesh. Bangl J Plant Taxon. 2014;21(1):71–6.CrossRef
4.
Shah R, Islam M, Rabbi F, Shova NA, Akter A, Akter H, et al. Phytotherapeutic practices of a folk medicinal practitioner in Dinajpur district, Bangladesh. J Appl Pharm Sci. 2017;7(05):161–5.
5.
Khatun M, Rahman M, Haque T, Rahman MM, Akter M, Akter S, Jhumur A. Cytotoxicity potentials of eleven Bangladeshi medicinal plants. Sci World J. 2014;2014:1–7.CrossRef
6.
Jarial R, Shard A, Thakur S, Sakinah M, Zularisam AW, Rezania S, et al. Characterization of flavonoids from fern Cheilanthes tenuifolia and evaluation of antioxidant, antimicrobial and anticancer activities. J King Saud Univ Sci. 2018;30(1):425–32.CrossRef
7.
Sen K. MukhopadhyayR. New report of vessel elements in Aleuritopteris and Cheilanthes. Taiwania. 2014;59(3):231–9.
8.
Augustin N, Thomas B. Medico-potential ferns of Angamaly region, Ernakulam district, Kerala, India. IJCPCR. 2015;5(4):207–11.
9.
Hanum F, Hamzah N. The use of medicinal plant species by the Temuan tribe of Ayer Hitam. Pertanika J Trop Agric Sci. 1999;22(2):85–94.
10.
Benniamin A. Medicinal ferns of north eastern India with special reference to Arunachal Pradesh. Indian J Tradit Know. 2011;10(3):516–22.
11.
Wagenen BCV, Larsen R, Cardellina JH, Ran D, Lidert ZC, Swithenbank C. Ulosantoin, a potent insecticide from the sponge Ulosaruetzleri. J Org Chem. 1993;58(2):335–7.CrossRef
12.
Senguttuvan J, Paulsamy S, Karthika K. Phytochemical analysis and evaluation of leaf and root parts of the medicinal herb, Hypochaeris radicata L. for in vitro antioxidant activities. Asian Pac J Trop Biomed. 2014;4(1):359–67.CrossRef
13.
Meena H, Pandey HK, Pandey P, Arya MC, Ahmed Z. Evaluation of antioxidant activity of two important memory enhancing medicinal plants Baccopa monnieri and Centella asiatica. Indian J Pharmacol. 2012;44(1):114–7.CrossRef
14.
Harbertson JF. Spayd. Measuring phenolics in the winery. Am J Enol Viticult. 2006;57(3):280–8.
15.
Jiao H, Wang SY. Correlation of antioxidant capacities to oxygen radical scavenging enzyme activities in blackberry. J Agric Food Chem. 2000;48(11):5672–6.CrossRef
16.
Bristy NJ, Hasan AN, Alam MN, Wahed TB, Roy P, Alam KK. Characterization of antioxidant and cytotoxic potential of methanolic extracts of different parts of Aegle marmelos (L.). Int J Pharm Sci Res. 2017;8(3):1476–84.
17.
Brand-Williams W, Cuvelier ME, Berst C. Use of free radical method to evaluate antioxidant activity. LebensmWiss Technol. 1995;28:25–30.
18.
Hussain F, Islam MA, Bulbul L, Moghal MR, Hossain MS. In vitro thrombolytic potential of root extracts of four medicinal plants available in Bangladesh. Anc Sci Life. 2014;33(3):162–4.PubMed
19.
Omale J, Okafor PN. Comparative antioxidant capacity, membrane stabilization, polyphenol composition and cytotoxicity of the leaf and stem of Cissus multistriata. Afr J Biotechnol. 2008;7(17):3129–33.
20.
Meyer BN, Ferringni NR, Puam JE, Lacobsen LB, Nichols DE. In vitro antibacterial, cytotoxic and free radical scavenging activities of an Aspergillus species. Drug Info J. 1982;31:516–54.
21.
Coskun O. Separation techniques: chromatography. North Clin Istanbul. 2016;3(2):156–60.
22.
Danot M, Nahmias S, Zoller U. An undergraduate column chromatography experiment. J Chem Educ. 1984;61(11):1019.CrossRef
23.
Bajpai VK, Majumder R, Park JG. Isolation and purification of plant secondary metabolite using column chromatographic technique. Bangladesh J Pharmacol. 2016;11:844–8.CrossRef
24.
Chaturvedula VP, Prakash I. Isolation of Stigmasterol and β-Sitosterol from the dichloromethane extract of Rubus suavissimus. IntCurr Pharm J. 2012;1(9):239–42.
25.
Dhar KS, Wahed TB, Hasan AN, Wahed SB. In vitro antioxidant activities and cytotoxicity study of the methanolic extract of barks of Syzygium cymosum. Int J Pharm Sci Res. 2016;7(3):1021–5.
26.
Hossain MI, Sakib MH, Mahmood AA, Karim N, Alam MS, Islam MA, Sharma M. Study on in-vitro thrombolytic activity of methanolic extract of Mesua ferrea leaves. Int J Med Health Res. 2015;1(2):52–5.
27.
Shahriar M, Khair NZ, Akhter R, Chowdhury SF. In vitro membrane stabilizing activity of Erythrina variegate bark. J Chem Pharm Res. 2015;7(4):960–2.
28.
Sarah QS, Anny FC, Misbahuddin M. Brine shrimp lethality assay. Bangladesh J Pharmacol. 2017;12(2):5–9.CrossRef
29.
Prabha PS, Chaithanya KK, Hagos Z, Nagaraju B, Gopalakrishnan VK. Isolation and identification of bioactive compound from Ipomoea obscura (L.) Ker Gawl. J Pharm Res. 2017;11(1):10–4.
Metadata
Title
Characterization of in-vitro antioxidant, cytotoxic, thrombolytic and membrane stabilizing potential of different extracts of Cheilanthes tenuifolia and Stigmasterol isolation from n-hexane extract
Authors
Ananna Mahfuz
Fayad Bin Abdus Salam
Kanij Nahar Deepa
A. H. M. Nazmul Hasan
Publication date
01-12-2019
Publisher
Springer Berlin Heidelberg
Published in
Clinical Phytoscience / Issue 1/2019
Electronic ISSN: 2199-1197
DOI
https://doi.org/10.1186/s40816-019-0135-x

Other articles of this Issue 1/2019

Clinical Phytoscience 1/2019 Go to the issue

Original contribution

Hepatoprotective studies on methanolic extract fractions of Lindernia ciliata and development of qualitative analytical profile for the bioactive extract

Original contribution

Assessing the bioefficacy of conventional solvent and supercritical fluid extracts of green tea to alleviate lifestyle related dysfunctions

Original contribution

Solanum nigrum confers protection against CCl4-induced experimental hepatotoxicity by increasing hepatic protein synthesis and regulation of energy metabolism

Original contribution

Bioefficacy of red cabbage against hypercholesterolemic diet mediated oxidative stress

Original contribution

Anti-nociceptive and anti-inflammatory effects of an aqueous extract of blended leaves of Ocimum gratissimum and Psidium guajava

Correction

Correction to: Cytogenotoxic and mutagenic profiling of cashew nut shell liquids and cardanol