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Archives of Gynecology and Obstetrics

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01-09-2016 | Gynecologic Endocrinology and Reproductive Medicine

Characterization of endometriosis-associated immune cell infiltrates (EMaICI)

Authors: Claudia Scheerer, Petia Bauer, Vito Chiantera, Jalid Sehouli, Andreas Kaufmann, Sylvia Mechsner

Published in: Archives of Gynecology and Obstetrics | Issue 3/2016

Abstract

Objective

To identify and characterize endometriosis-associated immune cell infiltrates (EMaICI). Furthermore, to define occurrence and size of EMaICI in various types of endometriosis.

Methods

Immune cells were characterized in samples of 60 premenopausal women with histological proven endometriosis. Therefore, immunohistochemical staining with monoclonal antibodies for CD3, CD4, CD8, CD45RO, CD25, CD56, CD68, and CD20 on sections of paraffin-embedded endometriotic tissue was performed.

Results

EMaICI were observed in all the types of endometriosis, and characterized as T lymphocytes (CD3+), helper T lymphocytes (CD4+), cytotoxic T lymphocytes (CD8+), antigen-experienced T lymphocytes”memory cells” (CD45RO+), macrophages (CD68+), and B lymphocytes (CD20+). The maximum frequency of EMaICI and their distribution per endometriotic lesion (EML) was observed in peritoneal endometriosis (pEM) and in ovarian endometriosis (Ov. EM). In myometrium from adenomyosis (M/AM), EMaICI occurrence was lower and smaller in size in comparison with EMaICI seen in other forms of endometriosis. EMaICI were negative for regulatory T cells (CD25+ high, FoxP3+) and natural killer cells (NK cells, CD56+).

Conclusion

Numerous and brisk EMaICI comprising several types of immune cells in all endometriosis forms suggest acute immunological reactions within the microenvironment of endometriosis lesions.

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Title: Characterization of endometriosis-associated immune cell infiltrates (EMaICI)
Authors: Claudia Scheerer
Petia Bauer
Vito Chiantera
Jalid Sehouli
Andreas Kaufmann
Sylvia Mechsner
Publication date: 01-09-2016
Publisher: Springer Berlin Heidelberg
Published in: Archives of Gynecology and Obstetrics / Issue 3/2016
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-016-4142-6

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Characterization of endometriosis-associated immune cell infiltrates (EMaICI)

Abstract

Objective

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Methods

Results

Conclusion

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