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Lasers in Medical Science
Published in: Lasers in Medical Science 3/2011

01-05-2011 | Original Article

Characterization of dental caries by LIF spectroscopy with 404-nm excitation

Authors: Shiny Sara Thomas, J. L. Jayanthi, Narayanan Subhash, Joji Thomas, Rupananda J. Mallia, G. N. Aparna

Published in: Lasers in Medical Science | Issue 3/2011

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Abstract

The potential of laser-induced fluorescence (LIF) spectroscopy for the characterization of different stages of dental caries using 404-nm diode laser excitation was investigated. In vitro spectra from 16 sound, 10 noncavitated carious and 10 cavitated carious molar teeth were recorded on a miniature fibre-optic spectrometer. The areas under the receiver operating characteristics (ROC-AUC) were calculated and one-way analysis of variance (ANOVA) was performed. The LIF spectra of the carious teeth showed two peaks at 635 and 680 nm in addition to a broad band seen at 500 nm in sound teeth. The fluorescence intensity ratios, F500/F635 and F500/F680, in carious teeth were always lower than those in sound teeth. The ROC-AUC for discriminating between carious and sound teeth was 0.94, and for discriminating between noncavitated and cavitated carious teeth was 0.87. Statistically significant differences (p<0.001) were seen between sound, noncavitated carious and cavitated carious teeth. The results showed that LIF spectroscopy has the potential to be useful for characterizing different stages of caries in a clinical setting.
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Metadata
Title
Characterization of dental caries by LIF spectroscopy with 404-nm excitation
Authors
Shiny Sara Thomas
J. L. Jayanthi
Narayanan Subhash
Joji Thomas
Rupananda J. Mallia
G. N. Aparna
Publication date
01-05-2011
Publisher
Springer-Verlag
Published in
Lasers in Medical Science / Issue 3/2011
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-010-0771-3

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