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Molecular Cancer
Published in: Molecular Cancer 1/2015

Open Access 01-12-2015 | Letter to the Editor

Characterization of 46 patient-specific BCR-ABL1 fusions and detection of SNPs upstream and downstream the breakpoints in chronic myeloid leukemia using next generation sequencing

Authors: Jana Linhartova, Lenka Hovorkova, Simona Soverini, Adela Benesova, Monika Jaruskova, Hana Klamova, Jan Zuna, Katerina Machova Polakova

Published in: Molecular Cancer | Issue 1/2015

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Abstract

In chronic myeloid leukemia, the identification of individual BCR-ABL1 fusions is required for the development of personalized medicine approach for minimal residual disease monitoring at the DNA level. Next generation sequencing (NGS) of amplicons larger than 1000 bp simplified and accelerated a process of characterization of patient-specific BCR-ABL1 genomic fusions. NGS of large regions upstream and downstream the individual breakpoints in BCR and ABL1 genes, respectively, also provided information about the sequence variants such are single nucleotide polymorphisms.
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Metadata
Title
Characterization of 46 patient-specific BCR-ABL1 fusions and detection of SNPs upstream and downstream the breakpoints in chronic myeloid leukemia using next generation sequencing
Authors
Jana Linhartova
Lenka Hovorkova
Simona Soverini
Adela Benesova
Monika Jaruskova
Hana Klamova
Jan Zuna
Katerina Machova Polakova
Publication date
01-12-2015
Publisher
BioMed Central
Published in
Molecular Cancer / Issue 1/2015
Electronic ISSN: 1476-4598
DOI
https://doi.org/10.1186/s12943-015-0363-8

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