Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Chinese Medicine
Published in: Chinese Medicine 1/2018

Open Access 01-12-2018 | Research

Characterization and anti-tumor activity of a polysaccharide isolated from Dendrobium officinale grown in the Huoshan County

Authors: Yuan Wei, Linwei Wang, Dujun Wang, Dan Wang, Chongwei Wen, Bangxin Han, Zhen Ouyang

Published in: Chinese Medicine | Issue 1/2018

Login to get access

Abstract

Background

Polysaccharides are carbohydrate chains composed of linked monosaccharide units. Accumulating studies report that polysaccharides isolated from Dendrobium officinale have a variety of functions. However, the composition and anti-tumor activity of D. officinale grown in the Huoshan area are largely unknown.

Methods

A polysaccharide (DOPA-1) was isolated from D. officinale by hot water extraction and ethanol precipitation, followed by purification via DEAE-cellulose and Sephadex G-100 chromatography. DOPA-1 was analyzed by infrared and nuclear magnetic resonance and then characterized by periodate oxidation and Smith degradation. The anti-tumor activity of DOPA-1 was then tested in HepG-2 cells.

Results

Our results show that DOPA-1 is mainly comprised of mannose, glucose, and galactose at a molar ratio of 1:0.42:0.27 and has an average molecular weight of 2.29 × 105 Da. Additionally, DOPA-1 inhibited HepG-2 cell growth in a dose-dependent manner. DOPA-1-treated HepG-2 cells also had increased reactive oxygen species (ROS) levels and decreased mitochondrial membrane potential. Furthermore, apoptosis was observed in DOPA-1-treated HepG-2 cells along with Bcl-2 downregulation and Bax upregulation at the protein level.

Conclusions

Our findings suggest that DOPA-1 induces apoptosis in tumor cells via altered mitochondrial function, ROS production, and altered apoptosis-related protein expression. This bioactive polysaccharide could, therefore, potentially be further developed as an anti-tumor adjuvant drug.
Literature
1.
Zhao P, Wang W, Feng FS, Wu F, Yang ZQ, Wang WJ. High-frequency shoot regeneration through transverse thin cell layer culture in Dendrobium Candidum Wall Ex Lindl. Plant Cell Tissue Organ Cult. 2007;90:131.CrossRef
2.
Wang K, Sui DJ, Wang CS, Yang L, Ouyang Z, Chen NF, et al. Protective effects of five different types of Dendrobium on CCl4-induced liver injury in mice. China J Chin Mater Medica. 2017;42:1945.
3.
4.
Cao YJ, Pu ZJ, Tang YP, Shen J, Chen YY, Kang A, et al. Advances in bio-active constituents, pharmacology and clinical applications of rhubarb. Chin Med. 2017;12:36.CrossRefPubMedPubMedCentral
5.
Lee CT, Kuo HC, Chen YH, Tsai MY. Current advances in the biological activity of polysaccharides in Dendrobium with intriguing therapeutic potential. Curr Med Chem. 2018;24:1.
6.
Ji YB, Dong F, Gao SY, Zou X. Apoptosis induced by Capparis spionosa polysaccharide in human HepG2. Chin Tradit Herb Drugs. 2008;39:1364–7.
7.
Shen CY, Zhang WL, Jiang JG. Immune-enhancing activity of polysaccharides from Hibiscus sabdariffa Linn. via MAPK and NF-kB signaling pathways in RAW264.7 cells. J Funct Foods. 2017;34:118–29.CrossRef
8.
Olatunji OJ, Yan F, Olatunji OO, Jian T, Yuan W, Zhen O, et al. Polysaccharides purified from Cordyceps cicadae protects PC12 cells against glutamate-induced oxidative damage. Carbohydr Polym. 2016;153:187–95.CrossRefPubMed
9.
Li F, Luo P, Liu H. A potential adjuvant agent of chemotherapy: sepia ink polysaccharides. Mar Drugs. 2018;16:485.
10.
Huang K, Li Y, Tao S, Wei G, Huang Y, Chen D, et al. Purification, characterization and biological activity of polysaccharides from Dendrobium officinale. Molecules. 2016;21:701.CrossRef
11.
He TG, Yang LT, Li YR. Physicochemical properties and antitumor activity of polysaccharide DCPP1a-1 from suspension-cultured protocorms of Dendrobium candidum. Nat Prod Res Dev. 2007;19:410–4.
12.
Luo QL, Tang ZH, Zhang XF, Zhong YH, Yao SZ, Wang LS, et al. Chemical properties and antioxidant activity of a water-soluble polysaccharide from Dendrobium officinale. Int J Biol Macromol. 2016;89:219–27.CrossRefPubMed
13.
He TB, Huang YP, Yang L, Liu TT, Gong WY, Wang XJ, et al. Structural characterization and immunomodulating activity of polysaccharide from Dendrobium officinale. Int J Biol Macromol. 2016;83:34–41.CrossRefPubMed
14.
Chen Y, Yang JK. Research progress of treatment of traditional Chinese medicine for liver cancer. J Liaoning Univ Trad Chin Med. 2010;4:951.
15.
Luo HL, Cai TY, Chen QL, Huang MQ, Mei CE, Li YQ. Enhancement of Dendrobium candidum polysaccharide on killing effect of LAK cells of umbilical cord blood and peripheral blood of cancer patients in vitro. Chin J Cancer. 2000;19:1124–6.
16.
Zha XQ. Polysaccharides in Laminaria japonica (LP): extraction, physicochemical properties and their hypolipidemic activities in diet-induced mouse model of atherosclerosis. Food Chem. 2012;134:244–52.CrossRef
17.
Bai R, Yuling MA, Zhang P, Wang YP, Li YD, Hu FD. Content determination of saccharide in polysaccharides containing galacturonic acid by phenol-sulfuric acid method combined with calibration factor method. Chin Pharm. 2017;28:2974–8.
18.
Shi J, Cheng C, Zhao H, Jing J, Gong N, Lu W. In vivo anti-radiation activities of the Ulva pertusa polysaccharides and polysaccharide-iron(III) complex. Int J Biol Macromol. 2013;60:341–6.CrossRefPubMed
19.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65:55.CrossRefPubMed
20.
Son YO, Lee JC, Hitron JA, Pan J, Zhang Z, Shi X. Cadmium induces intracellular Ca2+- and H2O2-dependent apoptosis through JNK- and p53-mediated pathways in skin epidermal cell line. Toxicol Sci Off J Soc Toxicol. 2010;113:127.CrossRef
21.
Ma L, Qin C, Wang M, Gan D, Cao L, Ye H, et al. Preparation, preliminary characterization and inhibitory effect on human colon cancer HT-29 cells of an acidic polysaccharide fraction from Stachys floridana Schuttl. ex Benth. Food Chem Toxicol. 2013;60:269–76.CrossRefPubMed
22.
Smiley ST, Reers M, Mottola-Hartshorn C, Lin M, Chen A, Smith TW, et al. Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1. Proc Natl Acad Sci USA. 1991;88:3671–5.CrossRefPubMed
23.
Nie S, Cui SW, Xie M, Phillips AO, Phillips GO. Bioactive polysaccharides from Cordyceps sinensis: isolation, structure features and bioactivities. Bioact Carbohydr Diet Fibre. 2013;1:38–52.CrossRef
24.
Wasser SP. Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl Microbiol Biotechnol. 2002;60:258–74.CrossRefPubMed
25.
Mizuno T, Hagiwara T, Nakamura T, Ito H, Shimura K, Sumiya T, et al. Antitumor activity and some properties of water-soluble polysaccharides from “Himematsutake”, the fruiting body of Agavicus blazei Murill. Agric Biol Chem. 1990;54:2889–96.
26.
de Keizer PL, Burgering BM, Dansen TB. Forkhead box o as a sensor, mediator, and regulator of redox signaling. Antioxid Redox Signal. 2011;14:1093–106.CrossRefPubMed
27.
Seo JY, Kim SK, Nguyen PH, Lee JY, Tung P, Sung SH, et al. Chemical constituents from a Gynostemma laxumand their antioxidant and neuroprotective activities. Chin Med. 2017;12:15.CrossRefPubMedPubMedCentral
28.
Jia WX, Choi YT, Lau BS, Leung WN, Ng CF, Chan CW. Gastrodia and Uncaria (tianma gouteng) water extract exerts antioxidative and antiapoptotic effects against cerebral ischemia in vitro and in vivo. Chin Med. 2016;11:27.CrossRef
29.
Rottenberg H, Hoek JB. The path from mitochondrial ROS to aging runs through the mitochondrial permeability transition pore. Aging Cell. 2017;16:943–55.CrossRefPubMedPubMedCentral
30.
31.
Fong Y, Tang CC, Hu HT, Fang HY, Chen BH, Wu CY, et al. Inhibitory effect of trans-ferulic acid on proliferation and migration of human lung cancer cells accompanied with increased endogenous reactive oxygen species and Î2-catenin instability. Chin Med. 2016;11:45.CrossRefPubMedPubMedCentral
32.
Hata AN, Engelman JA, Faber AC. The BCL-2 family: key mediators of the apoptotic response to targeted anti-cancer therapeutics. Cancer Discov. 2015;5:475–87.CrossRefPubMedPubMedCentral
33.
Xie SZ, Liu B, Zhang DD, Zha XQ, Pan LH, Luo JP. Intestinal immunomodulating activity and structural characterization of a new polysaccharide from stems of Dendrobium officinale. Food Funct. 2016;7:2789.CrossRefPubMed
34.
Yue H, Liu Y, Qu H, Ding K. Structure analysis of a novel heteroxylan from the stem of Dendrobium officinale and anti-angiogenesis activities of its sulfated derivative. Int J Biol Macromol. 2017;103:533–42.CrossRefPubMed
Metadata
Title
Characterization and anti-tumor activity of a polysaccharide isolated from Dendrobium officinale grown in the Huoshan County
Authors
Yuan Wei
Linwei Wang
Dujun Wang
Dan Wang
Chongwei Wen
Bangxin Han
Zhen Ouyang
Publication date
01-12-2018
Publisher
BioMed Central
Published in
Chinese Medicine / Issue 1/2018
Electronic ISSN: 1749-8546
DOI
https://doi.org/10.1186/s13020-018-0205-x

Other articles of this Issue 1/2018

Chinese Medicine 1/2018 Go to the issue

Research

Anti-cancer effects of Rhizoma Curcumae against doxorubicin-resistant breast cancer cells

Review

Cellular stress response mechanisms of Rhizoma coptidis: a systematic review

Review

Approaches in studying the pharmacology of Chinese Medicine formulas: bottom-up, top-down—and meeting in the middle

Review

Systems biology approaches in the study of Chinese herbal formulae

Research

Wentong decoction cures allergic bronchial asthma by regulating the apoptosis imbalance of EOS

Research

The antityrosinase and antioxidant activities of flavonoids dominated by the number and location of phenolic hydroxyl groups