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01-01-2019 | Original Article

Characterisation of a newly detected bacteriophage infecting Bordetella bronchiseptica in swine

Authors: Yibao Chen, Lan Yang, Erchao Sun, Jiaoyang Song, Bin Wu

Published in: Archives of Virology | Issue 1/2019

Abstract

A novel virulent bacteriophage, vB_BbrM_PHB04, infecting Bordetella bronchiseptica was isolated from wastewater collected at a swine farm in China. Phage vB_BbrM_PHB04 exhibited growth over a wide range of temperature and pH conditions and showed different efficiency of plating values and lytic spectra within the same strains at 25 °C and 37 °C. High-throughput sequencing revealed that vB_BbrM_PHB04 has a linear double-stranded DNA genome with 124 putative open reading frames. Overall, the genome of vB_BbrM_PHB04 showed very low similarity (the highest nucleotide identity 82%, 1% coverage) to other phage sequences in the GenBank database. Phylogenetic analysis indicated that vB_BbrM_PHB04 is a new member of the family Myoviridae. In addition, polymerase chain reaction-based detection of phage genes in phage-resistant B. bronchiseptica variants revealed no evidence of lysogenic activity of phage vB_BbrM_PHB04.

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Title: Characterisation of a newly detected bacteriophage infecting Bordetella bronchiseptica in swine
Authors: Yibao Chen
Lan Yang
Erchao Sun
Jiaoyang Song
Bin Wu
Publication date: 01-01-2019
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 1/2019
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-018-4034-0

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