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Open Access 01-06-2016 | Research Article

Changes in the sympathetic innervation of the gut in rotenone treated mice as possible early biomarker for Parkinson’s disease

Authors: Mike Arnhold, Yanina Dening, Michaël Chopin, Esteban Arévalo, Mathias Schwarz, Heinz Reichmann, Gabriele Gille, Richard H. W. Funk, Francisco Pan-Montojo

Published in: Clinical Autonomic Research | Issue 3/2016

Abstract

Introduction

Involvement of the peripheral nervous system (PNS) is relatively common in Parkinson’s disease (PD) patients. PNS alterations appear early in the course of the disease and are responsible for some of the non-motor symptoms observed in PD patients. In previous studies, we have shown that environmental toxins can trigger the disease by acting on the enteric nervous system.

Material and methods

Here, we analyzed the effect of mitochondrial Complex I inhibition on sympathetic neuritis in vivo and sympathetic neurons in vitro. Combining in vivo imaging and protein expression profiling.

Results

we found that rotenone, a widely used mitochondrial Complex I inhibitor decreases the density of sympathetic neurites innervating the gut in vivo, while in vitro, it induces the redistribution of intracellular alpha-synuclein and neurite degeneration. Interestingly, sympathetic neurons are much more resistant to rotenone exposure than mesencephalic dopaminergic neurons.

Conclusion

Altogether, these results suggest that enteric sympathetic denervation could be an initial pre-motor alteration in PD progression that could be used as an early biomarker of the disease.

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Title: Changes in the sympathetic innervation of the gut in rotenone treated mice as possible early biomarker for Parkinson’s disease
Authors: Mike Arnhold
Yanina Dening
Michaël Chopin
Esteban Arévalo
Mathias Schwarz
Heinz Reichmann
Gabriele Gille
Richard H. W. Funk
Francisco Pan-Montojo
Publication date: 01-06-2016
Publisher: Springer Berlin Heidelberg
Published in: Clinical Autonomic Research / Issue 3/2016
Print ISSN: 0959-9851
Electronic ISSN: 1619-1560
DOI: https://doi.org/10.1007/s10286-016-0358-6

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Changes in the sympathetic innervation of the gut in rotenone treated mice as possible early biomarker for Parkinson’s disease

Abstract

Introduction

Material and methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Introduction

Material and methods

Results

Conclusion

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