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01-11-2015 | Original Contributions

Changes in Glucose Metabolism in Vertical Sleeve Gastrectomy

Authors: Yunmee Lho, Carel W. le Roux, Hyeon Soo Park, Gon Sup Kim, Jeeyoun Jung, Geum-Sook Hwang, Youn Kyoung Seo, Tae Kyung Ha, Eunyoung Ha

Published in: Obesity Surgery | Issue 11/2015

Abstract

Background

We evaluated metabolic changes after vertical sleeve gastrectomy (VSG) surgery in a rat model using proteomics and metabolomic profiling in liver and serum.

Methods

Rats were randomly divided into two groups: sham (n = 10) and VSG (n = 12). Food intake, body weight, blood glucose, insulin, and thyroid hormone levels were measured. Two-dimensional electrophoresis, nuclear resonance spectroscopy, mass spectroscopy, immunofluorescence, and immunoblot analyses were used to determine and validate changes in metabolites and proteins in liver tissue and serum samples.

Results

Food intake and body weight decreased after VSG group (p < 0.05 and p < 0.05, respectively). Random blood glucose (sham, 183.3 ± 5.6 mg/dL; VSG, 138.5 ± 3.7 mg/dL) decreased while random insulin (sham, 0.45 ± 0.16 μg/L; VSG, 1.05 ± 0.18 μg/L) increased after VSG (p < 0.05 and p < 0.01, respectively). We found that expressions of gluconeogenic enzymes (phosphoenolpyruvate carboxykinase-1 and glucose-6-phosphatase) and concentrations of pyruvate and malate decreased while lactate, NADH, NADPH, glucose, and AMP/ATP ratio increased after VSG. Thyroid hormones, triiodothyronine (T3) and free thyroxine (fT4), decreased after VSG.

Conclusion

This study proves that VSG suppresses hepatic glucose production.

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Title: Changes in Glucose Metabolism in Vertical Sleeve Gastrectomy
Authors: Yunmee Lho
Carel W. le Roux
Hyeon Soo Park
Gon Sup Kim
Jeeyoun Jung
Geum-Sook Hwang
Youn Kyoung Seo
Tae Kyung Ha
Eunyoung Ha
Publication date: 01-11-2015
Publisher: Springer US
Published in: Obesity Surgery / Issue 11/2015
Print ISSN: 0960-8923
Electronic ISSN: 1708-0428
DOI: https://doi.org/10.1007/s11695-015-1636-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Changes in Glucose Metabolism in Vertical Sleeve Gastrectomy

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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