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Obesity Surgery
Published in: Obesity Surgery 1/2018

01-01-2018 | Original Contributions

Changes in Global Transcriptional Profiling of Women Following Obesity Surgery Bypass

Authors: Marcela Augusta de Souza Pinhel, Natalia Yumi Noronha, Carolina Ferreira Nicoletti, Bruno Affonso Parente de Oliveira, Cristiana Cortes-Oliveira, Vitor Caressato Pinhanelli, Wilson Salgado Junior, Ana Julia Machry, Wilson Araújo da Silva Junior, Dorotéia Rossi Silva Souza, Júlio Sérgio Marchini, Carla Barbosa Nonino

Published in: Obesity Surgery | Issue 1/2018

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Abstract

Background

Differential gene expression in peripheral blood mononuclear cells (PBMCs) after Roux-en-Y gastric bypass (RYGB) is poorly characterized. Markers of these processes may provide a deeper understanding of the mechanisms that underlie these events. The main goal of this study was to identify changes in PBMC gene expression in women with obesity before and 6 months after RYGB-induced weight loss.

Methods

The ribonucleic acid (RNA) of PBMCs from 13 obese women was analyzed before and 6 months after RYGB; the RNA of PBMCs from nine healthy women served as control. The gene expression levels were determined by microarray analysis. Significant differences in gene expression were validated by real-time quantitative polymerase chain reaction (RT-qPCR).

Results

Microarray analysis for comparison of the pre- and postoperative periods showed that 1366 genes were differentially expressed genes (DEGs). The main pathways were related to gene transcription; lipid, energy, and glycide metabolism; inflammatory and immunological response; cell differentiation; oxidative stress regulation; response to endogenous and exogenous stimuli; substrate oxidation; mTOR signaling pathway; interferon signaling; mitogen-activated protein kinases (MAPK), cAMP response element binding protein (CREB1), heat shock factor 1 (HSF1), and sterol regulatory element binding protein 1c (SREBP-1c) gene expression; adipocyte differentiation; and methylation.

Conclusions

Six months after bariatric surgery and significant weight loss, many molecular pathways involved in obesity and metabolic diseases change. These findings are an important tool to identify potential targets for therapeutic intervention and clinical practice of nutritional genomics in obesity.
Literature
1.
Ng M, Fleming T, Robinson M, et al. Global, regional, and national prevalence of overweight and obesity in children and adults during 1980-2013: a systematic analysis for the Global Burden of Disease Study 2013. Lancet. 2014;384(9945):766–81.CrossRefPubMedPubMedCentral
2.
3.
Kelly T, Yang W, Chen CS, et al. Global burden of obesity in 2005 and projections to 2030. Int J Obes. 2008;32(9):1431–7.CrossRef
4.
De Lorenzo A, Soldati L, Sarlo F, et al. New obesity classification criteria as a tool for bariatric surgery indication. World J Gastroenterol. 2016;22(2):681–703.CrossRefPubMedPubMedCentral
5.
Kral JG, Kava RA, Catalano PM, et al. Severe obesity: the neglected epidemic. Obes Facts. 2012;5(2):254–69.CrossRefPubMed
6.
Sjöström L, Narbro K, Sjöström CD, et al. Effects of bariatric surgery on mortality in Swedish obese subjects. N Engl J Med. 2007;357(8):741–52.CrossRefPubMed
7.
Mitka M. Bariatric surgery continues to show benefits for patients with diabetes. JAMA. 2012;307(18):1901–2.CrossRefPubMed
8.
González-Plaza JJ, Gutiérrez-Repiso C, García-Serrano S, et al. Effect of Roux-en-Y gastric bypass-induced weight loss on the transcriptomic profiling of subcutaneous adipose tissue. Surg Obes Relat Dis. 2016;12(2):257–63.CrossRefPubMed
9.
Afman LA, Muller M. Human nutrigenomics of gene regulation by dietary fatty acids. Prog Lipid Res. 2012;51(1):63–70.CrossRefPubMed
10.
Fuchs D, Piller R, Linseisen J, et al. The human peripheral blood mononuclear cell proteome responds to a dietary flaxseed-intervention and proteins identified suggest a protective effect in atherosclerosis. Proteomics. 2007;7(18):3278–88.CrossRefPubMed
11.
Whitney AR, Diehn M, Popper SJ, et al. Individuality and variation in gene expression patterns in human blood. Proc Natl Acad Sci U S A. 2003;100(4):1896–901.CrossRefPubMedPubMedCentral
12.
Radich JP, Mao M, Stepaniants S, et al. Individual-specific variation of gene expression in peripheral blood leukocytes. Genomics. 2004;83(6):980–8.CrossRefPubMed
13.
de Mello VD, Kolehmanien M, Schwab U, et al. Gene expression of peripheral blood mononuclear cells as a tool in dietary intervention studies: what do we know so far? Mol Nutr Food Res. 2012;56(7):1160–72.CrossRefPubMed
14.
Moody DE. Genomics techniques: an overview of methods for the study of gene expression. J Anim Sci. 2001;79:E128–35.CrossRef
15.
Berisha SZ, Serre D, Schauer P, et al. Changes in whole blood gene expression in obese subjects with type 2 diabetes following bariatric surgery: a pilot study. PLoS One. 2011;6(3):e16729.CrossRefPubMedPubMedCentral
16.
Pena SD, Di Pietro G, Fuchshuber-Moraes M, et al. The genomic ancestry of individuals from different geographical regions of Brazil is more uniform than expected. PLoS One. 2011;6(2):e17063.CrossRefPubMedPubMedCentral
17.
Mahawar KK, Kumar P, Parmar C, et al. Small bowel limb lengths and Roux-en-Y gastric bypass: a systematic review. Obes Surg. 2016;26(3):660–71.CrossRefPubMed
18.
Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987;162(1):156–9.CrossRefPubMed
19.
Hochberg Y, Benjamini Y. More powerful procedures for multiple significance testing. Stat Med. 1990;9(7):811–8.CrossRefPubMed
20.
Searle S, Speed T, Milliken GA. Population marginal means in the linear model: an alternative to least squares means. Am Stat. 1980;34:216–21.
21.
Kupershmidt I, Su QJ, Grewal A, et al. Ontology-based meta-analysis of global collections of high-throughput public data. PLoS One. 2010;29:5(9).
22.
23.
Hindle AK, Edwards C, McCaffrey T, et al. Reactivation of adiponectin expression in obese patients after bariatric surgery. Surg Endosc. 2010;24(6):1367–73.CrossRefPubMed
24.
Hindle AK, Koury J, McCaffrey T, et al. Dysregulation of gene expression within the peroxisome proliferator activated receptor pathway in morbidly obese patients. Surg Endosc. 2009;23(6):1292–7.CrossRefPubMed
25.
Mardinoglu A, Heiker JT, Gärtner D, et al. Extensive weight loss reveals distinct gene expression changes in human subcutaneous and visceral adipose tissue. Sci Rep. 2015;5:14841.CrossRefPubMedPubMedCentral
26.
Clément K, Viguerie N, Poitou C, et al. Weight loss regulates inflammation-related genes in white adipose tissue of obese subjects. FASEB J. 2004;18(14):1657–69.CrossRefPubMed
27.
Dankel SN, Fadnes DJ, Stavrum AK, et al. Switch from stress response to homeobox transcription factors in adipose tissue after profound fat loss. PLoS One. 2010;5(6):e11033.CrossRefPubMedPubMedCentral
28.
Gerhard GS, Styer AM, Strodel WE, et al. Gene expression profiling in subcutaneous, visceral and epigastric adipose tissues of patients with extreme obesity. Int J Obes. 2014;38(3):371–8.CrossRef
29.
Han J, Li E, Chen L, et al. The CREB coactivator CRTC2 controls hepatic lipid metabolism by regulating SREBP1. Nature. 2015;524(7564):243–6.CrossRefPubMed
30.
Ma X, Xu L, Alberobello AT, et al. Celastrol protects against obesity and metabolic dysfunction through activation of a HSF1-PGC1α transcriptional axis. Cell Metab. 2015;22(4):695–708.CrossRefPubMed
31.
Gijón MA, Spencer DM, Siddiqi AR, et al. Cytosolic phospholipase A2 is required for macrophage arachidonic acid release by agonists that do and do not mobilize calcium. Novel role of mitogen-activated protein kinase pathways in cytosolic phospholipase A2 regulation. J Biol Chem. 2000;275(26):20146–56.CrossRefPubMed
32.
Gubern A, Barceló-Torns M, Barneda D, et al. JNK and ceramide kinase govern the biogenesis of lipid droplets through activation of group IVA phospholipase A2. J Biol Chem. 2009;284(47):32359–69.CrossRefPubMedPubMedCentral
33.
Khatchadourian A, Bourque SD, Richard VR, et al. Dynamics and regulation of lipid droplet formation in lipopolysaccharide (LPS)-stimulated microglia. Biochim Biophys Acta. 2012;1821(4):607–17.CrossRefPubMed
34.
35.
Sokolowska M, Chen LY, Eberlein M, et al. Low molecular weight hyaluronan activates cytosolic phospholipase A2α and eicosanoid production in monocytes and macrophages. J Biol Chem. 2014;289(7):4470–88.CrossRefPubMed
36.
Cancello R, Zulian A, Gentilini D, et al. Permanence of molecular features of obesity in subcutaneous adipose tissue of ex-obese subjects. Int J Obes. 2013;37(6):867–73.CrossRef
37.
Nicoletti CF, de Oliveira BA, de Pinhel MA, et al. Influence of excess weight loss and weight regain on biochemical indicators during a 4-year follow-up after Roux-en-Y gastric bypass. Obes Surg. 2015;25(2):279–84.CrossRefPubMed
Metadata
Title
Changes in Global Transcriptional Profiling of Women Following Obesity Surgery Bypass
Authors
Marcela Augusta de Souza Pinhel
Natalia Yumi Noronha
Carolina Ferreira Nicoletti
Bruno Affonso Parente de Oliveira
Cristiana Cortes-Oliveira
Vitor Caressato Pinhanelli
Wilson Salgado Junior
Ana Julia Machry
Wilson Araújo da Silva Junior
Dorotéia Rossi Silva Souza
Júlio Sérgio Marchini
Carla Barbosa Nonino
Publication date
01-01-2018
Publisher
Springer US
Published in
Obesity Surgery / Issue 1/2018
Print ISSN: 0960-8923
Electronic ISSN: 1708-0428
DOI
https://doi.org/10.1007/s11695-017-2828-x

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