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Cancer Cell International
Published in: Cancer Cell International 1/2021

Open Access 01-12-2021 | Cervical Cancer | Primary research

CircNEIL3 promotes cervical cancer cell proliferation by adsorbing miR-137 and upregulating KLF12

Authors: Yuan Chen, Yiting Geng, Junchao Huang, Dan Xi, Guoping Xu, Wendong Gu, Yingjie Shao

Published in: Cancer Cell International | Issue 1/2021

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Abstract

Background

CircRNAs play crucial roles in multiple tumours. However, the functions of most circRNAs in cervical cancer remain unclear.

Methods

This study collected GSE113696 data from the GEO database to search for differentially expressed circRNAs in cervical cancer. Quantitative reverse transcription PCR was used to detect the expression level of circNEIL3 in cervical cancer cells and tissues. Then, functional experiments in vitro and in vivo were performed to evaluate the effects of circNEIL3 in cervical cancer.

Results

CircNEIL3 was highly expressed in cervical cancer. In vivo and in vitro experiments verified that circNEIL3 enhanced the proliferation capacity of cervical cancer cells. RNA immunoprecipitation, luciferase reporter assay, pull-down assay, and fluorescent in situ hybridization confirmed the interaction between circNEIL3 and miR-137 in cervical cancer. A luciferase reporter assay showed that circNEIL3 adsorbed miR-137 and upregulated KLF12 to regulate the proliferation of cervical cancer cells.

Conclusions

CircNEIL3 is an oncogene in cervical cancer and might serve as a ceRNA that competitively binds to miR-137, thereby indirectly upregulating the expression of KLF12 and promoting the proliferation of cervical cancer cells.
Appendix
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Metadata
Title
CircNEIL3 promotes cervical cancer cell proliferation by adsorbing miR-137 and upregulating KLF12
Authors
Yuan Chen
Yiting Geng
Junchao Huang
Dan Xi
Guoping Xu
Wendong Gu
Yingjie Shao
Publication date
01-12-2021
Publisher
BioMed Central
Published in
Cancer Cell International / Issue 1/2021
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/s12935-020-01736-4

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