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Open Access 01-12-2023 | Cervical Cancer | Research

C/EBPβ expression decreases in cervical cancer and leads to tumorigenesis

Authors: Haichen Long, Yangyang Li, Huijuan Wang, Bingxuan Guo, Shuyan Song, Xiangyi Zhe, Hongtao Li, Dongmei Li, Renfu Shao, Zemin Pan

Published in: BMC Cancer | Issue 1/2023

Abstract

Background

Cervical cancer is currently estimated to be the fourth most common cancer among women worldwide and the leading cause of cancer-related deaths in some of the world’s poorest countries. C/EBPβ has tumor suppressor effects because it is necessary for oncogene-induced senescence. However, C/EBPβ also has an oncogenic role. The specific role of C/EBPβ in cervical cancer as a tumor suppressor or oncoprotein is unclear.

Objective

To explore the role of the C/EBPβ protein in cervical tumorigenesis and progression.

Methods

Quantitative RT-PCR was used to analyze C/EBPβ (15 cervical cancer tissue samples and 15 corresponding normal cervical tissue samples), miR-661, and MTA1 mRNA expression in clinical samples (10 cervical cancer tissue samples and 10 corresponding normal cervical tissue samples). Immunohistochemistry was used to analyze C/EBPβ (381 clinical samples), Ki67 (80 clinical samples) and PCNA ( 60 clinical samples) protein expression. MALDI-TOF MassARRAY was used to analyze C/EBPβ gene methylation (13 cervical cancer tissues and 13 corresponding normal cervical tissues). Cell proliferation was analyzed by CCK-8 in cervical cancer cell lines. Western blotting and immunohistochemistry were performed to detect C/EBPβ protein expression levels, and mRNA expression was analyzed by quantitative RT-PCR analysis. Flow cytometry was performed to measure cell cycle distribution and cell apoptosis. Colony formation, Transwell, cell invasion, and wound healing assays were performed to detect cell migration and invasion.

Results

C/EBPβ protein expression was significantly reduced in cervical cancer tissues compared with cervicitis tissues (P < 0.01). Ki67 protein and PCNA protein expression levels were significantly higher in cervical cancer tissues compared with cervicitis tissues. The rate of C/EBPβ gene promoter methylation of CpG12, 13, 14 and CpG19 in cervical cancer tissues was significantly increased compared with normal cervical tissue (P < 0.05). In addition, C/EBPβ was overexpressed in cervical cancer cells and this overexpression inhibited cell proliferation, migration, invasion, arrested cells in S phase, and promoted apoptosis.

Conclusions

We have demonstrated that C/EBPβ decreased in cervical cancer tissues and overexpression of the C/EBPβ gene in cervical cancer cells could inhibit proliferation, invasion and migration.

Available only for authorised users

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Title: C/EBPβ expression decreases in cervical cancer and leads to tumorigenesis
Authors: Haichen Long
Yangyang Li
Huijuan Wang
Bingxuan Guo
Shuyan Song
Xiangyi Zhe
Hongtao Li
Dongmei Li
Renfu Shao
Zemin Pan
Publication date: 01-12-2023
Publisher: BioMed Central
Keywords: Cervical Cancer
Cervical Cancer
Published in: BMC Cancer / Issue 1/2023
Electronic ISSN: 1471-2407
DOI: https://doi.org/10.1186/s12885-023-10543-9

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Abstract

Background

Objective

Methods

Results

Conclusions

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