Top

Archives of Gynecology and Obstetrics

Published in:

01-06-2011 | General Gynecology

Cellular proliferation in female pelvic organ prolapse: a pilot study

Authors: Peter Takacs, Cristina Saiz, Sindy Jaramillo, Yanping Zhang, Sujata Yavagal, Keith Candiotti, Carlos A. Medina

Published in: Archives of Gynecology and Obstetrics | Issue 6/2011

Abstract

Purpose

To assess cell proliferation in pelvic organ prolapse (POP).

Methods

Tissue samples of the anterior vaginal wall and uterosacral ligaments (USLs) were obtained from eight women with combined anterior vaginal wall and uterine prolapse and from eight women without POP in a standardized fashion. Immunohistochemistry against Ki-67 was used to assess cell proliferation in vaginal and USL biopsies.

Results

There were no significant differences in age, parity, menopausal status or hormone replacement therapy between the two groups. The POP-Q stage of uterine and anterior vaginal wall prolapse was significantly higher in the group of women with prolapse compared to the group without prolapse [median (range) 3 (3–4) vs. 0 (0), <0.01]. There was no significant difference between Ki-67 expressions in women with or without prolapse.

Conclusion

There were no significant differences in cell proliferation between samples from women with or without POP.

Olsen AL, Smith VJ, Bergstrom JO, Colling JC, Clark AL (1997) Epidemiology of surgically managed pelvic organ prolapse and urinary incontinence. Obstet Gynecol 89:501–506PubMedCrossRef

Kerkhof MH, Hendriks L, Brolmann HA (2009) Changes in connective tissue in patients with pelvic organ prolapse—a review of the current literature. Int Urogynecol J Pelvic Floor Dysfunct 20:461–474PubMedCrossRef

Kokcu A, Yanik F, Cetinkaya M, Alper T, Kandemir B, Malatyalioglu E (2002) Histopathological evaluation of the connective tissue of the vaginal fascia and the uterine ligaments in women with and without pelvic relaxation. Arch Gynecol Obstet 266:75–78PubMedCrossRef

Takacs P, Nassiri M, Candiotti K, Yang J, Yavagal S, Medina CA (2009) Differential expression of fibulins in the uterosacral ligaments of women with uterine prolapse. Arch Gynecol Obstet [Epub ahead of print]

Knuuti E, Kauppila S, Kotila V, Risteli J, Nissi R (2010) Genitourinary prolapse and joint hypermobility are associated with altered type I and III collagen metabolism. Arch Gynecol Obstet [Epub ahead of print]

Boreham MK, Wai CY, Miller RT, Schaffer JI, Word RA (2002) Morphometric analysis of smooth muscle in the anterior vaginal wall of women with pelvic organ prolapse. Am J Obstet Gynecol 187:56–63PubMedCrossRef

Boreham MK, Wai CY, Miller RT, Schaffer JI, Word RA (2002) Morphometric properties of the posterior vaginal wall in women with pelvic organ prolapse. Am J Obstet Gynecol 187:1501–1508 (discussion 1508–1509)PubMedCrossRef

Gabriel B, Denschlag D, Gobel H et al (2005) Uterosacral ligament in postmenopausal women with or without pelvic organ prolapse. Int Urogynecol J Pelvic Floor Dysfunct 16:475–479PubMedCrossRef

Takacs P, Nassiri M, Gualtieri M, Candiotti K, Medina CA (2009) Uterosacral ligament smooth muscle cell apoptosis is increased in women with uterine prolapse. Reprod Sci 16:447–452PubMedCrossRef

10.

Takacs P, Gualtieri M, Nassiri M, Candiotti K, Medina CA (2008) Vaginal smooth muscle cell apoptosis is increased in women with pelvic organ prolapse. Int Urogynecol J Pelvic Floor Dysfunct 19:1559–1564PubMedCrossRef

11.

Cattoretti G et al (1992) Monoclonal antibodies against recombinant parts of the Ki-67 antigen (MIB 1 and MIB 3) detect proliferating cells in microwave-processed formalin-fixed paraffin sections. J Pathol 168:357–363PubMedCrossRef

12.

Bump RC et al (1996) The standardization of terminology of female pelvic organ prolapse and pelvic floor dysfunction. Am J Obstet Gynecol 175:10–17PubMedCrossRef

13.

Bai SW, Jung YW, Kwon HS et al (2004) The role of estrogen receptor, progesterone receptor and p53 in development of stress urinary incontinence. Yonsei Med J 45(5):885–890

14.

Vincek V, Nassiri M, Nadji M, Morales AR (2003) A tissue fixative that protects macromolecules (DNA, RNA, and protein) and histomorphology in clinical samples. Lab Invest 83:1427–1435PubMedCrossRef

15.

Blakeman PJ, Hilton P, Bulmer JN (2001) Cellular proliferation in the female lower urinary tract with reference to oestrogen status. BJOG 108:813–816PubMedCrossRef

16.

Takacs P, Gualtieri M, Nassiri M, Candiotti K, Fornoni A, Medina CA (2009) Caldesmon expression is decreased in women with anterior vaginal wall prolapse: a pilot study. Int Urogynecol J Pelvic Floor Dysfunct 20:985–990PubMedCrossRef

17.

Silva-Filho AL et al (2004) Expression of p53, Ki-67, and CD31 in the vaginal margins of radical hysterectomy in patients with stage IB carcinoma of the cervix. Gynecol Oncol 95:646–654PubMedCrossRef

18.

Takacs P, Zhang Y, Jaramillo S, Bardawil T, Candiotti K, Medina C (2010) The effects of estrogen, progesterone and propylene mesh on vaginal smooth muscle cell proliferation. J Smooth Muscle Res 46(1):9–15PubMedCrossRef

19.

Ewies AA, Elshafie M, Li J et al (2008) Changes in transcription profile and cytoskeleton morphology in pelvic ligament fibroblasts in response to stretch: the effects of estradiol and levormeloxifene. Mol Hum Reprod 14:127–135PubMedCrossRef

20.

Liu YM, Choy KW, Lui WT, Pang MW, Wong YF, Yip SK (2006) 17Beta-estradiol suppresses proliferation of fibroblasts derived from cardinal ligaments in patients with or without pelvic organ prolapse. Hum Reprod 21:303–308PubMedCrossRef

21.

Connell KA, Guess MK, Chen HW, Lynch T, Bercik R, Taylor HS (2009) HOXA11 promotes fibroblast proliferation and regulates p53 in uterosacral ligaments. Reprod Sci 16:694–700PubMedCrossRef

Title: Cellular proliferation in female pelvic organ prolapse: a pilot study
Authors: Peter Takacs
Cristina Saiz
Sindy Jaramillo
Yanping Zhang
Sujata Yavagal
Keith Candiotti
Carlos A. Medina
Publication date: 01-06-2011
Publisher: Springer-Verlag
Published in: Archives of Gynecology and Obstetrics / Issue 6/2011
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-010-1565-3

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Cellular proliferation in female pelvic organ prolapse: a pilot study

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

Please log in to get access to this content

Other articles of this Issue 6/2011

RETRACTED ARTICLE: Chronical cervical infections and dysplasia (CIN I, CIN II)—vaginal vitamin D (high dose) treatment: a new effective method

Electroacupuncture for cervical ripening prior to labor induction: a randomized clinical trial

Headache in late pregnancy: a symptom for Vogt–Koyanagi–Harada disease

The prevalence of Chlamydia trachomatis and Neisseria gonorrhoeae in the endocervical swab specimens of symptomatic, asymptomatic and infertile women in Turkey

Anti-Mullerian hormone is a better marker than inhibin B, follicle stimulating hormone, estradiol or antral follicle count in predicting the outcome of in vitro fertilization

Second primary cancers following borderline ovarian tumors