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Graefe's Archive for Clinical and Experimental Ophthalmology

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01-08-2014 | Inflammatory Disorders

CD44 and RHAMM hyaluronan receptors in human ocular surface inflammation

Authors: Laura García-Posadas, Laura Contreras-Ruiz, Isabel Arranz-Valsero, Antonio López-García, Margarita Calonge, Yolanda Diebold

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 8/2014

Abstract

Background

CD44 and RHAMM hyaluronan (HA) receptors have been studied in several systemic diseases such as osteoarthritis and cancer. However, not too much is known about their role in ocular surface disorders. The purpose of this research was to determine if CD44 and RHAMM are implicated in human ocular surface inflammation.

Methods

Upper tarsal conjunctival epithelial samples from patients with active ocular surface inflammation (n = 17) and healthy controls (n = 14) were recovered by brush cytology. Patients were evaluated by an ophthalmologist and classified in different groups according to the etiology (immune atopic diseases or immune non-atopic diseases) and inflammation intensity (mild/moderate or severe). CD44, RHAMM, and p53 mRNAs were measured using real-time PCR.

Results

CD44, RHAMM, and p53 mRNAs were detected in all samples. In immune atopic diseases, higher levels of CD44 and RHAMM mRNAs were present, reaching a 300 % increase for RHAMM in severe inflammation (p < 0.001). In contrast, in immune non-atopic diseases, the HA receptors were downregulated. CD44 tended to decrease up to 30 % in severe patients (p = 0.06), and RHAMM decreased 40 % in severe inflammation (p = 0.021).

Conclusions

RHAMM may be implicated in severe ocular surface inflammation affecting the upper tarsal conjunctiva.

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Title: CD44 and RHAMM hyaluronan receptors in human ocular surface inflammation
Authors: Laura García-Posadas
Laura Contreras-Ruiz
Isabel Arranz-Valsero
Antonio López-García
Margarita Calonge
Yolanda Diebold
Publication date: 01-08-2014
Publisher: Springer Berlin Heidelberg
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 8/2014
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-014-2686-3

At a glance: The STEP trials

Springer Medicine

CD44 and RHAMM hyaluronan receptors in human ocular surface inflammation

Abstract

Background

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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