Published in:
01-07-2017 | Original Article
CD4+ memory T cells retain surface expression of CD31 independently of thymic function in patients with lymphoproliferative disorders following autologous hematopoietic stem-cell transplantation
Authors:
Egor V. Batorov, Marina A. Tikhonova, Irina V. Kryuchkova, Vera V. Sergeevicheva, Svetlana A. Sizikova, Galina Y. Ushakova, Dariya S. Batorova, Andrey V. Gilevich, Alexander A. Ostanin, Ekaterina Y. Shevela, Elena R. Chernykh
Published in:
International Journal of Hematology
|
Issue 1/2017
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Abstract
High-dose chemotherapy with autologous hematopoietic stem-cell transplantation (AHSCT) causes severe and long-lasting immunodeficiency in patients with lymphoproliferative disorders. The thymus begins to restore the T-cell repertoire approximately from the sixth month post-transplant. We assessed the dynamics of post-transplant recovery of CD4+CD45RA+CD31+ T cells, “recent thymic emigrants” (RTEs), and a poorly described subtype of CD4+CD45RA−CD31+ T cells in 90 patients with lymphoproliferative disorders following high-dose chemotherapy with AHSCT. Relative and absolute counts of CD4+CD31+ naïve and memory T cells were evaluated before AHSCT, at the day of engraftment, and 6- and 12-month post-transplant. The pre-transplant count of CD4+CD45RA+CD31+ T cells was lower than in healthy controls, and did not reach donors’ values during the 12-month period. The pre-transplant number of CD4+CD45RA–CD31+ T cells was higher than in healthy controls and was restored rapidly following AHSCT. Post-transplant mediastinal radiotherapy reduced counts of RTEs and elongated recovery period. Non-thymic tissue irradiation did not reduce this subset. The obtained data indicate that homeostatic proliferation may decrease the significance of CD31 expression on CD4+CD45RA+ T cells as a marker of RTEs, and suggest that evaluation of RTEs recovery by flow cytometry requires an accurate gating strategy to exclude CD31+ memory T cells.