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Graefe's Archive for Clinical and Experimental Ophthalmology

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01-10-2007 | Laboratory Investigation

CD4⁺CD25⁺Tregs express an increased LAG-3 and CTLA-4 in anterior chamber-associated immune deviation

Authors: Xuefei Zhu, Peizeng Yang, Hongyan Zhou, Bing Li, Xiangkun Huang, Qianli Meng, Li Wang, Aize Kijlstra

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 10/2007

Abstract

Background

Regulatory CD4⁺CD25⁺ T cells have been proven to be essential for maintenance of peripheral tolerance and autoimmune diseases. ACAID is a model of immune privilege in the eye. Relatively little is known about the role and phenotype of these regulatory CD4⁺CD25⁺ T cells in ACAID.

Methods

Injection of OVA into the anterior chamber of BALB/C mice was performed to induce ACAID. The frequencies of splenic CD4⁺CD25⁺ Tregs and the expression of CTLA-4 and LAG-3 on these cells were determined by flow cytometry. Magnetic cell sorting was used to isolate CD4⁺CD25⁺ and CD4⁺CD25⁻T cells. The function of CD4⁺CD25⁺ T cells was detected by in vitro immunosuppression assays and in vivo adoptive transfer.

Results

ACAID was successfully induced following an i.c. injection of OVA. Frequencies of CD4⁺CD25⁺ and Tregs were significantly increased in ACAID mice as compared to those in controls. The CD4⁺CD25⁺ T cells stimulated with OVA in ACAID mice showed a stronger suppressive ability in vitro than those seen in non-ACAID mice. CD4⁺CD25⁺ T cells from ACAID mice, but not from non-ACAID mice, were able to suppress DTH responses in an antigen-specific manner following adoptive transfer. The frequencies of CTLA-4 or LAG-3 on Tregs in ACAID mice were higher as compared with those in naive mice.

Conclusion

Splenic CD4⁺CD25⁺Foxp3⁺T cells expressing CTLA4 and LAG3 play an important role in the induction of ACAID.

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Title: CD4+CD25+Tregs express an increased LAG-3 and CTLA-4 in anterior chamber-associated immune deviation
Authors: Xuefei Zhu
Peizeng Yang
Hongyan Zhou
Bing Li
Xiangkun Huang
Qianli Meng
Li Wang
Aize Kijlstra
Publication date: 01-10-2007
Publisher: Springer-Verlag
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 10/2007
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-007-0591-8

Keynote webinar | Spotlight on medication adherence

Springer Medicine

CD4⁺CD25⁺Tregs express an increased LAG-3 and CTLA-4 in anterior chamber-associated immune deviation

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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