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01-10-2013 | Original Article

CD15s is a potential biomarker of serious bacterial infection in infants admitted to hospital

Authors: Josko Markic, Ana Jeroncic, Denis Polancec, Nada Bosnjak, Anita Markotic, Julije Mestrovic, Vedrana Cikes Culic

Published in: European Journal of Pediatrics | Issue 10/2013

Abstract

Early recognition of serious bacterial infection (SBI) in children is essential for better treatment outcome. Flow cytometry analysis of neutrophil surface molecules has been more frequently utilized as a tool for diagnosis of infection. The infants (n = 105) under 6 months of age presenting to the pediatric emergency department with fever without apparent source who were hospitalized with suspicion of having SBI were enrolled in this prospective study. Sixty-nine infants were included into the training pool and were classified into bacterial or viral infection group. Validation pool consisted of 36 infants. The values of white blood cells counts, absolute neutrophil count (ANC), C-reactive protein (CRP), procalcitonin (PCT), neutrophil CD11b, CD15s and CD64 expression, and the percentage (%CD15s+) and absolute count (AC-CD15s+) of CD15s+ neutrophils were determined. In infants with SBI, %CD15s+ was 10.5 times more likely to be higher than the cut-off value. ANC, CRP, PCT, CD64, and AC-CD15s+ were also found as useful biomarkers for differentiation between bacterial and viral infection. The best fit multivariate logistic regression model included CRP, PCT, and %CD15s+ as strong predictors of SBI. The model's sensitivity (87 %) and specificity (83 %) indicated high model's accuracy. After validation on independent dataset, model's accuracy maintained high: 86 % sensitivity and 93 % specificity, confirming its reliability and supporting CRP, PCT, and %CD15s+ as real predictors. The findings of this study support assumption made in the literature on significance of CD15s in inflammation processes. Also, this study demonstrated for the first time that CD15s is potentially valuable biomarker of SBI in infants.

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Title: CD15s is a potential biomarker of serious bacterial infection in infants admitted to hospital
Authors: Josko Markic
Ana Jeroncic
Denis Polancec
Nada Bosnjak
Anita Markotic
Julije Mestrovic
Vedrana Cikes Culic
Publication date: 01-10-2013
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Pediatrics / Issue 10/2013
Print ISSN: 0340-6199
Electronic ISSN: 1432-1076
DOI: https://doi.org/10.1007/s00431-013-2047-y

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CD15s is a potential biomarker of serious bacterial infection in infants admitted to hospital

Abstract

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Abstract

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