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24-02-2023 | Original Article

Carcinogenic risk in the biliary epithelium of children with congenital biliary dilatation via the DNA damage repair pathway

Authors: Hiroki Mori, Takayuki Noma, Yuji Morine, Hiroki Ishibashi, Mitsuo Shimada

Published in: Surgery Today | Issue 10/2023

Abstract

Purposes

Congenital biliary dilatation (CBD) is a high-risk factor for biliary tract cancer (BTC). We previously reported the potential for carcinogenesis in the biliary epithelium of patients with CBD. In this study, we investigated potential carcinogenetic pathways, focusing on the DNA damage repair response, in children with CBD and compared the findings with those in adults.

Methods

We enrolled 6 children with CBD and 10 adults with CBD without BTC who underwent extrahepatic bile duct resections, plus 4 control patients who underwent pancreaticoduodenectomy for non-biliary cancer. Levels of phosphorylated histone H2AX (γH2AX), MRE11, and Ku-70 in the biliary tract epithelium were evaluated by immunohistochemistry.

Results

The levels of γH2AX, MRE11, and Ku-70 were significantly higher in the gallbladder epithelium and bile duct epithelium of both children and adults than in controls.

Conclusions

Children and adults with CBD might develop BTC via the DNA damage repair pathway, as evidenced by increased γH2AX, MRE11, and Ku-70 expression.

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Title: Carcinogenic risk in the biliary epithelium of children with congenital biliary dilatation via the DNA damage repair pathway
Authors: Hiroki Mori
Takayuki Noma
Yuji Morine
Hiroki Ishibashi
Mitsuo Shimada
Publication date: 24-02-2023
Publisher: Springer Nature Singapore
Published in: Surgery Today / Issue 10/2023
Print ISSN: 0941-1291
Electronic ISSN: 1436-2813
DOI: https://doi.org/10.1007/s00595-023-02664-2

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Carcinogenic risk in the biliary epithelium of children with congenital biliary dilatation via the DNA damage repair pathway

Abstract

Purposes

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purposes

Methods

Results

Conclusions

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