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Cancer Cell International
Published in: Cancer Cell International 1/2020

Open Access 01-12-2020 | Cancer Immunotherapy | Review

CRISPR-Cas, a robust gene-editing technology in the era of modern cancer immunotherapy

Authors: Seyed Mohammad Miri, Elham Tafsiri, William Chi Shing Cho, Amir Ghaemi

Published in: Cancer Cell International | Issue 1/2020

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Abstract

Cancer immunotherapy has been emerged as a promising strategy for treatment of a broad spectrum of malignancies ranging from hematological to solid tumors. One of the principal approaches of cancer immunotherapy is transfer of natural or engineered tumor-specific T-cells into patients, a so called “adoptive cell transfer”, or ACT, process. Construction of allogeneic T-cells is dependent on the employment of a gene-editing tool to modify donor-extracted T-cells and prepare them to specifically act against tumor cells with enhanced function and durability and least side-effects. In this context, CRISPR technology can be used to produce universal T-cells, equipped with recombinant T cell receptor (TCR) or chimeric antigen receptor (CAR), through multiplex genome engineering using Cas nucleases. The robust potential of CRISPR-Cas in preparing the building blocks of ACT immunotherapy has broaden the application of such therapies and some of them have gotten FDA approvals. Here, we have collected the last investigations in the field of immuno-oncology conducted in partnership with CRISPR technology. In addition, studies that have addressed the challenges in the path of CRISPR-mediated cancer immunotherapy, as well as pre-treatment applications of CRISPR-Cas have been mentioned in detail.
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Metadata
Title
CRISPR-Cas, a robust gene-editing technology in the era of modern cancer immunotherapy
Authors
Seyed Mohammad Miri
Elham Tafsiri
William Chi Shing Cho
Amir Ghaemi
Publication date
01-12-2020
Publisher
BioMed Central
Published in
Cancer Cell International / Issue 1/2020
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/s12935-020-01546-8

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