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European Journal of Medical Research

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Open Access 01-12-2015 | Research

Calorie restriction inhibits ovarian follicle development and follicle loss through activating SIRT1 signaling in mice

Authors: Wei-Juan Liu, Xing-Mei Zhang, Na Wang, Xiao-Ling Zhou, Yu-Cai Fu, Li-Li Luo

Published in: European Journal of Medical Research | Issue 1/2015

Abstract

Background

Silent information regulator 2 related enzyme 1 (SIRT1) is one of the key factors in the mechanism of calorie restriction (CR) extending lifespan of animals. The aim of the study is to investigate if CR prolongs ovarian lifespan in mice through activating SIRT1 signaling.

Methods

In the present study, 21 female C57BL/6 mice were divided into three groups: the control (n = 7), CR (n = 7), and SRT1720 (n = 7) groups. After the 26-week treatment, the number of ovarian follicles at each stage was counted, and Western blot was performed.

Results

The number of surviving follicles in ovaries of the SRT1720 group was less than that of the CR group but more than that of the normal control (NC) group. The number of atretic follicles in the ovaries of the SRT1720 group was similar to that of the CR group but less than that of the NC group. The number of primordial follicles in the ovaries of the SRT1720 group was less than that of the CR group but more than that of the NC group. The numbers of primary follicles, secondary follicles, antral follicles, and corpora lutea in the SRT1720 group were similar to those in the CR group. Western blot analysis showed that the expression of SIRT1, SIRT6, FOXO3a, and NRF1 proteins was upregulated, and p53 was downregulated in both the CR group and the SRT1720 group compared to the control group.

Conclusions

Our results indicate that CR inhibits the activation of primordial follicles and development of follicles at different stages, thus preserving the reserve of follicle pool (at least partly) through activating SIRT1 signaling.

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Title: Calorie restriction inhibits ovarian follicle development and follicle loss through activating SIRT1 signaling in mice
Authors: Wei-Juan Liu
Xing-Mei Zhang
Na Wang
Xiao-Ling Zhou
Yu-Cai Fu
Li-Li Luo
Publication date: 01-12-2015
Publisher: BioMed Central
Published in: European Journal of Medical Research / Issue 1/2015
Electronic ISSN: 2047-783X
DOI: https://doi.org/10.1186/s40001-015-0114-8

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Calorie restriction inhibits ovarian follicle development and follicle loss through activating SIRT1 signaling in mice

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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