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BMC Oral Health
Published in: BMC Oral Health 1/2023

Open Access 01-12-2023 | CAD and CAM | Research

Biocompatibility and biofilm formation on conventional and CAD/CAM provisional implant restorations

Authors: Tipparat Parakaw, Nisarat Ruangsawasdi, Pornpen Dararat, Chareerut Phruksaniyom, Sirada Srihirun, Pobploy Petchmedyai

Published in: BMC Oral Health | Issue 1/2023

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Abstract

Dental implant treatment is a complex and sophisticated process, and implant provisional restorations play a vital role in ensuring its success. The advent of computer-aided design and computer-aided manufacturing (CAD/CAM) technology has revolutionized the field of implant restorations by providing improved precision leading to a reduction in chair time and more predictable treatment outcomes. This technology offers a promising solution to the drawbacks of conventional methods and has the potential to transform the way implant procedures are approached. Despite the clear advantages of CAD/CAM over conventional provisional implant restorations including higher accuracy of fit and superior mechanical properties, little research has been conducted on the biological aspect of these novel restorations. This study aims to fill that gap, comprehensively assessing the biocompatibility, gingival tissue attachment and biofilm formation of a range of provisional implant restorations using CAD/CAM technology through milling and 3-D printing processes compared to conventional fabrication. The biocompatibility of the tested restorations was assessed by MTT assay, Calcein-AM assay as well as SEM analysis. The surface roughness of the tested samples was evaluated, alongside the attachment of Human Gingival Fibroblasts (HGF) cells as well as biofilm formation, and estimated Porphyromonas gingivalis (P. gingivalis) cell count from DNA detection.
The results showed all tested provisional implant restorations were non-toxic and good HGF cell attachment but differed in their quantity of biofilm formation, with surface texture influenced by the material and fabrication technique, playing a role. Within the limitation of this study, the findings suggest that CAD/CAM-fabricated provisional implant restorations using a milling technique may be the most favourable among tested groups in terms of biocompatibility and periodontal-related biofilm formation.
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Metadata
Title
Biocompatibility and biofilm formation on conventional and CAD/CAM provisional implant restorations
Authors
Tipparat Parakaw
Nisarat Ruangsawasdi
Pornpen Dararat
Chareerut Phruksaniyom
Sirada Srihirun
Pobploy Petchmedyai
Publication date
01-12-2023
Publisher
BioMed Central
Keyword
CAD and CAM
Published in
BMC Oral Health / Issue 1/2023
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/s12903-023-03468-z

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