Top

Published in:

Open Access 01-12-2014 | Research article

Btbd7 contributes to reduced E-cadherin expression and predicts poor prognosis in non-small cell lung cancer

Authors: Chuifeng Fan, Yuan Miao, Xiupeng Zhang, Di Liu, Guiyang Jiang, Xuyong Lin, Qiang Han, Lan Luan, Zhonghai Xu, Enhua Wang

Published in: BMC Cancer | Issue 1/2014

Abstract

Background

Disorders of cell adhesion are critical steps in cancer progression in which varieties of markers including cadherins are involved in.Btbd7 was found to inhibit E-cadherin expression in MDCK cells and play important roles during branching morphogenesis of embryonic salivary glands and lungs. However its function in malignant tumors is largely unknown. The aim of this study is to investigate the clinicopathological significance and possible function of Btbd7 in non-small cell lung cancer.

Methods

Immunohistochemistry and Western blotting were used to investigate Btbd7 expression in non-small cell lung cancer and lung tissues. The clinicopathological association and the overall survival was analyzed. In vitro experiments were performed using siRNA to investigate the function of Btbd7 in lung cancer cells.

Results

Btbd7 expression was elevated in non-small cell lung cancer tissues compared to normal lung tissues. Increased Btbd7 expression was significantly associated with lymph node metastasis, reduced E-cadherin expression and patients’ poor clinical outcome. Downregulation of Btbd7 expression in lung cancer cells by siRNA significantly inhibits cancer cell invasion and effectively restores E-cadherin expression in cancer cell membrane.

Conclusions

Btbd7 contributes to reduced expression of E-cadherin and may be a promising cancer marker in non-small cell lung cancer.

Available only for authorised users

Zhang PX, Wang Y, Liu Y, Jiang GY, Li QC, Wang EH: P120-catenin isoform 3 regulates subcellular localization of Kaiso and promotes invasion in lung cancer cells via a phosphorylation-dependent mechanism. Int J Oncol. 2011, 38 (6): 1625-1635.PubMed

Yu JH, Miao Y, Xu HT, Liu Y, Jiang GY, Stoecker M, Wang ED, Wang EH: N-Terminal 1–54 amino acid sequence and armadillo repeat domain rre indispensable for p120-catenin isoform 1A in regulating E-cadherin. PloS One. 2012, 7 (5): e37008-10.1371/journal.pone.0037008.CrossRefPubMedPubMedCentral

Mays RW, Nelson WJ, Marrs JA: Generation of epithelial cell polarity: roles for protein trafficking, membrane-cytoskeleton, and E-cadherin-mediated cell adhesion. Cold Spring Harb Symp Quant Biol. 1995, 60: 763-773. 10.1101/SQB.1995.060.01.082.CrossRefPubMed

Mărgineanu E, Cotrutz CE, Cotrutz C: Correlation between E-cadherin abnormal expressions in different types of cancer and the process of metastasis. Rev Med Chir Soc Med Nat Iasi. 2008, 112 (2): 432-436.PubMed

Buda A, Pignatelli M: E-cadherin and the cytoskeletal network in colorectal cancer development and metastasis. Cell Commun Adhes. 2011, 18 (6): 133-143. 10.3109/15419061.2011.636465.CrossRefPubMed

Kondo K, Kohno N, Yokoyama A, Hiwada K: Decreased MUC1 expression induces E-cadherin-mediated cell adhesion of breast cancer cell lines. Cancer Res. 1998, 58 (9): 2014-2019.PubMed

El-Hariry I, Pignatelli M, Lemoine NR: FGF-1 and FGF-2 modulate the E-cadherin/catenin system in pancreatic adenocarcinoma cell lines. Br J Cancer. 2001, 84 (12): 1656-1663. 10.1054/bjoc.2001.1813.CrossRefPubMed

Jiao W, Miyazaki K, Kitajima Y: Inverse correlation between E-cadherin and Snail expression in hepatocellular carcinoma cell lines in vitro and in vivo. Br J Cancer. 2002, 86 (1): 98-101. 10.1038/sj.bjc.6600017.CrossRefPubMedPubMedCentral

Pujuguet P, Del Maestro L, Gautreau A, Louvard D, Arpin M: Ezrin regulates E-cadherin-dependent adherens junction assembly through Rac1 activation. Mol Biol Cell. 2003, 14 (5): 2181-2191. 10.1091/mbc.E02-07-0410.CrossRefPubMedPubMedCentral

10.

Yang JY, Zong CS, Xia W, Wei Y, Ali-Seyed M, Li Z, Broglio K, Berry DA, Hung MC: MDM2 promotes cell motility and invasiveness by regulating E-cadherin degradation. Mol Cell Biol. 2006, 26 (19): 7269-7282. 10.1128/MCB.00172-06.CrossRefPubMedPubMedCentral

11.

Dupre-Crochet S, Figueroa A, Hogan C, Ferber EC, Bialucha CU, Adams J, Richardson EC, Fujita Y: Casein kinase 1 is a novel negative regulator of E-cadherin-based cell-cell contacts. Mol Cell Biol. 2007, 27 (10): 3804-3816. 10.1128/MCB.01590-06.CrossRefPubMedPubMedCentral

12.

Lioni M, Brafford P, Andl C, Rustgi A, El-Deiry W, Herlyn M, Smalley KS: Dysregulation of claudin-7 leads to loss of E-cadherin expression and the increased invasion of esophageal squamous cell carcinoma cells. Am J Pathol. 2007, 170 (2): 709-721. 10.2353/ajpath.2007.060343.CrossRefPubMedPubMedCentral

13.

Chetty R, Jain D, Serra S: P120 catenin reduction and cytoplasmic relocalization leads to dysregulation of E-cadherin in solid pseudopapillary tumors of the pancreas. Am J Clin Pathol. 2008, 130 (1): 71-76. 10.1309/FEYD99TXC4LMYVA5.CrossRefPubMed

14.

Hage B, Meinel K, Baum I, Giehl K, Menke A: Rac1 activation inhibits E-cadherin-mediated adherens junctions via binding to IQGAP1 in pancreatic carcinoma cells. Cell Commun Signal. 2009, 7: 23-10.1186/1478-811X-7-23.CrossRefPubMedPubMedCentral

15.

Onodera T, Sakai T, Hsu JC, Matsumoto K, Chiorini JA, Yamada KM: Btbd7 regulates epithelial cell dynamics and branching morphogenesis. Science. 2010, 329 (5991): 562-565. 10.1126/science.1191880.CrossRefPubMedPubMedCentral

16.

Roberto PT, Daisuke Y, Pierre D: Born to bind:the BTB protein–protein interaction domain. BioEssays. 2006, 28: 1194-1202. 10.1002/bies.20500.CrossRef

17.

Goldstraw P, Crowley J, Chansky K: The IASLC lung cancer staging project: proposals forthe revision of the TNM stage groupings in the forthcoming (7th) edition of the TNM classification of malignant tumours. J Thorac Oncol. 2007, 2: 706-714. 10.1097/JTO.0b013e31812f3c1a.CrossRefPubMed

18.

Travis WD, Muller-Hermelink HK, Harris CC: World Health Organization Classification of Tumors: Pathology and Genetics of Tumors of the Lung, Pleura, Thymus and Heart. 2004, Lyon: IARC

19.

Travis WD, Brambilla E, Noguchi M: International association for the study of lung cancer/American thoracic society/European respiratory society international multidisciplinary classification of lung adenocarcinoma. J Thorac Oncol. 2011, 6: 244-285. 10.1097/JTO.0b013e318206a221.CrossRefPubMedPubMedCentral

20.

Jawhari A, Farthing M, Pignatelli M: The importance of the E-cadherin-catenin complex in the maintenance of intestinal epithelial homoeostasis: more than intercellular glue?. Gut. 1997, 41 (5): 581-584. 10.1136/gut.41.5.581.CrossRefPubMedPubMedCentral

21.

Pallier K, Cessot A, Côté JF, Just PA, Cazes A, Fabre E, Dane C, Riquet M, Devouassoux-Shisheboran M, Ansieau S, Puisieux A, Laurent-Puig P, Blons H: TWIST1 a new determinant of epithelial to mesenchymal transition in EGFR mutated lung adenocarcinoma. PLoS One. 2012, 7 (1): e29954-10.1371/journal.pone.0029954.CrossRefPubMedPubMedCentral

The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/14/704/prepub

Title: Btbd7 contributes to reduced E-cadherin expression and predicts poor prognosis in non-small cell lung cancer
Authors: Chuifeng Fan
Yuan Miao
Xiupeng Zhang
Di Liu
Guiyang Jiang
Xuyong Lin
Qiang Han
Lan Luan
Zhonghai Xu
Enhua Wang
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: BMC Cancer / Issue 1/2014
Electronic ISSN: 1471-2407
DOI: https://doi.org/10.1186/1471-2407-14-704

Webinar | 19-02-2024 | 17:30 (CET)

Keynote webinar | Spotlight on antibody–drug conjugates in cancer

Watch now

Antibody–drug conjugates (ADCs) are novel agents that have shown promise across multiple tumor types. Explore the current landscape of ADCs in breast and lung cancer with our experts, and gain insights into the mechanism of action, key clinical trials data, existing challenges, and future directions.

Dr. Véronique Diéras

Prof. Fabrice Barlesi

Developed by: Springer Medicine

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

Please log in to get access to this content

Other articles of this Issue 1/2014

The biography of the immune system and the control of cancer: from St Peregrine to contemporary vaccination strategies

The effects of psychological interventions on depression and anxiety among Chinese adults with cancer: a meta-analysis of randomized controlled studies

Identification of FISH biomarkers to detect chromosome abnormalities associated with prostate adenocarcinoma in tumour and field effect environment

Polysialic acid is associated with better prognosis and IDH1-mutation in diffusely infiltrating astrocytomas

Prolonged complete response after treatment withdrawal in HER2-overexpressed, hormone receptor-negative breast cancer with liver metastases: the prospect of disappearance of an incurable disease

Combination of expression levels of miR-21 and miR-126 is associated with cancer-specific survival in clear-cell renal cell carcinoma

Keynote webinar | Spotlight on antibody–drug conjugates in cancer