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Intensive Care Medicine
Published in: Intensive Care Medicine 12/2009

01-12-2009 | Pediatric Original

Bronchoalveolar lavage fluid peptidomics suggests a possible matrix metalloproteinase-3 role in bronchopulmonary dysplasia

Authors: Giovanni Vento, Chiara Tirone, Paola Lulli, Ettore Capoluongo, Franco Ameglio, Simona Lozzi, Francesco Cota, Fabio Mosca, Costantino Romagnoli, Irene Messana, Massimo Castagnola, Rosanna Inzitari

Published in: Intensive Care Medicine | Issue 12/2009

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Abstract

Background

Bronchoalveolar lavage fluid (BALF) is an important diagnostic source to investigate molecular changes occurring in lung disorders. The objective of this study was to assess and compare the peptidomic profiles of BALF from premature neonates with and without bronchopulmonary dysplasia (BPD).

Methods

Samples were obtained on the 3rd day of life from 34 neonates with gestational age ≤32 weeks. Two pools of samples from patients with and without BPD were analyzed by high performance liquid chromatography. Several differentially expressed peptides were collected and sequenced. Moreover, samples from single donors were analyzed by liquid chromatography-electrospray ionization mass spectrometry to define the molecular mass values of various peptides and to quantify their expression. Levels of some matrix metalloproteinases and their tissue inhibitors were also determined in single samples.

Results

Neonates of the BPD group (N = 16) showed significantly lower mean gestational age and birth weight with respect to the no-BPD group (N = 18; P < 0.0001). Levels of six peptides were significantly higher in BPD patients (P < 0.05). Two of them were identified as the albumin fragments 1–21 (2,428 Da) and 399–406 (956 Da). Levels of matrix metalloproteinase-3 (MMP-3) enzyme probably involved in albumin fragment generation were also significantly higher in the BPD group compared to the no-BPD group (P < 0.05), whereas the levels of tissue inhibitor of metalloproteinases-1 were significantly lower (P < 0.05). Levels of albumin fragments and MMP-3 showed a significant correlation (P < 0.05).

Conclusions

This study shows that proteomic techniques can be applied to investigate the involvement of proteolytic enzymes on the airways of mechanically ventilated premature infants.
Appendix
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Metadata
Title
Bronchoalveolar lavage fluid peptidomics suggests a possible matrix metalloproteinase-3 role in bronchopulmonary dysplasia
Authors
Giovanni Vento
Chiara Tirone
Paola Lulli
Ettore Capoluongo
Franco Ameglio
Simona Lozzi
Francesco Cota
Fabio Mosca
Costantino Romagnoli
Irene Messana
Massimo Castagnola
Rosanna Inzitari
Publication date
01-12-2009
Publisher
Springer-Verlag
Published in
Intensive Care Medicine / Issue 12/2009
Print ISSN: 0342-4642
Electronic ISSN: 1432-1238
DOI
https://doi.org/10.1007/s00134-009-1646-6

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