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Cancer Cell International
Published in: Cancer Cell International 1/2020

01-12-2020 | Breast Cancer | Primary research

Roles of eIF3m in the tumorigenesis of triple negative breast cancer

Authors: Wei Han, Cong Zhang, Chun-tao Shi, Xiao-jiao Gao, Ming-hui Zhou, Qi-xiang Shao, Xiao-jun Shen, Cheng-jiang Wu, Fang Cao, Yong-wei Hu, Jian-liang Yuan, Hou-zhong Ding, Qing-hua Wang, Hao-nan Wang

Published in: Cancer Cell International | Issue 1/2020

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Abstract

Background

Without targets, triple negative breast cancer (TNBC) has the worst prognosis in all subtypes of breast cancer (BC). Recently, eukaryotic translation initiation factor 3 m (eIF3m) has been declared to be involved in the malignant progression of various neoplasms. The aim of this study is to explore biological functions of eIF3m in TNBC.

Methods

Multiple databases, including Oncomine, KM-plotter and so on, were performed to analyze prognosis and function of eIF3m in TNBC. After transfection of eIF3m-shRNA lentivirus, CCK-8, colony formation assay, cell cycle analysis, wound healing assay, transwell assays, mitochondrial membrane potential assay and cell apoptosis analysis were performed to explore the roles of eIF3m in TNBC cell bio-behaviors. In addition, western blotting was conducted to analyze the potential molecular mechanisms of eIF3m.

Results

In multiple databases, up-regulated eIF3m had lower overall survival, relapse-free survival and post progression survival in BC. EIF3m expression in TNBC was obviously higher than in non-TNBC or normal breast tissues. Its expression in TNBC was positively related to differentiation, lymph node invasion and distant metastasis. After knockdown of eIF3m, cell proliferation, migration, invasion and levels of mitochondrial membrane potential of MDA-MB-231 and MDA-MB-436 were all significantly suppressed, while apoptosis rates of them were obviously increased. In addition, eIF3m could regulate cell-cycle, epithelial–mesenchymal transition and apoptosis-related proteins. Combined with public databases and RT-qPCR, 14 genes were identified to be modulated by eIF3m in the development of TNBC.

Conclusions

eIF3m is an unfavorable indicator of TNBC, and plays a vital role in the process of TNBC tumorigenesis.
Appendix
Available only for authorised users
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Metadata
Title
Roles of eIF3m in the tumorigenesis of triple negative breast cancer
Authors
Wei Han
Cong Zhang
Chun-tao Shi
Xiao-jiao Gao
Ming-hui Zhou
Qi-xiang Shao
Xiao-jun Shen
Cheng-jiang Wu
Fang Cao
Yong-wei Hu
Jian-liang Yuan
Hou-zhong Ding
Qing-hua Wang
Hao-nan Wang
Publication date
01-12-2020
Publisher
BioMed Central
Published in
Cancer Cell International / Issue 1/2020
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/s12935-020-01220-z

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