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BMC Cancer
Published in: BMC Cancer 1/2020

01-12-2020 | Breast Cancer | Research article

Methylation of WT1, CA10 in peripheral blood leukocyte is associated with breast cancer risk: a case-control study

Authors: Anqi Ge, Song Gao, Yupeng Liu, Hui Zhang, Xuan Wang, Lei Zhang, Da Pang, Yashuang Zhao

Published in: BMC Cancer | Issue 1/2020

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Abstract

Background

Studies have shown that abnormal changes of specific-gene DNA methylation in leukocytes may be associated with an elevated risk of cancer. However, associations between the methylation of the zinc-related genes, WT1 and CA10, and breast cancer risk remain unknown.

Methods

The methylation of WT1 and CA10 was analyzed by methylation-sensitive high-resolution-melting (MS-HRM) in a case-control study with female subjects (N = 959). Logistic regression was used to analyze the associations, and propensity score (PS) method was used to adjust confounders.

Results

The results showed that WT1 hypermethylation was associated with an increased risk of breast cancer, with an odds ratio (OR) of 3.07 [95% confidence interval (CI): 1.67–5.64, P < 0.01]. Subgroup analyses showed that WT1 hypermethylation was specifically associated with an elevated risk of luminal A subtype (OR = 2.62, 95% CI: 1.11–6.20, P = 0.03) and luminal B subtype (OR = 3.23, 95% CI: 1.34–7.80, P = 0.01). CA10 hypermethylation was associated with an increased risk of luminal B subtype (OR = 1.80, 95% CI: 1.09–2.98, P = 0.02).

Conclusion

The results of the present study suggest that the hypermethylation of WT1 methylation in leukocytes is significantly associated with an increased risk of breast cancer. The hypermethylation of WT1 is associated with an increased risk of luminal subtypes of breast cancer, and the hypermethylation of CA10 is associated with an increased risk of luminal B subtype of breast cancer.
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Metadata
Title
Methylation of WT1, CA10 in peripheral blood leukocyte is associated with breast cancer risk: a case-control study
Authors
Anqi Ge
Song Gao
Yupeng Liu
Hui Zhang
Xuan Wang
Lei Zhang
Da Pang
Yashuang Zhao
Publication date
01-12-2020
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2020
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/s12885-020-07183-8

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