Published in:
01-03-2000 | Meeting abstract
Breast cancer in young women: prevalence of LOH at p53, BRCA1 and BRCA2
Authors:
SM Johnson, JA Shaw, RA Walker
Published in:
Breast Cancer Research
|
Special Issue 1/2000
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Excerpt
Previous studies have shown that breast cancers show more aggressive pathological features in younger women than those occurring in older women. These findings have raised the question whether differences are present at the molecular level. In order to examine genetic alterations associated with early onset breast cancer 31 cases, selected for age under 35 at diagnosis, were examined for loss of heterozygosity (LOH) and microsatellite instability (MI) in three key chromosomal intervals: 17p13 (p53), 17q11-22 (BRCA1) and 13q12-14 (BRCA2). The cases selected had no obvious family history. DNA was extracted from formalin-fixed paraffin embedded normal and tumour tissue (whole section and microdissected DNA) and analysed by PCR amplification of microsatellite repeat markers. Products were resolved on 10% non-denaturing polyacrylamide gels and silver stained. …
