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24-08-2023 | Botulinum Toxin | Original Articles

Histomorphometric Changes of the Masseter Muscle of Rats After a Single Injection of Botulinum Toxin Type A

Authors: Douglas Massoni Ramos, Raira de Brito Silva, Giancarlo De la Torre Canales, Luciana Resende, Marcelo Augusto Marretto Esquisatto, Nádia Cristina Fávaro Moreira, Malin Ernberg, Célia Marisa Rizzatti-Barbosa

Published in: Aesthetic Plastic Surgery | Issue 5/2024

Abstract

Background

It has been reported that botulinum toxin type A (BoNT-A) produces structural changes in masticatory muscles. However, not all histomorphometric parameters affected by BoNT-A parameters have been assessed. This study investigated the histomorphometric changes in the masseter muscle of rats after a single injection of BoNT-A.

Methods

Forty-four adult animals were randomly divided into control group (n = 22) and BoNT-A group (n = 22). Controls received a single dose of 0.14 mL/kg of saline in masseter muscles, and the BoNT-A group received a 7 U/Kg of BoNT-A. The groups received the same volume of injected substances. Animals were sacrificed on 7th (n = 5), 14th (n = 5), 21st (n = 5), 28th (n = 4) and 90th (n = 3) days post-treatment. Histological masseter tissue slides were obtained from hematoxylin–eosin treatment and analyzed in optical microscopy regarding muscle cross-sectional area, amount of connective tissue and quantity and diameter of myocytes. For statistical analysis, generalized linear models were used to compare the data (ANOVA). In all test, the significance level of 5% was set.

Results

BoNT-A values of cross-sectional area of the masseter muscle were significantly lower than controls (p < 0.01) throughout the study. Regarding myocytes quantity, BoNT-A subgroups presented higher values than controls (p < 0.0001) since the 14th day until the end of the study; however, the diameter of myocytes was smaller in all BoNT-A subgroups (p < 0.0001) in all assessment points. The amount of connective tissue was higher in BoNT-A subgroups (p < 0.0001) throughout the study.

Conclusion

A single injection of BoNT-A altered the structure of masseter muscle of rats, regarding its histomorphometric parameters.

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Title: Histomorphometric Changes of the Masseter Muscle of Rats After a Single Injection of Botulinum Toxin Type A
Authors: Douglas Massoni Ramos
Raira de Brito Silva
Giancarlo De la Torre Canales
Luciana Resende
Marcelo Augusto Marretto Esquisatto
Nádia Cristina Fávaro Moreira
Malin Ernberg
Célia Marisa Rizzatti-Barbosa
Publication date: 24-08-2023
Publisher: Springer US
Keyword: Botulinum Toxin
Published in: Aesthetic Plastic Surgery / Issue 5/2024
Print ISSN: 0364-216X
Electronic ISSN: 1432-5241
DOI: https://doi.org/10.1007/s00266-023-03572-z

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Histomorphometric Changes of the Masseter Muscle of Rats After a Single Injection of Botulinum Toxin Type A

Abstract

Background

Methods

Results

Conclusion

No Level Assigned

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

No Level Assigned

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