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Open Access 01-11-2017 | Original Article

Bone resorptive activity in symptomatic and asymptomatic apical lesions of endodontic origin

Authors: M. Salinas-Muñoz, M. Garrido-Flores, M. Baeza, P. Huamán-Chipana, J. García-Sesnich, R. Bologna, R. Vernal, M. Hernández

Published in: Clinical Oral Investigations | Issue 8/2017

Abstract

Objectives

The aim of this study is to assess the levels and diagnostic accuracy of a set of bone resorption biomarkers, including TRAP-5, RANKL, and OPG in symptomatic and asymptomatic apical lesions and controls.

Materials and methods

Apical tissues from symptomatic and asymptomatic apical periodontitis patients and periodontal ligaments from healthy teeth extracted for orthodontic reasons were processed for tissue homogenization and the levels of TRAP-5, RANKL, and OPG were determined by multiplex assay. Marker levels were analyzed by Kruskal Wallis test, and diagnostic accuracy was analyzed with ROC curves.

Results

Higher levels of RANKL, OPG, and RANKL/OPG ratio were determined in both types of apical lesions compared to healthy periodontal ligament, whereas higher TRAP-5 levels were found only in symptomatic apical lesions (p < 0.05). OPG, RANKL, and RANKL/OPG ratio showed diagnostic potential to identify apical lesions versus healthy controls (AUC = 0.69, p < 0.05); while TRAP-5 showed a potential to discriminate symptomatic versus asymptomatic apical periodontitis (AUC = 0.71, p < 0.05) and healthy controls (AUC = 0.83, p < 0.05).

Conclusions

Apical lesions showed higher RANKL and OPG levels than healthy tissues. TRAP-5 levels were the highest in symptomatic apical lesions, suggesting that these represent a progressive state, and showed diagnostic potential.

Clinical relevance

Clinically symptomatic apical periodontitis might represent biologically progressive apical lesions based on TRAP5 levels. TRAP5 has diagnostic potential to identify these lesions, representing a candidate prognostic biomarker.

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Title: Bone resorptive activity in symptomatic and asymptomatic apical lesions of endodontic origin
Authors: M. Salinas-Muñoz
M. Garrido-Flores
M. Baeza
P. Huamán-Chipana
J. García-Sesnich
R. Bologna
R. Vernal
M. Hernández
Publication date: 01-11-2017
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 8/2017
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-017-2062-x

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Bone resorptive activity in symptomatic and asymptomatic apical lesions of endodontic origin

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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