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Archives of Gynecology and Obstetrics

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01-01-2017 | Gynecologic Endocrinology and Reproductive Medicine

Biological and biomechanical analysis of two types of mesenchymal stem cells for intervention in chemotherapy-induced ovarian dysfunction

Authors: Yuan Pan, Liqun Zhang, Xinyue Zhang, Cong Hu, Ruizhi Liu

Published in: Archives of Gynecology and Obstetrics | Issue 1/2017

Abstract

Purpose

The study aim was to investigate the biological and biomechanical features of a chemotherapy-induced ovarian dysfunction (CIOD) rat model after intervention with human umbilical cord mesenchymal stem cells (UC-MSCs) and human amniotic mesenchymal stem cells (h-AMSCs), thus providing a biological and biomechanical research basis for clinical treatment.

Methods

The serum levels of estradiol (E2), follicle-stimulating hormone (FSH), luteinizing hormone (LH), and vascular endothelial growth factor (VEGF) in the rat CIOD models were evaluated on the 14th, 30th, 60th, and 90th day of intervention with h-AMSCs and UC-MSCs. In addition, the ovaries in each group were sampled on the 14th and 90th day of intervention for tissue morphology and tensile testing.

Results

The serum levels of E2, LH, and VEGF in the h-AMSC and UC-MSC groups were greater than in the model group, but the serum FSH level was less than in the model group, and the differences were significant (P < 0.05); the maximum tensile stress and maximum strain in h-AMSC and UC-MSC groups were significantly greater than in the model group (P < 0.05).

Conclusions

UC-MSC and h-AMSC intervention restored damaged ovarian morphology, elasticity, and toughness to a certain extent, and ovarian function showed some recovery.

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Title: Biological and biomechanical analysis of two types of mesenchymal stem cells for intervention in chemotherapy-induced ovarian dysfunction
Authors: Yuan Pan
Liqun Zhang
Xinyue Zhang
Cong Hu
Ruizhi Liu
Publication date: 01-01-2017
Publisher: Springer Berlin Heidelberg
Published in: Archives of Gynecology and Obstetrics / Issue 1/2017
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-016-4224-5

At a glance: The STEP trials

Springer Medicine

Biological and biomechanical analysis of two types of mesenchymal stem cells for intervention in chemotherapy-induced ovarian dysfunction

Abstract

Purpose

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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