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01-07-2006 | Original Paper

Biological activity of paediatric cerebral cavernomas: an immunohistochemical study of 28 patients

Authors: Wuttipong Tirakotai, Sandra Fremann, Niels Soerensen, Wolfgang Roggendorf, Adrian M. Siegel, Hans Dieter Mennel, Yuan Zhu, Helmut Bertalanffy, Ulrich Sure

Published in: Child's Nervous System | Issue 7/2006

Abstract

Objective

According to the hypothesis that paediatric cerebral cavernomas may have different biological activity compared to adult cavernomas, immunohistochemical analysis was used to elucidate the biological nature of paediatric cavernomas.

Patients and methods

We examined the histological features and the proliferative and angiogenic capacity of the tissue specimens acquired from 28 paediatric patients. Normal paediatric brain tissues obtained from paediatric autopsy cases were used as a control group. The proliferative activity of the endothelium and the neoangiogenetic capacity were investigated by immunohistochemistry for proliferating cell nuclear antigen (PCNA), Ki-67 epitope (MIB-1), Flk-1 receptor, vascular endothelial growth factor (VEGF), hypoxia-inducible factor (HIF)-1 α, and endoglin antibody, respectively. Afterwards, the results of the paediatric lesions were analysed and compared with the correspondent values of previously reported immunohistochemical analysis in adult cavernomas.

Results

Positive immunostaining of VEGF was detected significantly less in paediatric cavernomas compared to adult cases (p<0.05). In contrast, endoglin, a protein that is upregulated during an increased vascular shear stress, was expressed more often in paediatric cavernomas (p<0.05). Neither the expression of the PCNA nor the expression of the HIF-1α was found significantly different between paediatric and adult cavernomas. However, the positive immunoreaction for MIB-1 occurred more often in the paediatric cases (p<0.05).

Conclusions

The immunohistochemical study indicates that paediatric cavernomas are dynamic lesions. The VEGF/Flk-1 associated neoangiogenesis may play a minor role for the biology of paediatric cavernomas, while endoglin seems to act more prominently than previously thought, particularly for the biology of paediatric cavernomas.

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Title: Biological activity of paediatric cerebral cavernomas: an immunohistochemical study of 28 patients
Authors: Wuttipong Tirakotai
Sandra Fremann
Niels Soerensen
Wolfgang Roggendorf
Adrian M. Siegel
Hans Dieter Mennel
Yuan Zhu
Helmut Bertalanffy
Ulrich Sure
Publication date: 01-07-2006
Publisher: Springer-Verlag
Published in: Child's Nervous System / Issue 7/2006
Print ISSN: 0256-7040
Electronic ISSN: 1433-0350
DOI: https://doi.org/10.1007/s00381-006-0044-4

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Springer Medicine

Biological activity of paediatric cerebral cavernomas: an immunohistochemical study of 28 patients

Abstract

Objective

Patients and methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Patients and methods

Results

Conclusions

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