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Journal of Assisted Reproduction and Genetics

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Open Access 01-02-2017 | Review

Biobanking of different body fluids within the frame of IVF—a standard operating procedure to improve reproductive biology research

Authors: Michael Schenk, Berthold Huppertz, Barbara Obermayer-Pietsch, Darja Kastelic, Martina Hörmann-Kröpfl, Gregor Weiss

Published in: Journal of Assisted Reproduction and Genetics | Issue 2/2017

Abstract

Purpose

The aim of the present study was to develop a standard operating procedure (SOP) for the collection, transport, and storage of human cumulus cells, follicular fluid, blood serum, seminal plasma, embryo culture supernatant, and embryo culture supernatant control obtained within the IVF process under approved protocols and written informed consent from participating patients. The SOP was developed at the Kinderwunsch Institut Schenk, Dobl, Austria, together with Biobank Graz of the Medical University of Graz, Austria.

Methods

The SOP provides comprehensive details of laboratory procedures and sampling of the different fluids within the IVF process. Furthermore, information on sample coding, references of involved laboratory techniques (e.g., oocyte retrieval with a Steiner-TAN needle), ethical approvals, and biobanking procedures are presented.

Results

The result of the present study is a standard operating procedure.

Conclusions

The SOP ensures a professional way for collection and scientific use of IVF samples by the Kinderwunsch Institut Schenk, Dobl, Austria, and Biobank Graz of the Medical University of Graz, Austria. It can be used as a template for other institutions to unify specimen collection procedures in the field of reproductive health research.

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Title: Biobanking of different body fluids within the frame of IVF—a standard operating procedure to improve reproductive biology research
Authors: Michael Schenk
Berthold Huppertz
Barbara Obermayer-Pietsch
Darja Kastelic
Martina Hörmann-Kröpfl
Gregor Weiss
Publication date: 01-02-2017
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 2/2017
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-016-0847-5

At a glance: The STEP trials

Springer Medicine

Biobanking of different body fluids within the frame of IVF—a standard operating procedure to improve reproductive biology research

Abstract

Purpose

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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