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Clinical Phytoscience

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Open Access 01-12-2017 | Original Contribution

Bioactivities of Bruguiera gymnorrhiza and profiling of its bioactive polyphenols by HPLC-DAD

Authors: Imtiaz Mahmud, Md. Nazmul Hasan Zilani, Nripendra Nath Biswas, Bishwajit Bokshi

Published in: Clinical Phytoscience | Issue 1/2017

Abstract

Background

In folk medicine leaves and stem of Bruguiera gymnorrhiza (L.) are commonly used to treat diarrhea, fever, diabetes, pain and a number of ailments. The present study was carried out to explore antioxidant, analgesic and antidiarrhoeal activities of ethanol extract of leaves and stem of B. gymnorrhiza and also to analyze its major bioactive natural polyphenols by HPLC-DAD.

Methods

Total polyphenol content was spectrophotometrically determined using Folin Chiocalteu’s reagent while the flavonoids by aluminum chloride colorimetric assay. Antioxidant activity was determined by DPPH free radical scavenging, reducing power, nitric oxide and hydrogen peroxide scavenging assays. Identification and quantification of bioactive polyphenols were done by HPLC-DAD method. Antidiarrhoeal activity of the extracts was evaluated using experimentally castor oil induced diarrhea in mice. Acetic acid induced writhing method was used to evaluate the analgesic activity. Acute oral toxicity and brine shrimp lethality assay were performed to check the cytotoxic potential.

Results

Both the leave and stem extracts contain significant amount of phenolic and flvonoid content. Extracts showed DPPH radical scavenging, nitric oxide, hydrogen peroxide scavenging and also concentration dependent reducing power activity. HPLC analysis of both extract indicated the presence of significant amount of vanillic acid along with other phenolic constituents. Both extracts showed significant (P < 0.01) analgesic and antidiarrhoeal activity. Furthermore, extracts showed negligible toxic effect.

Conclusion

Along with other phenolic compounds, vanillic acid present in the extract may be responsible for antioxidant, analgesic and antidiarrhoeal activities. Altogether these results rationalize the use of this plant in traditional medicine.

Phang CW, Malek SN, Ibrahim H. Antioxidant potential, cytotoxic activity and total phenolic content of Alpinia pahangensis rhizomes. BMC Complement Altern Med. 2013;13:243. doi:10.1186/1472-6882-13-243.CrossRefPubMedPubMedCentral

Doss A. Preliminary phytochemical screening of some Indian medicinal plants. Ancient Sci Life. 2009;29(2):12–6.

Balaji G, Chalamaiah M, Ramesh B, Amarnath YR. Antidiarrhoeal activity of ethanol and aqueous extracts of Carum copticum seeds in experimental rats. Asian Pac J Trop Biomed. 2012. doi:10.1016/S2221-1691(12)60376–1.

Coe FG, Anderson GJ. Screening of medicinal plants used by the Gar’ıfuna of eastern Nicaragua for bioactive compounds. J Ethnopharmacol. 1996;53:29–50.CrossRefPubMed

Hassan MM, Khan SA, Shaikat AH, Hossain ME, Hoque MA, Ullah MH, et al. Analgesic and anti-inflammatory effects of ethanol extracted leaves of selected medicinal plants in animal model. Vet World. 2013;6(2):68–71. doi:10.5455/vetworld.2013.68-71.CrossRef

Azam MNK, Rahman MM, Biswas S, Ahmed MN. Appraisals of Bangladeshi medicinal plants used by folk medicine practitioners in the prevention and management of malignant neoplastic diseases. Int Scholarly Res Not. 2016. doi:10.1155/2016/7832120.

Tumpa SI, Hossain MI, Ishika T. Ethnomedicinal uses of herbs by indigenous medicine practitioners of Jhenaidah district, Bangladesh. J Pharmacogn Phytochem. 2014;3(2):23–33.

Shaikh JU, Grice ID, Tiralongo E. Cytotoxic effects of Bangladeshi medicinal plant extracts. Evid Based Complement Alternat Med. 2011. doi:10.1093/ecam/nep111.

Li H, Xueshi H, Sattler I, Dahse HM, Hongzheng F, Grabley S, Wenhan L. Three new pimaren diterpenoids from marine mangrove plant, Bruguiera gymnorrhiza. Pharmazie. 2005;60:705–7. doi:10.1002/chin.200601187.

10.

Li H, Xueshi H, Isabel S, Moellmann U, Hongzheng F, Wenhan L, et al. New aromatic compounds from the marine mangrove Bruguiera gymnorrhiza. Planta Med. 2005;71:160–4. doi:10.1055/s-2005-837784.CrossRef

11.

Xiang-Xi Y, Jia-Gang D, Cheng-Hai G, Xiao-Tao H, Fei L, Zhi-Ping W, Er-Wei H, Yan X, Zheng-Cai D, Hui-Xue H, Ri-Ming H. Four new cyclohexylideneacetonitrile derivatives from the hypocotyl of mangrove (Bruguiera gymnorrhiza). Molecules. 2015;20:14565–75.CrossRef

12.

Ghani A. Practical phytochemistry. Dhaka: Parash Publishers; 2005.

13.

Zilani MNH, Amirul MI, Sharmin SK, Jamil AS, Mustafizur MR, Golam MH. Analgesic and antioxidant activities of Colocasia fallax. Orient Pharm Exp Med. 2016;16:131–7. doi:10.1007/s13596-016-0222-1.CrossRef

14.

Anisuzzman M, Nazmul MHZ, Sharmin SK, Asaduzzman M, Golam MH. Antioxidant, antibacterial potential and HPLC analysis of Dioscorea alata bulb. Indonesian J Pharm. 2016;27(1):9–14.CrossRef

15.

Salma AS, Siraj MA, Hossain A, Mia MS, Afrin S, Rahman MM. Investigation of the key pharmacological activities of Ficu sracemosa and analysis of its major bioactive polyphenols by HPLC-DAD. Evid Based Complement Altern Med. 2016. doi:10.1155/2016/3874516.

16.

Sanjib S, Jamil AS, Himangsu M, Royhan G, Morsaline B, Lutfun N, et al. Bioactivity studies on Musa seminifera Lour. Pharmacogn Mag. 2013;9:315–22. doi:10.4103/0973-1296.117827.CrossRef

17.

Rahmat AK, Muhammad RK, Sumaira S, Mushtaq A. Evaluation of phenolic contents and antioxidant activity of various solvent extracts of Sonchus asper (L.) Hill. Chem Cent J. 2012;6:12.

18.

Usha V, Suriyavathana M. Free radical scavenging activity of ethanolic extract of Desmodium gangeticum. J Acute Med. 2012;2:36–42.CrossRef

19.

Khirul MI, Nripendra NB, Sanjib S, Hemayet H, Ismet AJ, Tanzir AK, et al. Antinociceptive and antioxidant activity of Zanthoxylum budrunga Wall (Rutaceae) Seeds. Sci World J. 2014. doi:10.1155/2014/869537.

20.

Islam MA, Ahmed F, Das AK, Bachar SC. Analgesic and anti-inflammatory activity of Leonurus sibiricus. Fitoterapia. 2005;76:359–62.CrossRefPubMed

21.

Bishwajit B, Nazmul MHZ, Aparajita M, Debendra NR, Jamil AS, Samir KS. Study of analgesic and antidiarrhoeal activities of sonneratia caseolaris (linn.) Leaf and stem using different solvent system. Indonesian J Pharm. 2013;24:255–60.

22.

Abdulwali A, Jamaludin M, Khalijah A, Jamil AS, Aditya A. Evaluation of antidiabetic and antioxidant properties of Brucea javanica seed. Sci World J. 2014. doi:10.1155/2014/786130.

23.

Meyer BN, Ferrigni NR, Putnum JE, Jacobson LB, Nicholos DE, McLaugline JL. Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med. 1982;45:31–4.CrossRef

24.

Kanti BP, Syed IR. Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longev. 2009;2:270–8. doi:10.4161/oxim.2.5.9498.CrossRef

25.

Dejan ZO, Neda MM, Marina MF, Slobodan SP, Emilija ĐJ. Antioxidant activity relationship of phenolic compounds in Hypericum perforatum L. Chem Cent J. 2011;5:34. doi:10.1186/1752-153X-5-34.CrossRef

26.

Bendary E, Francis RR, Ali HMG, Sarwat MI, Hady SE. Antioxidant and structure–activity relationships (SARs) of some phenolic and anilines compounds. Ann Agric Sci. 2013;58(2):173–81.

27.

Rong T. Chemistry and biochemistry of dietary polyphenols. Nutrients. 2010;2:1231–46. doi:10.3390/nu2121231.CrossRef

28.

Małgorzata M, Irena P. Antioxidant activity of the main phenolic compounds isolated from hot pepper fruit (Capsicum annuum L.). J Agric Food Chem. 2005;53:1750–6.CrossRef

29.

Himangsu M, Sanjib S, Khalijah A, Hemayet H, Abdulwali A, Khirul MI, et al. Central-stimulating and analgesic activity of the ethanolic extract of Alternanthera sessilis in mice. BMC Complement Altern Med. 2014;14:398. doi:10.1186/1472-6882-14-398.CrossRef

30.

Dhara AK, Suba V, Sen T, Pal S, Chaudhuri AK. Preliminary studies on the anti-inflammatory and analgesic activity of methanolic fraction of the root of Tragia involucrate. J Ethnopharmacol. 2000;72:265–8.CrossRefPubMed

31.

Shafiur R. Antioxida nt, analgesic, cytotoxic and antidiarrh eal activities of ethanolic Zizyphus mauritiana bark extract. Orient Pharm Exp Med. 2012;12:67–73. doi:10.1007/s13596-011-0042-2.CrossRef

32.

Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: an overview. Sci World J. 2013. doi:10.1155/2013/162750.

33.

Bhaskar VH, Balakrishnan N. Analgesic, anti-infammatory and antipyretic activities of Pergularia daemia and Carissa carandas. DARU J Pharm Sci. 2009;17:168–74.

34.

Cássia C, Thacyana TC, Miriam SNH, Felipe AP, Victor F, Marília FM, et al. Vanillic acid inhibits inflammatory pain by inhibiting neutrophil recruitment, oxidative stress, cytokine production, and NFκB activation in mice. J Nat Prod. 2015;78:1799–08. doi:10.1021/acs.jnatprod.5b00246.CrossRef

35.

Yrbas ML, Morucci F, Alonso R, Gorzalczany S. Pharmacological mechanism underlying the antinociceptive activity of vanillic acid. Pharmacol Biochem Behav. 2015;132:88–95. doi:10.1016/j.pbb.2015.02.016.CrossRefPubMed

36.

Khalilur MR, Soumitra B, Fokhrul MI, Rafikul MI, Sayeed MA, Shahnaj P, et al. Studies on the anti-diarrheal properties of leaf extract of Desmodium puchellum. Asian Pac J Trop Biomed. 2013;3:639–43.CrossRef

37.

Khalilur MR, Ashraf MUC, Taufiqual MI, Anisuzzaman MC, Erfan MU, Chandra DS. Evaluation of antidiarrheal activity of methanolic extract of Maranta arundinacea linn. Leaves. Adv Pharmacol Sci. 2015. doi:10.1155/2015/257057.

38.

Qnais EY, Elokda AS, Abu Ghalyun YY, Abdulla FA. Antidiarrheal activity of the aqueous extract of Punica granatum (Pomegranate) peels. Pharm Biol. 2007;45:715–20.CrossRef

39.

Balaji G, Chalamaiah M, Ramesh B, Amarnath RY. Antidiarrhoeal activity of ethanol and aqueous extracts of Carum copticum seeds in experimental rats. Asian Pac J Trop Biomed. 2012;2(2):S1151-55.

40.

Ibrahim OMS, Shwaysh MM. Evaluation of Punica granatum peels extracts and its phenolic, alkaloid and terpenoid constituents against chemically induced diarrhoea in rats. Adv Anim Vet Sci. 2016;4:161–8. doi:10.14737/journal.aavs/2016/4.3.161.168.CrossRef

41.

Chanchal NR, Balasubramaniam A, Sayyed N. Antidiarrheal potential of Tabernaemontana divaricata. Phytopharmacology. 2013;4:61–8.

42.

Ezeigbo II, Ezeja MI, Madubuike KG, Ifenkwe DC, Ukweni IA, Udeh NE, Akomas SC. Antidiarrhoeal activity of leaf methanolic extract of Rauwolfia serpentine. Asian Pac J Trop Biomed. 2012;2:430–2.CrossRefPubMedPubMedCentral

43.

Ihekwereme CP, Erhirhie EO, Mbagwu IS, Ilodigwe EE, Ajaghaku DL, Okoye FB. Antidiarrheal property of Napoleona imperialis may be due to procyanidins and ellagic acid derivatives. J App Pharm Sci. 2016;6:101–6.CrossRef

44.

Hai-Tao X, Siu-Wai T, Hong-Yan Q, Franky FK, Choia ZY, Quan-Bin H, Hu-Biao C, Hong-Xi X, Hong S, Ai-Ping L, Zhao-Xiang B. A bioactivity-guided study on the anti-diarrheal activity of Polygonum chinense Linn. J Ethnopharmacol. 2013;149:499–505. doi:10.1016/j.jep.2013.07.007.CrossRef

45.

Louise IM, Walker R, Catherine BP, Augustin S. Risks and safety of polyphenol consumption. Am J Clin Nutr. 2005;81:326S–9.

46.

Cerda B, Ceron JJ, Tomas-Barberan FA, Espin JC. Repeated oral administration of high doses of the pomegranate ellagitannin punicalagin to rats for 37 days is not toxic. J Agric Food Chem. 2003;51:3493–501.CrossRefPubMed

47.

Joshua DL, Mary JK, Shengmin S, Kenneth RR, Jihyeung J, Chung SY. Hepatotoxicity of high oral dose (−)-Epigallocatechin-3-Gallate in mice. Food Chem Toxicol. 2010;48(1):409–16. doi:10.1016/j.fct.2009.10.030.CrossRef

48.

Isomura T, Suzuki S, Origasa H, Hosono A, Suzuki M, Sawada T, Terao S, Muto Y, Koga T. Liver-related safety assessment of green tea extracts in humans: a systematic review of randomized controlled trials. Eur J Clin Nutr. 2016;70:1221–9.CrossRefPubMedPubMedCentral

Title: Bioactivities of Bruguiera gymnorrhiza and profiling of its bioactive polyphenols by HPLC-DAD
Authors: Imtiaz Mahmud
Md. Nazmul Hasan Zilani
Nripendra Nath Biswas
Bishwajit Bokshi
Publication date: 01-12-2017
Publisher: Springer Berlin Heidelberg
Published in: Clinical Phytoscience / Issue 1/2017
Electronic ISSN: 2199-1197
DOI: https://doi.org/10.1186/s40816-017-0048-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Bioactivities of Bruguiera gymnorrhiza and profiling of its bioactive polyphenols by HPLC-DAD

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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