Skip to main content
Key Specialties
Anesthesiology Cardiology Dermatology Diabetology Endocrinology Gastroenterology General Medicine Geriatrics Hematology Infectious Diseases Internal Medicine Nephrology Neurology Obstetrics and Gynecology Oncology Ophthalmology Pediatrics Psychiatry Radiology Respiratory Medicine Rheumatology Surgery Urology
Congress Coverage
ASH 2024 Annual Meeting 2024 ESMO Congress 2024 ERS Congress 2024 EASD Congress 2024 ESC Congress 2024 EAN Congress 2024 ASCO Annual Meeting 2024 ACC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on adolescent vaping

LIVE: Wednesday 29th January, 18:00-19:30 CET

Growing numbers of young people are using e-cigarettes, despite warnings of respiratory effects and addiction. How can doctors tackle the epidemic, and what health effects should you prepare to manage in your clinics?
ADVANCED SEARCH
Log in
Top
Clinical Phytoscience
Published in:

Open Access 01-12-2017 | Original Contribution

Bioactivities of Bruguiera gymnorrhiza and profiling of its bioactive polyphenols by HPLC-DAD

Authors: Imtiaz Mahmud, Md. Nazmul Hasan Zilani, Nripendra Nath Biswas, Bishwajit Bokshi

Published in: Clinical Phytoscience | Issue 1/2017

Login to get access

Abstract

Background

In folk medicine leaves and stem of Bruguiera gymnorrhiza (L.) are commonly used to treat diarrhea, fever, diabetes, pain and a number of ailments. The present study was carried out to explore antioxidant, analgesic and antidiarrhoeal activities of ethanol extract of leaves and stem of B. gymnorrhiza and also to analyze its major bioactive natural polyphenols by HPLC-DAD.

Methods

Total polyphenol content was spectrophotometrically determined using Folin Chiocalteu’s reagent while the flavonoids by aluminum chloride colorimetric assay. Antioxidant activity was determined by DPPH free radical scavenging, reducing power, nitric oxide and hydrogen peroxide scavenging assays. Identification and quantification of bioactive polyphenols were done by HPLC-DAD method. Antidiarrhoeal activity of the extracts was evaluated using experimentally castor oil induced diarrhea in mice. Acetic acid induced writhing method was used to evaluate the analgesic activity. Acute oral toxicity and brine shrimp lethality assay were performed to check the cytotoxic potential.

Results

Both the leave and stem extracts contain significant amount of phenolic and flvonoid content. Extracts showed DPPH radical scavenging, nitric oxide, hydrogen peroxide scavenging and also concentration dependent reducing power activity. HPLC analysis of both extract indicated the presence of significant amount of vanillic acid along with other phenolic constituents. Both extracts showed significant (P < 0.01) analgesic and antidiarrhoeal activity. Furthermore, extracts showed negligible toxic effect.

Conclusion

Along with other phenolic compounds, vanillic acid present in the extract may be responsible for antioxidant, analgesic and antidiarrhoeal activities. Altogether these results rationalize the use of this plant in traditional medicine.
Literature
1.
2.
Doss A. Preliminary phytochemical screening of some Indian medicinal plants. Ancient Sci Life. 2009;29(2):12–6.
3.
Balaji G, Chalamaiah M, Ramesh B, Amarnath YR. Antidiarrhoeal activity of ethanol and aqueous extracts of Carum copticum seeds in experimental rats. Asian Pac J Trop Biomed. 2012. doi:10.​1016/​S2221-1691(12)60376–1.
4.
Coe FG, Anderson GJ. Screening of medicinal plants used by the Gar’ıfuna of eastern Nicaragua for bioactive compounds. J Ethnopharmacol. 1996;53:29–50.CrossRefPubMed
5.
Hassan MM, Khan SA, Shaikat AH, Hossain ME, Hoque MA, Ullah MH, et al. Analgesic and anti-inflammatory effects of ethanol extracted leaves of selected medicinal plants in animal model. Vet World. 2013;6(2):68–71. doi:10.​5455/​vetworld.​2013.​68-71.CrossRef
6.
Azam MNK, Rahman MM, Biswas S, Ahmed MN. Appraisals of Bangladeshi medicinal plants used by folk medicine practitioners in the prevention and management of malignant neoplastic diseases. Int Scholarly Res Not. 2016. doi:10.​1155/​2016/​7832120.
7.
Tumpa SI, Hossain MI, Ishika T. Ethnomedicinal uses of herbs by indigenous medicine practitioners of Jhenaidah district, Bangladesh. J Pharmacogn Phytochem. 2014;3(2):23–33.
8.
Shaikh JU, Grice ID, Tiralongo E. Cytotoxic effects of Bangladeshi medicinal plant extracts. Evid Based Complement Alternat Med. 2011. doi:10.​1093/​ecam/​nep111.
9.
Li H, Xueshi H, Sattler I, Dahse HM, Hongzheng F, Grabley S, Wenhan L. Three new pimaren diterpenoids from marine mangrove plant, Bruguiera gymnorrhiza. Pharmazie. 2005;60:705–7. doi:10.​1002/​chin.​200601187.
10.
Li H, Xueshi H, Isabel S, Moellmann U, Hongzheng F, Wenhan L, et al. New aromatic compounds from the marine mangrove Bruguiera gymnorrhiza. Planta Med. 2005;71:160–4. doi:10.​1055/​s-2005-837784.CrossRef
11.
Xiang-Xi Y, Jia-Gang D, Cheng-Hai G, Xiao-Tao H, Fei L, Zhi-Ping W, Er-Wei H, Yan X, Zheng-Cai D, Hui-Xue H, Ri-Ming H. Four new cyclohexylideneacetonitrile derivatives from the hypocotyl of mangrove (Bruguiera gymnorrhiza). Molecules. 2015;20:14565–75.CrossRef
12.
Ghani A. Practical phytochemistry. Dhaka: Parash Publishers; 2005.
13.
14.
Anisuzzman M, Nazmul MHZ, Sharmin SK, Asaduzzman M, Golam MH. Antioxidant, antibacterial potential and HPLC analysis of Dioscorea alata bulb. Indonesian J Pharm. 2016;27(1):9–14.CrossRef
15.
Salma AS, Siraj MA, Hossain A, Mia MS, Afrin S, Rahman MM. Investigation of the key pharmacological activities of Ficu sracemosa and analysis of its major bioactive polyphenols by HPLC-DAD. Evid Based Complement Altern Med. 2016. doi:10.​1155/​2016/​3874516.
16.
17.
Rahmat AK, Muhammad RK, Sumaira S, Mushtaq A. Evaluation of phenolic contents and antioxidant activity of various solvent extracts of Sonchus asper (L.) Hill. Chem Cent J. 2012;6:12.
18.
Usha V, Suriyavathana M. Free radical scavenging activity of ethanolic extract of Desmodium gangeticum. J Acute Med. 2012;2:36–42.CrossRef
19.
Khirul MI, Nripendra NB, Sanjib S, Hemayet H, Ismet AJ, Tanzir AK, et al. Antinociceptive and antioxidant activity of Zanthoxylum budrunga Wall (Rutaceae) Seeds. Sci World J. 2014. doi:10.​1155/​2014/​869537.
20.
Islam MA, Ahmed F, Das AK, Bachar SC. Analgesic and anti-inflammatory activity of Leonurus sibiricus. Fitoterapia. 2005;76:359–62.CrossRefPubMed
21.
Bishwajit B, Nazmul MHZ, Aparajita M, Debendra NR, Jamil AS, Samir KS. Study of analgesic and antidiarrhoeal activities of sonneratia caseolaris (linn.) Leaf and stem using different solvent system. Indonesian J Pharm. 2013;24:255–60.
22.
Abdulwali A, Jamaludin M, Khalijah A, Jamil AS, Aditya A. Evaluation of antidiabetic and antioxidant properties of Brucea javanica seed. Sci World J. 2014. doi:10.​1155/​2014/​786130.
23.
Meyer BN, Ferrigni NR, Putnum JE, Jacobson LB, Nicholos DE, McLaugline JL. Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med. 1982;45:31–4.CrossRef
24.
25.
26.
Bendary E, Francis RR, Ali HMG, Sarwat MI, Hady SE. Antioxidant and structure–activity relationships (SARs) of some phenolic and anilines compounds. Ann Agric Sci. 2013;58(2):173–81.
27.
28.
Małgorzata M, Irena P. Antioxidant activity of the main phenolic compounds isolated from hot pepper fruit (Capsicum annuum L.). J Agric Food Chem. 2005;53:1750–6.CrossRef
29.
Himangsu M, Sanjib S, Khalijah A, Hemayet H, Abdulwali A, Khirul MI, et al. Central-stimulating and analgesic activity of the ethanolic extract of Alternanthera sessilis in mice. BMC Complement Altern Med. 2014;14:398. doi:10.​1186/​1472-6882-14-398.CrossRef
30.
Dhara AK, Suba V, Sen T, Pal S, Chaudhuri AK. Preliminary studies on the anti-inflammatory and analgesic activity of methanolic fraction of the root of Tragia involucrate. J Ethnopharmacol. 2000;72:265–8.CrossRefPubMed
31.
32.
33.
Bhaskar VH, Balakrishnan N. Analgesic, anti-infammatory and antipyretic activities of Pergularia daemia and Carissa carandas. DARU J Pharm Sci. 2009;17:168–74.
34.
Cássia C, Thacyana TC, Miriam SNH, Felipe AP, Victor F, Marília FM, et al. Vanillic acid inhibits inflammatory pain by inhibiting neutrophil recruitment, oxidative stress, cytokine production, and NFκB activation in mice. J Nat Prod. 2015;78:1799–08. doi:10.​1021/​acs.​jnatprod.​5b00246.CrossRef
35.
36.
Khalilur MR, Soumitra B, Fokhrul MI, Rafikul MI, Sayeed MA, Shahnaj P, et al. Studies on the anti-diarrheal properties of leaf extract of Desmodium puchellum. Asian Pac J Trop Biomed. 2013;3:639–43.CrossRef
37.
Khalilur MR, Ashraf MUC, Taufiqual MI, Anisuzzaman MC, Erfan MU, Chandra DS. Evaluation of antidiarrheal activity of methanolic extract of Maranta arundinacea linn. Leaves. Adv Pharmacol Sci. 2015. doi:10.​1155/​2015/​257057.
38.
Qnais EY, Elokda AS, Abu Ghalyun YY, Abdulla FA. Antidiarrheal activity of the aqueous extract of Punica granatum (Pomegranate) peels. Pharm Biol. 2007;45:715–20.CrossRef
39.
Balaji G, Chalamaiah M, Ramesh B, Amarnath RY. Antidiarrhoeal activity of ethanol and aqueous extracts of Carum copticum seeds in experimental rats. Asian Pac J Trop Biomed. 2012;2(2):S1151-55.
40.
41.
Chanchal NR, Balasubramaniam A, Sayyed N. Antidiarrheal potential of Tabernaemontana divaricata. Phytopharmacology. 2013;4:61–8.
42.
Ezeigbo II, Ezeja MI, Madubuike KG, Ifenkwe DC, Ukweni IA, Udeh NE, Akomas SC. Antidiarrhoeal activity of leaf methanolic extract of Rauwolfia serpentine. Asian Pac J Trop Biomed. 2012;2:430–2.CrossRefPubMedPubMedCentral
43.
Ihekwereme CP, Erhirhie EO, Mbagwu IS, Ilodigwe EE, Ajaghaku DL, Okoye FB. Antidiarrheal property of Napoleona imperialis may be due to procyanidins and ellagic acid derivatives. J App Pharm Sci. 2016;6:101–6.CrossRef
44.
Hai-Tao X, Siu-Wai T, Hong-Yan Q, Franky FK, Choia ZY, Quan-Bin H, Hu-Biao C, Hong-Xi X, Hong S, Ai-Ping L, Zhao-Xiang B. A bioactivity-guided study on the anti-diarrheal activity of Polygonum chinense Linn. J Ethnopharmacol. 2013;149:499–505. doi:10.​1016/​j.​jep.​2013.​07.​007.CrossRef
45.
Louise IM, Walker R, Catherine BP, Augustin S. Risks and safety of polyphenol consumption. Am J Clin Nutr. 2005;81:326S–9.
46.
Cerda B, Ceron JJ, Tomas-Barberan FA, Espin JC. Repeated oral administration of high doses of the pomegranate ellagitannin punicalagin to rats for 37 days is not toxic. J Agric Food Chem. 2003;51:3493–501.CrossRefPubMed
47.
48.
Isomura T, Suzuki S, Origasa H, Hosono A, Suzuki M, Sawada T, Terao S, Muto Y, Koga T. Liver-related safety assessment of green tea extracts in humans: a systematic review of randomized controlled trials. Eur J Clin Nutr. 2016;70:1221–9.CrossRefPubMedPubMedCentral
Metadata
Title
Bioactivities of Bruguiera gymnorrhiza and profiling of its bioactive polyphenols by HPLC-DAD
Authors
Imtiaz Mahmud
Md. Nazmul Hasan Zilani
Nripendra Nath Biswas
Bishwajit Bokshi
Publication date
01-12-2017
Publisher
Springer Berlin Heidelberg
Published in
Clinical Phytoscience / Issue 1/2017
Electronic ISSN: 2199-1197
DOI
https://doi.org/10.1186/s40816-017-0048-5

Other articles of this Issue 1/2017

Aqueous extract of Dennettia tripetala ameliorates liver and kidney damage caused by multiple exposures to carbon tetrachloride

  • Open Access
  • Original contribution

Mitigative effects of Moringa oleifera against liver injury induced by artesunate-amodiaquine antimalarial combination in wistar rats

  • Open Access
  • Original Contribution

Neuroprotective effects of Peltophorum pterocarpum leaf extract against hydrogen peroxide induced oxidative stress and cytotoxicity

  • Open Access
  • Original contribution

Modulation of oxidative stress mediators in the liver of adjuvant induced arthritic rats by Nyctanthes arbor tristis

  • Open Access
  • Original contribution

Toxicological implications of the therapeutic use of Acalypha wilkesiana leaves in traditional medicine

  • Open Access
  • Original contribution

Biological activity of Bauhinia racemosa against Diabetes and Interlinked Disorders like Obesity and Hyperlipidemia

  • Open Access
  • Original contribution