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Critical Care
Published in: Critical Care 3/2012

01-06-2012 | Review

Bench-to-bedside review: Rapid molecular diagnostics for bloodstream infection - a new frontier?

Authors: Arash Afshari, Jacques Schrenzel, Margareta Ieven, Stephan Harbarth

Published in: Critical Care | Issue 3/2012

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Abstract

Among critically ill patients, the diagnosis of bloodstream infection poses a major challenge. Current standard bacterial identification based on blood culture platforms is intrinsically time-consuming and slow. The continuous evolvement of molecular techniques has the potential of providing a faster, more sensitive and direct identification of causative pathogens without prior need for cultivation. This may ultimately impact clinical decision-making and antimicrobial treatment. This review summarises the currently available technologies, their strengths and limitations and the obstacles that have to be overcome in order to develop a satisfactory bedside point-of-care diagnostic tool for detection of bloodstream infection.
Literature
1.
Melamed A, Sorvillo FJ: The burden of sepsis-associated mortality in the United States from 1999 to 2005: an analysis of multiplecause-of-death data. Crit Care. 2009, 13: R28-10.1186/cc7733.PubMedCentralPubMed
2.
Engel C, Brunkhorst FM, Bone HG, Brunkhorst R, Gerlach H, Grond S, Gruendling M, Huhle G, Jaschinski U, John S, Mayer K, Oppert M, Olthoff D, Quintel M, Ragaller M, Rossaint R, Stuber F, Weiler N, Welte T, Bogatsch H, Hartog C, Loeffler M, Reinhart K: Epidemiology of sepsis in Germany: results from a national prospective multicenter study. Intensive Care Med. 2007, 33: 606-618. 10.1007/s00134-006-0517-7.PubMed
3.
Harbarth S, Garbino J, Pugin J, Romand J, Lew D, Pittet D: Inappropriate initial antimicrobial therapy and its effect on survival in a clinical trial of immunomodulating therapy for severe sepsis. Am J Med. 2003, 115: 529-535. 10.1016/j.amjmed.2003.07.005.PubMed
4.
Kumar A, Ellis P, Arabi Y, Roberts D, Light B, Parrillo JE, Dodek P, Wood G, Kumar A, Simon D, Peters C, Ahsan M, Chateau D, Cooperative Antimicrobial Therapy of Septic Shock Database Research Group: Initiation of inappropriate antimicrobial therapy results in a fivefold reduction of survival in human septic shock. Chest. 2009, 136: 1237-1248. 10.1378/chest.09-0087.PubMed
5.
Carlet J, Collignon P, Goldmann D, Goossens H, Gyssens IC, Harbarth S, Jarlier V, Levy SB, N'Doye B, Pittet D, Richtmann R, Seto WH, van der Meer JW, Voss A: Society's failure to protect a precious resource: antibiotics. Lancet. 2011, 378: 369-371. 10.1016/S0140-6736(11)60401-7.PubMed
6.
Binder U, Lass-Flörl C: Epidemiology of invasive fungal infections in the mediterranean area. Mediterr J Hematol Infect Dis. 2011, 3: e20110016-PubMedCentralPubMed
7.
Miceli MH, Lee SA: Emerging moulds: epidemiological trends and antifungal resistance. Mycoses. 2011, 54: e666-678. 10.1111/j.1439-0507.2011.02032.x.PubMed
8.
Bassetti M, Ginocchio F, Giacobbe DR: New approaches for empiric therapy in Gram-positive sepsis. Minerva Anestesiol. 2011, 77: 821-827.PubMed
9.
Spapen H, Jacobs R, Van Gorp V, Troubleyn J, Honoré PM: Renal and neurological side effects of colistin in critically ill patients. Ann Intensive Care. 2011, 1: 14-10.1186/2110-5820-1-14.PubMedCentralPubMed
10.
Kane-Gill SL, Jacobi J, Rothschild JM: Adverse drug events in intensive care units: risk factors, impact, and the role of team care. Crit Care Med. 2010, 38: S83-89.PubMed
11.
Vallés J, Rello J, Ochagavía A, Garnacho J, Alcalá MA: Community-acquired bloodstream infection in critically ill adult patients: impact of shock and inappropriate antibiotic therapy on survival. Chest. 2003, 123: 1615-1624.PubMed
12.
Garnacho-Montero J, Ortiz-Leyba C, Herrera-Melero I, Aldabó-Pallás T, Cayuela-Dominguez A, Marquez-Vacaro JA, Carbajal-Guerrero J, Garcia-Garmendia JL: Mortality and morbidity attributable to inadequate empirical antimicrobial therapy in patients admitted to the ICU with sepsis: a matched cohort study. J Antimicrob Chemother. 2008, 61: 436-441.PubMed
13.
Juan-Torres A, Harbarth S: Prevention of primary bacteraemia. Int J Antimicrob Agents. 2007, S80-87. Suppl 1
14.
Seifert H: The clinical importance of microbiological findings in the diagnosis and management of bloodstream infections. Clin Infect Dis. 2009, 48: S238-S245. 10.1086/598188.PubMed
15.
Mancini N, Carletti S, Ghidoli N, Cichero P, Burioni R, Clementi M: The era of molecular and other non-culture-based methods in diagnosis of sepsis. Clin Microbiol Rev. 2010, 23: 235-251. 10.1128/CMR.00043-09.PubMedCentralPubMed
16.
Fenollar F, Raoult D: Molecular diagnosis of bloodstream infections caused by non-cultivable bacteria. Int J Antimicrob Agents. 2007, 30: S7-S15.PubMed
17.
Raad I, Hanna H, Maki D: Intravascular catheter-related infections: advances in diagnosis, prevention, and management. Lancet Infect Dis. 2007, 7: 645-657. 10.1016/S1473-3099(07)70235-9.PubMed
18.
Lamas CC, Eykyn SJ: Blood culture negative endocarditis: analysis of 63 cases presenting over 25 years. Heart. 2003, 89: 258-262. 10.1136/heart.89.3.258.PubMedCentralPubMed
19.
Lee A, Mirrett S, Reller LB, Weinstein MP: Detection of bloodstream infections in adults: how many blood cultures are needed?. J Clin Microbiol. 2007, 45: 3546-3548. 10.1128/JCM.01555-07.PubMedCentralPubMed
20.
Connell TG, Rele M, Cowley D, Buttery JP, Curtis N: How reliable is a negative blood culture result? Volume of blood submitted for culture in routine practice in a children's hospital. Pediatrics. 2007, 119: 891-896. 10.1542/peds.2006-0440.PubMed
21.
22.
Leggieri N, Rida A, François P, Schrenzel J: Molecular diagnosis of bloodstream infections: planning to (physically) reach the bedside. Curr Opin Infect Dis. 2010, 23: 311-319.PubMed
23.
Shah SS, Downes KJ, Elliott MR, Bell LM, McGowan KL, Metlay JP: How long does it take to ''rule out'' bacteremia in children with central venous catheters?. Pediatrics. 2008, 121: 135-141. 10.1542/peds.2007-1387.PubMed
24.
Peralta G, Rodriguez-Lera MJ, Garrido JC, Ansorena L, Roiz MP: Time to positivity in blood cultures of adults with Streptococcus pneumoniae bacteremia. BMC Infect Dis. 2006, 6: 79-10.1186/1471-2334-6-79.PubMedCentralPubMed
25.
Okeke IN, Peeling RW, Goossens H, Auckenthaler R, Olmsted SS, de Lavison JF, Zimmer BL, Perkins MD, Nordqvist K: Diagnostics as essential tools for containing antibacterial resistance. Drug Resist Updat. 2011, 14: 95-106. 10.1016/j.drup.2011.02.002.PubMed
26.
Wolk DM, Struelens MJ, Pancholi P, Davis T, Della-Latta P, Fuller D, Picton E, Dickenson R, Denis O, Johnson D, Chapin K: Rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in wound specimens and blood cultures: multicenter preclinical evaluation of the Cepheid Xpert MRSA/SA skin and soft tissue and blood culture assays. J Clin Microbiol. 2009, 47: 823-826. 10.1128/JCM.01884-08.PubMedCentralPubMed
27.
Bauer KA, West JE, Balada-Llasat JM, Pancholi P, Stevenson KB, Goff DA: An antimicrobial stewardship program's impact with rapid polymerase chain reaction methicillin-resistant Staphylococcus aureus/S. aureus blood culture test in patients with S. aureus bacteremia. Clin Infect Dis. 2010, 51: 1074-1080. 10.1086/656623.PubMed
28.
Parta M, Goebel M, Thomas J, Matloobi M, Stager C, Musher DM: Impact of an assay that enables rapid determination of Staphylococcus species and their drug susceptibility on the treatment of patients with positive blood culture results. Infect Control Hosp Epidemiol. 2010, 31: 1043-1048. 10.1086/656248.PubMed
29.
Costa AM, Kay I, Palladino S: Rapid detection of mecA and nuc genes in staphylococci by real-time multiplex polymerase chain reaction. Diagn Microbiol Infect Dis. 2005, 51: 13-17. 10.1016/j.diagmicrobio.2004.08.014.PubMed
30.
Stamper PD, Cai M, Howard T, Speser S, Carroll KC: Clinical validation of the molecular BD GeneOhm StaphSR assay for direct detection of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus in positive blood cultures. J Clin Microbiol. 2007, 45: 2191-2196. 10.1128/JCM.00552-07.PubMedCentralPubMed
31.
Ho TH, Huang YC, Lin TY: Evaluation of the BD GeneOhm StaphSR assay for detection of Staphylococcus aureus in patients in intensive care units. J Microbiol Immunol Infect. 2011, 44: 310-315. 10.1016/j.jmii.2010.08.008.PubMed
32.
Gröbner S, Dion M, Plante M, Kempf VA: Evaluation of the BD GeneOhm StaphSR assay for detection of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from spiked positive blood culture bottles. J Clin Microbiol. 2009, 47: 1689-1694. 10.1128/JCM.02179-08.PubMedCentralPubMed
33.
Snyder JW, Munier GK, Heckman SA, Camp P, Overman TL: Failure of the BD GeneOhm StaphSR assay for direct detection of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates in positive blood cultures collected in the United States. J Clin Microbiol. 2009, 47: 3747-3748. 10.1128/JCM.01391-09.PubMedCentralPubMed
34.
Tissari P, Zumla A, Tarkka E, Mero S, Savolainen L, Vaara M, Aittakorpi A, Laakso S, Lindfors M, Piiparinen H, Mäki M, Carder C, Huggett J, Gant V: Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study. Lancet. 2010, 375: 224-230. 10.1016/S0140-6736(09)61569-5.PubMed
35.
Ritchie Alistair, Castán Pablo: Aplicación de nuevas técnicas de microsecuenciación dirigida a la detección de bacterias mediante pyrosecuenciación. LifeScienceLab. 2010, 1: 2-3.
36.
Wellinghausen N, Wirths B, Essig A, Wassill L: Evaluation of the Hyplex BloodScreen multiplex PCR-enzyme-linked immunosorbent assay system for direct identification of gram-positive cocci and gram-negative bacilli from positive blood cultures. J Clin Microbiol. 2004, 42: 3147-3152. 10.1128/JCM.42.7.3147-3152.2004.PubMedCentralPubMed
37.
Avlami A, Bekris S, Ganteris G, Kraniotaki E, Malamou-Lada E, Orfanidou M, Paniara O, Pantazatou A, Papagiannitsis CC, Platsouka E, Stefanou I, Tzelepi E, Vagiakou H, Miriagou V: Detection of metallo-β-lactamase genes in clinical specimens by a commercial multiplex PCR system. J Microbiol Methods. 2010, 83: 185-187. 10.1016/j.mimet.2010.08.014.PubMed
38.
Zhang K, McClure JA, Elsayed S, Louie T, Conly JM: Novel multiplex PCR assay for simultaneous identification of community-associated methicillin resistant Staphylococcus aureus strains USA300 and USA400 and detection of mecA and Panton-Valentine leukocidin genes, with discrimination of Staphylococcus aureus from coagulase-negative staphylococci. J Clin Microbiol. 2008, 46: 1118-1122. 10.1128/JCM.01309-07.PubMedCentralPubMed
39.
Tang YW, Kilic A, Yang Q, McAllister SK, Li H, Miller RS, McCormac M, Tracy KD, Stratton CW, Han J, Limbago B: StaphPlex system for rapid and simultaneous identification of antibiotic resistance determinants and Panton-Valentine leukocidin detection of staphylococci from positive blood cultures. J Clin Microbiol. 2007, 45: 1867-1873. 10.1128/JCM.02100-06.PubMedCentralPubMed
40.
41.
Forrest GN: PNA FISH: present and future impact on patient management. Expert Rev Mol Diagn. 2007, 7: 231-236. 10.1586/14737159.7.3.231.PubMed
42.
Peleg AY, Tilahun Y, Fiandaca MJ, D'Agata EM, Venkataraman L, Moellering RC, Eliopoulos GM: Utility of peptide nucleic acid fluorescence in situ hybridization for rapid detection of Acinetobacter spp. and Pseudomonas aeruginosa. J Clin Microbiol. 2009, 47: 830-832. 10.1128/JCM.01724-08.PubMedCentralPubMed
43.
Forrest Forrest GN, Roghmann MC, Toombs LS, Johnson JK, Weekes E, Lincalis DP, Venezia RA: Peptide nucleic acid fluorescent in situ hybridization for hospital-acquired enterococcal bacteremia: delivering earlier effective antimicrobial therapy. Antimicrob Agents Chemother. 2008, 52: 3558-3563. 10.1128/AAC.00283-08.PubMed
44.
Ly T, Gulia J, Pyrgos V, Waga M, Shoham S: Impact upon clinical outcomes of translation of PNA FISH-generated laboratory data from the clinical microbiology bench to bedside in real time. Ther Clin Risk Manag. 2008, 4: 637-640.PubMedCentralPubMed
45.
Holtzman C, Whitney D, Barlam T, Miller NS: Assessment of impact of peptide nucleic acid fluorescence in situ hybridization for rapid identification of coagulase-negative staphylococci in the absence of antimicrobial stewardship intervention. J Clin Microbiol. 2011, 49: 1581-1582. 10.1128/JCM.02461-10.PubMedCentralPubMed
46.
Shepard JR, Addison RM, Alexander BD, Della-Latta P, Gherna M, Haase G, Hall G, Johnson JK, Merz WG, Peltroche-Llacsahuanga H, Stender H, Venezia RA, Wilson D, Procop GW, Wu F, Fiandaca MJ: Multicenter evaluation of the Candida albicans/Candida glabrata peptide nucleic acid fluorescent in situ hybridization method for simultaneous dual-color identification of C. albicans and C. glabrata directly from blood culture bottles. J Clin Microbiol. 2008, 46: 50-55. 10.1128/JCM.01385-07.PubMedCentralPubMed
47.
La Scola B: Intact cell MALDI-TOF mass spectrometry-based approaches for the diagnosis of bloodstream infections. Expert Rev Mol Diagn. 2011, 11: 287-298.PubMed
48.
Kok J, Thomas LC, Olma T, Chen SC, Iredell JR: Identification of bacteria in blood culture broths using matrix-assisted laser desorption-ionization Sepsityper™ and time of flight mass spectrometry. PLoS One. 2011, 6: e23285-10.1371/journal.pone.0023285.PubMedCentralPubMed
49.
Seng P, Rolain JM, Fournier PE, La Scola B, Drancourt M, Raoult D: MALDI-TOF-mass spectrometry applications in clinical microbiology. Future Microbiol. 2010, 5: 1733-1754. 10.2217/fmb.10.127.PubMed
50.
Drancourt M: Detection of microorganisms in blood specimens using matrix-assisted laser desorption ionization time-of-flight mass spectrometry: a review. Clin Microbiol Infect. 2010, 16: 1620-1625. 10.1111/j.1469-0691.2010.03290.x.PubMed
51.
Cherkaoui A, Hibbs J, Emonet S, Tangomo M, Girard M, Francois P, Schrenzel J: Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level. J Clin Microbiol. 2010, 48: 1169-1175. 10.1128/JCM.01881-09.PubMedCentralPubMed
52.
Sanchez-Jimenez MM, Cardona-Castro N: Validation of a PCR for diagnosis of typhoid fever and salmonellosis by amplification of the hilA gene in clinical samples from Colombian patients. J Med Microbiol. 2004, 53: 875-878. 10.1099/jmm.0.45630-0.PubMed
53.
Rebollo MJ, San Juan Garrido R, Folgueira D, Palenque E, Díaz-Pedroche C, Lumbreras C, Aguado JM: Blood and urine samples as useful sources for the direct detection of tuberculosis by polymerase chain reaction. Diagn Microbiol Infect Dis. 2006, 56: 141-146. 10.1016/j.diagmicrobio.2006.03.018.PubMed
54.
Peters RP, van Agtmael MA, Gierveld S, Danner SA, Groeneveld AB, Vandenbroucke-Grauls CM, Savelkoul PH: Quantitative detection of Staphylococcus aureus and Enterococcus faecalis DNA in blood to diagnose bacteremia in patients in the intensive care unit. J Clin Microbiol. 2007, 45: 3641-3646. 10.1128/JCM.01056-07.PubMedCentralPubMed
55.
Klingspor L, Loeffler J: Aspergillus PCR formidable challenges and progress. Med Mycol. 2009, 47: S241-S247. 10.1080/13693780802616823.PubMed
56.
Mühl H, Kochem AJ, Disqué C, Sakka SG: Activity and DNA contamination of commercial polymerase chain reaction reagents for the universal 16S rDNA real-time polymerase chain reaction detection of bacterial pathogens in blood. Diagn Microbiol Infect Dis. 2010, 66: 41-49. 10.1016/j.diagmicrobio.2008.07.011.PubMed
57.
Wellinghausen N, Kochem AJ, Disqué C, Mühl H, Gebert S, Winter J, Matten J, Sakka SG: Diagnosis of bacteremia in whole-blood samples by use of a commercial universal 16S rRNA gene-based PCR and sequence analysis. J Clin Microbiol. 2009, 47: 2759-2765. 10.1128/JCM.00567-09.PubMedCentralPubMed
58.
Gebert S, Siegel D, Wellinghausen N: Rapid detection of pathogens in blood culture bottles by real-time PCR in conjunction with the pre-analytic tool MolYsis. J Infect. 2008, 57: 307-316. 10.1016/j.jinf.2008.07.013.PubMed
59.
Mencacci A, Leli C, Cardaccia A, Montagna P, Moretti A, Bietolini C, Meucci M, Perito S, Cenci E, Bistoni F: Comparison of conventional culture with SeptiFast real-time PCR for microbial pathogen detection in clinical specimens other than blood. J Med Microbiol. 2011, 60: 1774-1778. 10.1099/jmm.0.034280-0.PubMed
60.
Sancho-Tello S, Bravo D, Borrás R, Costa E, Muñoz-Cobo B, Navarro D: Performance of the LightCycler SeptiFast test Mgrade in detecting microbial pathogens in purulent fluids. J Clin Microbiol. 2011, 49: 2988-2991. 10.1128/JCM.00359-11.PubMedCentralPubMed
61.
Fernández AL, Varela E, Martínez L, Martínez A, Sierra J, González-Juanatey JR, Regueiro B: Evaluation of a multiplex real-time PCR assay for detecting pathogens in cardiac valve tissue in patients with endocarditis. Rev Esp Cardiol. 2010, 63: 1205-1208.PubMed
62.
Lehmann LE, Hunfeld KP, Emrich T, Haberhausen G, Wissing H, Hoeft A, Stüber F: A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples. Med Microbiol Immunol. 2008, 197: 313-324. 10.1007/s00430-007-0063-0.PubMed
63.
Wallet F, Loïez C, Herwegh S, Courcol RJ: Usefulness of real-time PCR for the diagnosis of sepsis in ICU-acquired infections. Infect Disord Drug Targets. 2011, 11: 348-353.PubMed
64.
Bravo D, Blanquer J, Tormo M, Aguilar G, Borrás R, Solano C, Clari MA, Costa E, Muñoz-Cobo B, Argüeso M, Pineda JR, Navarro D: Diagnostic accuracy and potential clinical value of the LightCycler SeptiFast assay in the management of bloodstream infections occurring in neutropenic and critically ill patients. Int J Infect Dis. 2011, 15: e326-331. 10.1016/j.ijid.2011.01.003.PubMed
65.
Josefson P, Strålin K, Ohlin A, Ennefors T, Dragsten B, Andersson L, Fredlund H, Mölling P, Olcén P: Evaluation of a commercial multiplex PCR test (SeptiFast) in the etiological diagnosis of community-onset bloodstream infections. Eur J Clin Microbiol Infect Dis. 2011, 30: 1127-1134. 10.1007/s10096-011-1201-6.PubMed
66.
Lehmann LE, Hauser S, Malinka T, Klaschik S, Weber SU, Schewe JC, Stüber F, Book M: Rapid qualitative urinary tract infection pathogen identification by SeptiFast real-time PCR. PLoS One. 2011, 6: e17146-10.1371/journal.pone.0017146.PubMedCentralPubMed
67.
Figueroa JR, Ortiz J, Morales I: Use of the LightCycler SeptiFast test for rapid etiologic diagnosis of nosocomial infection in gynecological sepsis. Gynecol Obstet Invest. 2010, 70: 215-216. 10.1159/000318868.PubMed
68.
Maubon D, Hamidfar-Roy R, Courby S, Vesin A, Maurin M, Pavese P, Ravanel N, Bulabois CE, Brion JP, Pelloux H, Timsit JF: Therapeutic impact and diagnostic performance of multiplex PCR in patients with malignancies and suspected sepsis. J Infect. 2010, 61: 335-342. 10.1016/j.jinf.2010.07.004.PubMed
69.
Avolio M, Diamante P, Zamparo S, Modolo ML, Grosso S, Zigante P, Tosoni N, De Rosa R, Stano P, Camporese A: Molecular identification of bloodstream pathogens in patients presenting to the emergency department with suspected sepsis. Shock. 2010, 34: 27-30. 10.1097/SHK.0b013e3181e7e80c.PubMed
70.
Paolucci M, Capretti MG, Dal Monte P, Corvaglia L, Landini MP, Varani S, Pession A, Faldella G, Sambri V: Laboratory diagnosis of late-onset sepsis in newborns by multiplex real-time PCR. J Med Microbiol. 2009, 58: 533-534. 10.1099/jmm.0.003848-0.PubMed
71.
Lucignano B, Ranno S, Liesenfeld O, Pizzorno B, Putignani L, Bernaschi P, Menichella D: Multiplex PCR allows rapid and accurate diagnosis of bloodstream infections in newborns and children with suspected sepsis. J Clin Microbiol. 2011, 49: 2252-2258. 10.1128/JCM.02460-10.PubMedCentralPubMed
72.
Tsalik EL, Jones D, Nicholson B, Waring L, Liesenfeld O, Park LP, Glickman SW, Caram LB, Langley RJ, van Velkinburgh JC, Cairns CB, Rivers EP, Otero RM, Kingsmore SF, Lalani T, Fowler VG, Woods CW: Multiplex PCR to diagnose bloodstream infections in patients admitted from the emergency department with sepsis. J Clin Microbiol. 2010, 48: 26-33. 10.1128/JCM.01447-09.PubMedCentralPubMed
73.
Yanagihara K, Kitagawa Y, Tomonaga M, Tsukasaki K, Kohno S, Seki M, Sugimoto H, Shimazu T, Tasaki O, Matsushima A, Ikeda Y, Okamoto S, Aikawa N, Hori S, Obara H, Ishizaka A, Hasegawa N, Takeda J, Kamihira S, Sugahara K, Asari S, Murata M, Kobayashi Y, Ginba H, Sumiyama Y, Kitajima M: Evaluationof pathogen detection from clinical samples by real-time polymerase chain reaction using a sepsis pathogen DNA detection kit. Crit Care. 2010, 14: R159-10.1186/cc9234.PubMedCentralPubMed
74.
Sachse S, Straube E, Lehmann M, Bauer M, Russwurm S, Schmidt KH: Truncated human cytidylate-phosphate-deoxyguanylate-binding protein for improved nucleic acid amplification technique-based detection of bacterial species in human samples. J Clin Microbiol. 2009, 47: 1050-1057. 10.1128/JCM.02242-08.PubMedCentralPubMed
75.
Lehmann LE, Alvarez J, Hunfeld KP, Goglio A, Kost GJ, Louie RF, Raglio A, Regueiro BJ, Wissing H, Stüber F: Potential clinical utility of polymerase chain reaction in microbiological testing for sepsis. Crit Care Med. 2009, 37: 3085-3090. 10.1097/CCM.0b013e3181b033d7.PubMed
76.
Kaleta EJ, Clark AE, Johnson DR, Gamage DC, Wysocki VH, Cherkaoui A, Schrenzel J, Wolk DM: Use of PCR coupled with electrospray ionization mass spectrometry for rapid identification of bacterial and yeast bloodstream pathogens from blood culture bottles. J Clin Microbiol. 2011, 49: 345-353. 10.1128/JCM.00936-10.PubMedCentralPubMed
77.
Ecker DJ, Sampath R, Massire C, Blyn LB, Hall TA, Eshoo MW, Hofstadler SA: Ibis T5000: a universal biosensor approach for microbiology. Nat Rev Microbiol. 2008, 6: 553-558. 10.1038/nrmicro1918.PubMed
78.
Hall TA, Sampath R, Blyn LB, Ranken R, Ivy C, Melton R, Matthews H, White N, Li F, Harpin V, Ecker DJ, McDougal LK, Limbago B, Ross T, Wolk DM, Wysocki V, Carroll KC: Rapid molecular genotyping and clonal complex assignment of Staphylococcus aureus isolates by PCR coupled to electrospray ionization mass spectrometry. J Clin Microbiol. 2009, 47: 1733-1741. 10.1128/JCM.02175-08.PubMedCentralPubMed
79.
Baldwin CD, Howe GB, Sampath R, Blyn LB, Matthews H, Harpin V, Hall TA, Drader JJ, Hofstadler SA, Eshoo MW, Rudnick K, Studarus K, Moore D, Abbott S, Janda JM, Whitehouse CA: Usefulness of multilocus polymerase chain reaction followed by electrospray ionization mass spectrometry to identify a diverse panel of bacterial isolates. Diagn Microbiol Infect Dis. 2009, 63: 403-408. 10.1016/j.diagmicrobio.2008.12.012.PubMed
80.
Ecker DJ, Sampath R, Li H, Massire C, Matthews HE, Toleno D, Hall TA, Blyn LB, Eshoo MW, Ranken R, Hofstadler SA, Tang YW: New technology for rapid molecular diagnosis of bloodstream infections. Expert Rev Mol Diagn. 2010, 10: 399-415. 10.1586/erm.10.24.PubMed
81.
Sampath R, Hall TA, Massire C, Li F, Blyn LB, Eshoo MW, Hofstadler SA, Ecker DJ: Rapid identification of emerging infectious agents using PCR and electrospray ionization mass spectrometry. Ann N Y Acad Sci. 2007, 1102: 109-120. 10.1196/annals.1408.008.PubMed
82.
Eshoo MW, Crowder CD, Li H, Matthews HE, Meng S, Sefers SE, Sampath R, Stratton CW, Blyn LB, Ecker DJ, Tang YW: Detection and identification of Ehrlichia species in blood using PCR and electrospray ionization mass spectrometry. J Clin Microbiol. 2010, 48: 472-478. 10.1128/JCM.01669-09.PubMedCentralPubMed
83.
Kaleta EJ, Clark AE, Cherkaoui A, Wysocki VH, Ingram EL, Schrenzel J, Wolk DM: Comparative analysis of PCR-electrospray ionization/mass spectrometry(MS) and MALDI-TOF/MS for the identification of bacteria and yeast from positive blood culture bottles. Clin Chem. 2011, 57: 1057-1067. 10.1373/clinchem.2011.161968.PubMed
84.
85.
Bizzini A, Greub G: Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, a revolution in clinical microbial identification. Clin Microbiol Infect. 2010, 16: 1614-1619. 10.1111/j.1469-0691.2010.03311.x.PubMed
86.
Tomas I, Alvarez M, Limeres J, Potel C, Medina J, Diz P: Prevalence, duration and aetiology of bacteraemia following dental extractions. Oral Dis. 2007, 13: 56-62. 10.1111/j.1601-0825.2006.01247.x.PubMed
87.
Dark PM, Dean P, Warhurst G: Bench-to-bedside review: the promise of rapid infection diagnosis during sepsis using polymerase chain reaction-based pathogen detection. Crit Care. 2009, 13: 217-10.1186/cc7886.PubMedCentralPubMed
88.
Dierkes C, Ehrenstein B, Siebig S, Linde HJ, Reischl U, Salzberger B: Clinical impact of a commercially available multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis. BMC Infect Dis. 2009, 9: 126-10.1186/1471-2334-9-126.PubMedCentralPubMed
89.
Lehmann LE, Herpichboehm B, Kost GJ, Kollef MH, Stüber F: Cost and mortality prediction using polymerase chain reaction pathogen detection in sepsis: evidence from three observational trials. Crit Care. 2010, 14: R186-10.1186/cc9294.PubMedCentralPubMed
90.
Maaroufi Y, Ahariz N, Husson M, Crokaert F: Comparison of different methods of isolation of DNA of commonly encountered Candida species and its quantitation by using a real-time PCR-based assay. J Clin Microbiol. 2004, 42: 3159-3163. 10.1128/JCM.42.7.3159-3163.2004.PubMedCentralPubMed
91.
Dineva MA, MahiLum-Tapay L, Lee H: Sample preparation: a challenge in the development of point-of-care nucleic acid-based assays for resource limited settings. Analyst. 2007, 132: 1193-1199. 10.1039/b705672a.PubMed
92.
Klouche M, Schröder U: Rapid methods for diagnosis of bloodstream infections. Clin Chem Lab Med. 2008, 46: 888-908.PubMed
93.
Peters RP, Mohammadi T, Vandenbroucke-Grauls CM, Danner SA, van Agtmael MA, Savelkoul PH: Detection of bacterial DNA in blood samples from febrile patients: underestimated infection or emerging contamination?. FEMS Immunol Med Microbiol. 2004, 42: 249-253. 10.1016/j.femsim.2004.05.009.PubMed
94.
Kline KA, Fälker S, Dahlberg S, Normark S, Henriques-Normark B: Bacterial adhesins in host-microbe interactions. Cell Host Microbe. 2009, 5: 580-592.PubMed
95.
Rubtsova MY, Ulyashova MM, Bachmann TT, Schmid RD, Egorov AM: Multiparametric determination of genes and their point mutations for identification of beta-lactamases. Biochemistry. 2010, 75: 1628-1649.PubMed
96.
Rello J, Lisboa T, Lujan M, Gallego M, Kee C, Kay I, Lopez D, Waterer GW, DNA-Neumococo Study Group: Severity of pneumococcal pneumonia associated with genomic bacterial load. Chest. 2009, 136: 832-840. 10.1378/chest.09-0258.PubMed
97.
Harbarth S, Nobre V, Pittet D: Does antibiotic selection impact patient outcome?. Clin Infect Dis. 2007, 44: 87-93. 10.1086/510075.PubMed
98.
Horisberger T, Harbarth S, Nadal D, Baenziger O, Fischer JE: G-CSF and IL-8 for early diagnosis of sepsis in neonates and critically ill children - safety and cost effectiveness of a new laboratory prediction model. Study protocol of a randomized controlled trial. Crit Care. 2004, 8: R443-450. 10.1186/cc2971.PubMedCentralPubMed
99.
Cohen D, Billingsley M: Europeans are left to their own devices. BMJ. 2011, 342: d2748-10.1136/bmj.d2748.PubMed
100.
Wilmshurst P: The regulation of medical devices. BMJ. 2011, 342: d2822-10.1136/bmj.d2822.PubMed
101.
Fischer J, Harbarth S, Agthe A, Benn A, Ringer S, Goldmann D, Fanconi S: Quantifying uncertainty: physicians' estimates of infection in critically ill neonates and children. Clin Infect Dis. 2004, 38: 1383-1390. 10.1086/420741.PubMed
102.
Forrest GN, Mehta S, Weekes E, Lincalis DP, Johnson JK, Venezia RA: Impact of rapid in situ hybridization testing on coagulase-negative staphylococci positive blood cultures. J Antimicrob Chemother. 2006, 58: 154-158. 10.1093/jac/dkl146.PubMed
103.
Westh H, Lisby G, Breysse F, Böddinghaus B, Chomarat M, Gant V, Goglio A, Raglio A, Schuster H, Stuber F, Wissing H, Hoeft A: Multiplex real-time PCR and blood culture for identification of bloodstream pathogens in patients with suspected sepsis. Clin Microbiol Infect. 2009, 15: 544-551. 10.1111/j.1469-0691.2009.02736.x.PubMed
104.
Lindholm L, Sarkkinen H: Direct identification of gram-positive cocci from routine blood cultures by using AccuProbe tests. J Clin Microbiol. 2004, 42: 5609-5613. 10.1128/JCM.42.12.5609-5613.2004.PubMedCentralPubMed
105.
Arbique JC, Poyart C, Trieu-Cuot P, Quesne G, Carvalho Mda G, Steigerwalt AG, Morey RE, Jackson D, Davidson RJ, Facklam RR: Accuracy of phenotypic and genotypic testing for identification of Streptococcus pneumoniae and description of Streptococcus pseudopneumoniae sp. nov. J Clin Microbiol. 2004, 42: 4686-4696. 10.1128/JCM.42.10.4686-4696.2004.PubMedCentralPubMed
106.
Metadata
Title
Bench-to-bedside review: Rapid molecular diagnostics for bloodstream infection - a new frontier?
Authors
Arash Afshari
Jacques Schrenzel
Margareta Ieven
Stephan Harbarth
Publication date
01-06-2012
Publisher
BioMed Central
Published in
Critical Care / Issue 3/2012
Electronic ISSN: 1364-8535
DOI
https://doi.org/10.1186/cc11202

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