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Obesity Surgery
Published in: Obesity Surgery 1/2011

01-01-2011 | Clinical Research

Bariatric Surgery Reverses Natural Killer (NK) Cell Activity and NK-Related Cytokine Synthesis Impairment Induced by Morbid Obesity

Authors: Cristiane Martins Moulin, Ivo Marguti, Jean Pierre Schatzmann Peron, Alfredo Halpern, Luiz Vicente Rizzo

Published in: Obesity Surgery | Issue 1/2011

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Abstract

Background

Obesity is related to a higher rate of infections and some types of cancer. Here we analyzed the impact of obesity and weight loss induced by Roux-en-Y gastric bypass (RYGB) on immunological parameters, i.e., cytokine productions and natural killer cell function.

Methods

We analyzed 28 morbidly obese patients before and 6 months after RYGB. Biochemical parameters were analyzed in plasma. The percent of natural killer (NK) cells, their cytotoxicity, and the production of cytokines by peripheral blood mononuclear cells were analyzed. The percent of NK cells was determined by flow cytometry and cytokine production determined by enzyme-linked immunosorbent assay. NK cytotoxicity was determined by the lactate dehydrogenase release assay.

Results

The weight loss 6 months following surgery was 35.3 ± 4.5 kg. RYGB also improves biochemical parameters. No significant difference was found in the percent of NK cells after surgery. We found an increase in the production of interferon-γ, interleukin (IL)-12 and IL-18, but not in IL-2, 6 months after RYGB. Cytotoxic activity of NK cells was significantly enhanced 6 months after RYGB [17.1 ± 14.7% before RYGB vs 51.8 ± 11.3% at 6 months after, at 40:1 effector to target cell ratio; p < 0.001]. We observed significant post-surgical improvement in the cytotoxic activity curve in 22 out of 28 patients (78.6%), irrespective of the target to effector cell ratio.

Conclusions

The weight loss induced by RYGB modifies the production of cytokines related with NK cell function and improves its activity.
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Metadata
Title
Bariatric Surgery Reverses Natural Killer (NK) Cell Activity and NK-Related Cytokine Synthesis Impairment Induced by Morbid Obesity
Authors
Cristiane Martins Moulin
Ivo Marguti
Jean Pierre Schatzmann Peron
Alfredo Halpern
Luiz Vicente Rizzo
Publication date
01-01-2011
Publisher
Springer-Verlag
Published in
Obesity Surgery / Issue 1/2011
Print ISSN: 0960-8923
Electronic ISSN: 1708-0428
DOI
https://doi.org/10.1007/s11695-010-0250-8

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