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BMC Oral Health
Published in: BMC Oral Health 1/2018

Open Access 01-12-2018 | Research article

Bactericidal effect of a diode laser on Enterococcus faecalis in human primary teeth—an in vitro study

Authors: Shanshan Dai, Gang Xiao, Ning Dong, Fei Liu, Shuyang He, Qingyu Guo

Published in: BMC Oral Health | Issue 1/2018

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Abstract

Background

In recent years, the diode laser (810 nm) has been used for root canal disinfection, which plays an important role in endodontic therapy. This study was undertaken to evaluate the disinfecting ability of a diode laser in experimentally infected root canals of primary teeth.

Methods

Human retained mandibular primary anterior teeth without apical foramen resorption were selected and contaminated with Enterococcus faecalis for 21 days. The specimens were randomly divided into four groups: the negative group (no treatment), positive group (5.25% NaOCl), diode laser group (diode laser), and diode-NaOCl group (diode laser combined with NaOCl). The disinfecting abilities of the treatments were measured by the numbers of bacteria, scanning electron microscopy and confocal laser microscopy (live-dead staining).

Results

Eighty teeth were selected. After irradiation and irrigation, the elimination of bacteria and the smear layer in the laser groups and positive group were significantly superior, compared with the negative group (p < 0.01). In the diode-NaOCl group, bacterial reduction reached nearly 100% on the surfaces of root canals; live bacteria were rarely observed, even in deeper dentinal tubules.

Conclusion

Use of a diode laser, especially in combination with NaOCl, was effective for disinfecting infected root canals of primary teeth.
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Metadata
Title
Bactericidal effect of a diode laser on Enterococcus faecalis in human primary teeth—an in vitro study
Authors
Shanshan Dai
Gang Xiao
Ning Dong
Fei Liu
Shuyang He
Qingyu Guo
Publication date
01-12-2018
Publisher
BioMed Central
Published in
BMC Oral Health / Issue 1/2018
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/s12903-018-0611-6

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