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BMC Oral Health
Published in: BMC Oral Health 1/2023

Open Access 01-12-2023 | Research

Bacterial diversity in primary infected root canals of a Chinese cohort: analysis of 16 S rDNA sequencing

Authors: Ziqiu Hu, Yonggang Xiang, Yanhong Wei, Xinsheng Gu, Weidong Leng, Lingyun Xia

Published in: BMC Oral Health | Issue 1/2023

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Abstract

Purpose

To characterize the bacterial community in the primarily infected root canals.

Methods

A total of 13 samples were collected from the primarily infected root canals. 16 S rDNA sequencing was performed to define bacterial community. Taxonomic annotation, bacterial hierarchical structures, community richness and diversity, and inter-subject variability of the bacterial community in the root canal samples were analyzed. Gender, age, and duration of the toothache-specific bacterial community associated with the patient groups were analyzed.

Results

A total of 359 Species were annotated and identified in the whole study cohort. The Alpha diversity analysis showed that the species diversity and detection rate of the 13 samples were high, which reflected the authenticity of sequencing results. The Beta diversity analysis was used to compare the degree of difference between different root canal samples. The 13 samples were divided into two groups according to the results, group A was samples I1-I12, and group B was samples I13. The bacterial species of group A samples were analyzed with the clinical characteristics of patients, and it was found that gender, and duration specific differences in bacterial species, and there was no significant difference in species types among different ages of patients.

Conclusion

There were a wide diversity and inter-subject variability in the bacterial community in the primary infected root canals. While Porphyromonas gingivalis was the most abundant species, Fusobacterium nucleatum was the most variable species in the bacterial community of the root canal. The bacterial community at different taxonomic levels varied from sample to sample, despite consistent disease diagnoses. There was gender, duration-specific differences in the bacterial species in the primary infected root canals.
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Metadata
Title
Bacterial diversity in primary infected root canals of a Chinese cohort: analysis of 16 S rDNA sequencing
Authors
Ziqiu Hu
Yonggang Xiang
Yanhong Wei
Xinsheng Gu
Weidong Leng
Lingyun Xia
Publication date
01-12-2023
Publisher
BioMed Central
Published in
BMC Oral Health / Issue 1/2023
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/s12903-023-03618-3

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