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International Orthopaedics
Published in: International Orthopaedics 8/2012

01-08-2012 | Original Paper

Autologous transplantation of culture-born myofibroblasts into intact and injured rabbit ligaments

Authors: Thomas Laumonier, Marlene Michel, Giulio Gabbiani, Pierre Hoffmeyer, Marie-Luce Bochaton-Piallat, Jacques Menetrey

Published in: International Orthopaedics | Issue 8/2012

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Abstract

Purpose

The myofibroblast, a contractile fibroblastic cell expressing α-smooth muscle actin (α-SMA), has been reported to play a role in ligament healing. The aim of this study was to evaluate the feasibility of transplanting culture-derived myofibroblasts in injured rabbit medial collateral ligaments (MCL) and in intact anterior cruciate ligaments (ACL).

Methods

Fibroblasts isolated from the iliotibial band were cultured in the presence of transforming growth factor beta-1 (TGF-β1) for five days and analysed for α-SMA expression. In a concentration of TGF-β1 ≥ 10 ng/ml, the differentiation rate into myofibroblast was 90%. After labelling with PKH26, α-SMA -positive cells were transplanted in intact ACL and in injured MCL of ten rabbits.

Results

Survival of PKH-26+ cells was seen in all intact and damaged ligaments one day after injection. The density of PKH-26+ cells had decreased at seven days postinjection in both ligaments. Double-positive PKH-26+/α-SMA+ cells were only observed in injured MCL at seven days postinjection. Moreover, we found that genetically modified fibroblasts differentiate into myofibroblasts and can be transplanted into ligaments.

Conclusions

Our data demonstrate that culture-born myofibroblasts survive and maintain α-SMA expression up to one week after transplantation. This study provides the first insight into the feasibility of transplanted mechanically active cells for ligament reconstruction.
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Metadata
Title
Autologous transplantation of culture-born myofibroblasts into intact and injured rabbit ligaments
Authors
Thomas Laumonier
Marlene Michel
Giulio Gabbiani
Pierre Hoffmeyer
Marie-Luce Bochaton-Piallat
Jacques Menetrey
Publication date
01-08-2012
Publisher
Springer-Verlag
Published in
International Orthopaedics / Issue 8/2012
Print ISSN: 0341-2695
Electronic ISSN: 1432-5195
DOI
https://doi.org/10.1007/s00264-012-1519-4

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