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Journal of Orthopaedic Surgery and Research
Published in: Journal of Orthopaedic Surgery and Research 1/2019

Open Access 01-12-2019 | Research article

Autologous stem cell-derived chondrocyte implantation with bio-targeted microspheres for the treatment of osteochondral defects

Authors: Murat Bozkurt, Mehmet Doğan Aşık, Safa Gürsoy, Mustafa Türk, Siyami Karahan, Berrak Gümüşkaya, Mustafa Akkaya, Mehmet Emin Şimşek, Nurdan Cay, Metin Doğan

Published in: Journal of Orthopaedic Surgery and Research | Issue 1/2019

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Abstract

Background

Chondral injury is a common problem around the world. Currently, there are several treatment strategies for these types of injuries. The possible complications and problems associated with conventional techniques lead us to investigate a minimally invasive and biotechnological alternative treatment. Combining tissue-engineering and microencapsulation technologies provide new direction for the development of biotechnological solutions. The aim of this study is to develop a minimal invasive tissue-engineering approach, using bio-targeted microspheres including autologous cells, for the treatment of the cartilage lesions.

Method

In this study, a total of 28 sheeps of Akkaraman breed were randomly assigned to one of the following groups: control (group 1), microfracture (group 2), scaffold (group 3), and microsphere (group 4). Microspheres and scaffold group animals underwent adipose tissue collection prior to the treatment surgery. Mesenchymal cells collected from adipose tissue were differentiated into chondrocytes and encapsulated with scaffolds and microspheres. Osteochondral damage was conducted in the right knee joint of the sheep to create an animal model and all animals treated according to study groups.

Results

Both macroscopic and radiologic examination showed that groups 3 and 4 have resulted better compared to the control and microfracture groups. Moreover, histologic assessments indicate hyaline-like cartilage formations in groups 3 and 4.

Conclusion

In conclusion, we believe that the bio-targeted microspheres can be a more effective, easier, and safer approach for cartilage tissue engineering compared to previous alternatives.
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Metadata
Title
Autologous stem cell-derived chondrocyte implantation with bio-targeted microspheres for the treatment of osteochondral defects
Authors
Murat Bozkurt
Mehmet Doğan Aşık
Safa Gürsoy
Mustafa Türk
Siyami Karahan
Berrak Gümüşkaya
Mustafa Akkaya
Mehmet Emin Şimşek
Nurdan Cay
Metin Doğan
Publication date
01-12-2019
Publisher
BioMed Central
Published in
Journal of Orthopaedic Surgery and Research / Issue 1/2019
Electronic ISSN: 1749-799X
DOI
https://doi.org/10.1186/s13018-019-1434-0

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