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World Journal of Surgery
Published in: World Journal of Surgery 6/2007

01-06-2007

Attenuation of Kupffer Cell Function in Acute on Chronic Liver Injury Enhanced Engraftment of Transplanted Hepatocytes

Authors: Ray-Hwang Yuan, Hui-Ling Chen, Huey-Ling Chen, Ming-Kung Hsu, Po-Huang Lee, Mei-Hwei Chang

Published in: World Journal of Surgery | Issue 6/2007

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Abstract

Background

The present study was designed to elucidate the relationship of engraftment efficiency of transplanted cells and Kupffer cell function in mice with acute on chronic liver injury and acute liver injury.

Methods

The recipient dipeptidyl peptidase IV knockout (DPPIV–/–) mice were divided into two groups: (1) the acute on chronic liver injury group (CCl4/APAP group) that received CCl4 (1 ml/kg) twice a week for 4 weeks following one dose of acetaminophen (APAP), 600 mg/kg; (2) the acute liver injury group (APAP-only group) that received a single dose of APAP at 600 mg/kg. DPPIV+/+ hepatocytes were transplanted 24 h after APAP intoxication. Engraftment efficiency was evaluated at day 7 and day 14 after transplantation. The tumor necrosis factor-α (TNF-α) mRNA expression level of Kupffer cells immediately before cell transplantation was compared between the two groups before and after lipopolysaccharide (LPS, 100 ng/ml) stimulation.

Results

The number of transplanted cells and clusters in each 100× microscopic field were higher in the CCl4/APAP group at both day 7 (21.5 ± 6.3 versus 8.3 ± 4.0, p < 0.001; 14.9 ± 4.6 versus 6.6 ± 3.4, p < 0.001, respectively) and day 14 (17.3 ± 4.4 versus 10.2 ± 3.3, p = 0.001; 12.6 ± 3.2 versus 7.9 ± 1.6, p = 0.004, respectively). After LPS stimulation, the expression level of TNF-α was lower (175.7 ± 54.6 versus 465.6 ± 64.2, p = 0.002), and the increment of TNF-α expression was also less significant in the CCl4/APAP group (1.5-fold versus 6.5-fold, p = 0.014).

Conclusions

Chronic liver injury desensitized Kupffer cells and reduced TNF-α expression, two results that correlated with the increased engraftment of transplanted cells.
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Metadata
Title
Attenuation of Kupffer Cell Function in Acute on Chronic Liver Injury Enhanced Engraftment of Transplanted Hepatocytes
Authors
Ray-Hwang Yuan
Hui-Ling Chen
Huey-Ling Chen
Ming-Kung Hsu
Po-Huang Lee
Mei-Hwei Chang
Publication date
01-06-2007
Publisher
Springer-Verlag
Published in
World Journal of Surgery / Issue 6/2007
Print ISSN: 0364-2313
Electronic ISSN: 1432-2323
DOI
https://doi.org/10.1007/s00268-007-9044-0

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