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BMC Complementary Medicine and Therapies

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Open Access 01-12-2015 | Research article

Attenuated RANKL-induced cytotoxicity by Portulaca oleracea ethanol extract enhances RANKL-mediated osteoclastogenesis

Authors: Munkhsoyol Erkhembaatar, Eun-Joo Choi, Hak-Yong Lee, Choong Hun Lee, Young-Rae Lee, Min Seuk Kim

Published in: BMC Complementary Medicine and Therapies | Issue 1/2015

Abstract

Background

Portulaca oleracea (PO) has been widely used as traditional medicine because of its pharmacological activities. However, the effects of PO on osteoclasts that modulate bone homeostasis are still elusive.

Methods

In this study, we examined the effects of PO ethanol extract (POEE) on receptor activator of nuclear factor-κB ligand (RANKL)-mediated Ca²⁺ mobilization, nuclear factor of activated T-cell c1 (NFATc1) amplification, tartrate-resistant acid phosphatase-positive (TRAP+) multinucleated cell (MNC) formation, and cytotoxicity.

Results

Our results demonstrated that POEE suppressed RANKL-induced Ca²⁺ oscillations by inhibition of Ca²⁺ release from internal Ca²⁺ stores, resulting in reduction of NFATc1 amplification. Notably, POEE attenuated RANKL-mediated cytotoxicity and cleavage of polyadenosine 5′-diphosphate-ribose polymerase (PARP), resulted in enhanced formation of TRAP+ MNCs.

Conclusions

These results present in vitro effects of POEE on RANKL-mediated osteoclastogenesis and suggest the possible use of PO in treating bone disorders, such as osteopetrosis.

Xu Z, Shan Y. Anti-fatigue effects of polysaccharides extracted from Portulaca oleracea L. in mice. Indian J Biochem Biophysics. 2014;51(4):321–5.

Wang CQ, Yang GQ. Betacyanins from Portulaca oleracea L. ameliorate cognition deficits and attenuate oxidative damage induced by D-galactose in the brains of senescent mice. Phytomed. 2010;17(7):527–32.CrossRef

Lee S, Kim KH, Park C, Lee JS, Kim YH. Portulaca oleracea extracts protect human keratinocytes and fibroblasts from UV-induced apoptosis. Exp Dermatol. 2014;23 Suppl 1:13–7.CrossRefPubMed

Farshori NN, Al-Sheddi ES, Al-Oqail MM, Musarrat J, Al-Khedhairy AA, Siddiqui MA. Cytotoxicity assessments of Portulaca oleracea and Petroselinum sativum seed extracts on human hepatocellular carcinoma cells (HepG2). Asian Pacific J Cancer Prev. 2014;15(16):6633–8.CrossRef

Takayanagi H, Kim S, Koga T, Nishina H, Isshiki M, Yoshida H, et al. Induction and activation of the transcription factor NFATc1 (NFAT2) integrate RANKL signaling in terminal differentiation of osteoclasts. Dev Cell. 2002;3(6):889–901.CrossRefPubMed

Koga T, Inui M, Inoue K, Kim S, Suematsu A, Kobayashi E, et al. Costimulatory signals mediated by the ITAM motif cooperate with RANKL for bone homeostasis. Nature. 2004;428(6984):758–63.CrossRefPubMed

Kim MS, Yang YM, Son A, Tian YS, Lee SI, Kang SW, et al. RANKL-mediated reactive oxygen species pathway that induces long lasting Ca2+ oscillations essential for osteoclastogenesis. J Biol Chem. 2010;285(10):6913–21.CrossRefPubMedPubMedCentral

Bharti AC, Takada Y, Shishodia S, Aggarwal BB. Evidence that receptor activator of nuclear factor (NF)-kappaB ligand can suppress cell proliferation and induce apoptosis through activation of a NF-kappaB-independent and TRAF6-dependent mechanism. J Biol Chem. 2004;279(7):6065–76.CrossRefPubMed

Kim MS, Zeng W, Yuan JP, Shin DM, Worley PF, Muallem S. Native Store-operated Ca2+ Influx Requires the Channel Function of Orai1 and TRPC1. J Biol Chem. 2009;284(15):9733–41.CrossRefPubMedPubMedCentral

10.

Hwang JK, Erkhembaatar M, Gu DR, Lee SH, Lee CH, Shin DM, et al. Glechoma hederacea Suppresses RANKL-mediated Osteoclastogenesis. J Dent Res. 2014;93(7):685–90.CrossRefPubMedPubMedCentral

11.

Park B, Yang YM, Choi BJ, Kim MS, Shin DM. Activation of G Proteins by Aluminum Fluoride Enhances RANKL-Mediated Osteoclastogenesis. Korean J Physiol Pharma. 2013;17(5):427–33.CrossRef

12.

Yang YM, Jung HH, Lee SJ, Choi HJ, Kim MS, Shin DM. TRPM7 Is Essential for RANKL-Induced Osteoclastogenesis. Korean J Physiol Pharmacol. 2013;17(1):65–71.CrossRefPubMedPubMedCentral

13.

Yang YM, Kim MS, Son A, Hong JH, Kim KH, Seo JT, et al. Alteration of RANKL-induced osteoclastogenesis in primary cultured osteoclasts from SERCA2+/- mice. J Bone Min Res. 2009;24(10):1763–9.CrossRef

14.

Weidema AF, Dixon SJ, Sims SM. Activation of P2Y but not P2X(4) nucleotide receptors causes elevation of [Ca2+]i in mammalian osteoclasts. Am J Physiol Cell Physiol. 2001;280(6):C1531–1539.PubMed

15.

Korcok J, Raimundo LN, Ke HZ, Sims SM, Dixon SJ. Extracellular nucleotides act through P2X7 receptors to activate NF-kappaB in osteoclasts. J Bone Min Res. 2004;19(4):642–51.CrossRef

16.

Fuller K, Wong B, Fox S, Choi Y, Chambers TJ. TRANCE is necessary and sufficient for osteoblast-mediated activation of bone resorption in osteoclasts. J Exp Med. 1998;188(5):997–1001.CrossRefPubMedPubMedCentral

17.

Nunez G, Benedict MA, Hu Y, Inohara N. Caspases: the proteases of the apoptotic pathway. Oncogene. 1998;17(25):3237–45.CrossRefPubMed

18.

Sugatani T, Alvarez U, Hruska KA. PTEN regulates RANKL- and osteopontin-stimulated signal transduction during osteoclast differentiation and cell motility. J biol Chem. 2003;278(7):5001–8.CrossRefPubMed

19.

Miyamoto T. Regulators of osteoclast differentiation and cell-cell fusion. Keio J Med. 2011;60(4):101–5.CrossRefPubMed

Title: Attenuated RANKL-induced cytotoxicity by Portulaca oleracea ethanol extract enhances RANKL-mediated osteoclastogenesis
Authors: Munkhsoyol Erkhembaatar
Eun-Joo Choi
Hak-Yong Lee
Choong Hun Lee
Young-Rae Lee
Min Seuk Kim
Publication date: 01-12-2015
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2015
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-015-0770-9

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Attenuated RANKL-induced cytotoxicity by Portulaca oleracea ethanol extract enhances RANKL-mediated osteoclastogenesis

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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