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International Journal of Colorectal Disease

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01-09-2018 | Original Article

Association of ulcerative colitis with solute-linked carrier family 26 member A3 gene polymorphisms and its expression in colonic tissues in Chinese patients

Authors: Xiao-xiao Shao, Dao-po Lin, Liang Sun, Chao-qun Wu, Wei Yang, Yi Jiang

Published in: International Journal of Colorectal Disease | Issue 9/2018

Abstract

Purpose

Abnormalities of the solute-linked carrier family 26 member A3 (SLC26A3) are implicated in the pathogenesis of several diseases including ulcerative colitis (UC). The short communication aimed at investigating the associations of UC with SLC26A3 (rs17154444, rs7810937, rs7785539, rs2108225 and rs6951457) polymorphisms and its expression in colonic tissues.

Methods

The techniques of SNaPshot method, quantitative real-time PCR and immunohistochemical analysis were conducted.

Results and conclusion

We found that the rs2108225 variation in SLC26A3 might increase the risk of UC and affect its expression at both the mRNA and protein levels in colonic tissues of patients with UC. Moreover, the rs17154444 variation might influence the severity of UC.

Xiao F, Juric M, Li J et al (2012) Loss of downregulated in adenoma (DRA) impairs mucosal HCO3(-) secretion in murine ileocolonic inflammation. Inflamm Bowel Dis 18(1):101–111. https://doi.org/10.1002/ibd.21744 CrossRefPubMed

Wojtal KA, Eloranta JJ, Hruz P (2009) Changes in mRNA expression levels of solute carrier transporters in inflammatory bowel disease patients. Drug Metab Dispos 37(9):1871–1877. https://doi.org/10.1124/dmd.109.027367 CrossRefPubMed

Ghishan FK, Kiela PR (2014) Epithelial transport in inflammatory bowel diseases. Inflamm Bowel Dis 20(6):1099–1109. https://doi.org/10.1097/MIB.0000000000000029. PubMedPubMedCentralCrossRef

Ishimori S, Kaito H, Matsunoshita N et al (2013) SLC26A3 gene analysis in patients with Bartter and Gitelman syndromes and the clinical characteristics of patients with unidentified mutations. Kobe J Med Sci 59(2):E36–E43. https://doi.org/10.1016/j.mam.2012.07.009 PubMedCrossRef

Hu D-y, Shao X-x, Xu C-l et al (2014) Associations of FUT2 and FUT3 gene polymorphisms with Crohn’s disease in Chinese patients. J Gastroenterol Hepatol 29(10):1778–1785. https://doi.org/10.1111/jgh.12599 CrossRefPubMed

Borenshtein D, Schlieper KA, Rickman BH et al (2009) Decreased expression of colonic Slc26a3 and carbonic anhydrase iv as a cause of fatal infectious diarrhea in mice. Infect Immun 77(9):3639–3650. https://doi.org/10.1128/IAI.00225-09 CrossRefPubMedPubMedCentral

Asano K, Matsushita T, Umeno J et al (2009) A genome-wide association study identifies three new susceptibility loci for ulcerative colitis in the Japanese population. Nat Genet 41(12):1325–1329. https://doi.org/10.1038/ng.482 CrossRefPubMed

Asano K, Esaki M, Umeno J et al (2015) Contribution of susceptibility variants at FCGR2A and 13q12 to the risk of relapse among Japanese patients with ulcerative colitis. J Gastroenterol 50(11):1094–1102. https://doi.org/10.1007/s00535-015-1062-3 CrossRefPubMed

Yang SK, Jung Y, Kim H et al (2011) Association of FCGR2A, JAK2 or HNF4A variants with ulcerative colitis in Koreans. Dig Liver Dis 43(11):856–861. https://doi.org/10.1016/j.dld.2011.07.006 CrossRefPubMed

10.

Liu JZ, van Sommeren S, Huang H et al (2015) Association analyses identify 38 susceptibility loci for inflammatory bowel disease and highlight shared genetic risk across populations. Nat Genet 47(9):979–986. https://doi.org/10.1038/ng.3359 CrossRefPubMedPubMedCentral

11.

Cleynen I, Boucher G, Jostins L et al (2016) Inherited determinants of Crohn’s disease and ulcerativecolitis phenotypes: a genetic association study. Lancet 387:156–167. https://doi.org/10.1016/S0140-6736(15)00465-1 CrossRefPubMedPubMedCentral

12.

Moyer RA, Wang D, Papp AC et al (2011) Intronic polymorphisms affecting alternative splicing of human dopamine D2 receptor are associated with cocaine abuse. Neuropsychopharmacology 36(4):753–762. https://doi.org/10.1038/npp.2010.208 CrossRefPubMed

Title: Association of ulcerative colitis with solute-linked carrier family 26 member A3 gene polymorphisms and its expression in colonic tissues in Chinese patients
Authors: Xiao-xiao Shao
Dao-po Lin
Liang Sun
Chao-qun Wu
Wei Yang
Yi Jiang
Publication date: 01-09-2018
Publisher: Springer Berlin Heidelberg
Published in: International Journal of Colorectal Disease / Issue 9/2018
Print ISSN: 0179-1958
Electronic ISSN: 1432-1262
DOI: https://doi.org/10.1007/s00384-018-3097-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Association of ulcerative colitis with solute-linked carrier family 26 member A3 gene polymorphisms and its expression in colonic tissues in Chinese patients

Abstract

Purpose

Methods

Results and conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results and conclusion

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