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Journal of Assisted Reproduction and Genetics

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01-05-2019 | Genetics

Association of genetic polymorphism of vascular endothelial growth factor in the etiology of recurrent pregnancy loss: a triad study

Authors: M. Vidyadhari, M. Sujatha, P. Krupa, Pratibha Nallari, A. Venkateshwari

Published in: Journal of Assisted Reproduction and Genetics | Issue 5/2019

Abstract

Purpose

The study estimates the association of VEGF gene polymorphism (-1154 G/A, -2549 I/D, -2578 C/A, and +936 C/T) in recurrent pregnancy loss from South Indian population.

Methods

A total of 100 couples with the history of recurrent pregnancy loss and 100 couples with medically terminated pregnancies were considered. Fetal tissues with < 20 weeks of gestation including peripheral blood from case and control couples were collected. VEGF gene polymorphisms were determined by allele-specific polymerase chain reaction. Genotypic distribution and allele frequencies were evaluated by odds ratio with 95% confidence intervals. Haplotype analysis was done to determine the association of specific haplotypes with recurrent pregnancy loss.

Results

The VEGF -1154 G/A polymorphism was significantly prevalent in the aborted fetuses and in their mothers whereas -2549 I/D polymorphism was significantly higher in the aborted fetuses while the + 936 C/T polymorphism showed prevalence in the case mothers revealing their statistically significant association to recurrent pregnancy loss. A₁₁₅₄D₂₅₄₉A₂₅₇₈T₉₃₆ haplotype showed an increased risk in case fetuses and mothers whereas A₁₁₅₄D₂₅₄₉C₂₅₇₈C₉₃₆, in case mothers and fathers while haplotype G₁₁₅₄I₂₅₄₉A₂₅₇₈C₉₃₆ found a protective association in the case fetuses compared to controls.

Conclusion

This is the first report of family-based triad study revealing a significant association of VEGF gene polymorphism in the etiology of recurrent pregnancy loss.

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Title: Association of genetic polymorphism of vascular endothelial growth factor in the etiology of recurrent pregnancy loss: a triad study
Authors: M. Vidyadhari
M. Sujatha
P. Krupa
Pratibha Nallari
A. Venkateshwari
Publication date: 01-05-2019
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 5/2019
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-019-01431-y

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Association of genetic polymorphism of vascular endothelial growth factor in the etiology of recurrent pregnancy loss: a triad study

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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