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Cancer Causes & Control
Published in: Cancer Causes & Control 10/2011

01-10-2011 | Original paper

Association of circulating VacA-neutralizing antibodies with gastric cancer and duodenal ulcer

Authors: Guadalupe Ayala, Lourdes Flores-Luna, Dulce Hernández-Amaro, Guillermo Mendoza-Hernández, Lilia Chihu-Amparán, Fernando Bernal-Sahagún, Margarita Camorlinga, Eduardo Lazcano-Ponce, Javier Torres

Published in: Cancer Causes & Control | Issue 10/2011

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Abstract

Objective

To study the association between anti-VacA antibodies and pre-neoplastic lesions (IM), gastric cancer (GC), and duodenal ulcer (DU).

Methods

A case–control study that included 347 patients, 90 with IM, 60 with GC, 52 with DU, and 145 with non-atrophic gastritis was conducted. For the analysis, a polytomous logistic regression models were used. Anti-VacA antibodies were identified in sera from these patients, either by Western blot assay (WB), using antigens produced by H. pylori s1m1 strain, or by neutralization assay challenging HeLa cells with H. pylori VacA s1m1 cytotoxin.

Results

Results of the WB assay showed no association between WB-anti-VacA antibodies and gastroduodenal diseases. In contrast, when antibodies that neutralize VacA cytotoxic activity were studied, a significant association was found with IM (OR 2.7, 95% CI 1.4–5.1) and DU (OR 2.3, 95% CI 1.1–4.9) and an even stronger association with GC (OR 3.9, 95% CI 1.8–8.5). A significant association with histological subtypes of GC (diffuse and intestinal) and of IM (complete and incomplete) was also found. In addition, the association showed a significant dose–response effect in the case of GC, but not of DU or IM. These associations did not change substantially after adjustment for confounding factors.

Main conclusion

This study showed that VacA-neutralizing antibodies are significantly associated with gastroduodenal diseases, especially GC, and that they might be used as risk markers of GC and DU.
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Metadata
Title
Association of circulating VacA-neutralizing antibodies with gastric cancer and duodenal ulcer
Authors
Guadalupe Ayala
Lourdes Flores-Luna
Dulce Hernández-Amaro
Guillermo Mendoza-Hernández
Lilia Chihu-Amparán
Fernando Bernal-Sahagún
Margarita Camorlinga
Eduardo Lazcano-Ponce
Javier Torres
Publication date
01-10-2011
Publisher
Springer Netherlands
Published in
Cancer Causes & Control / Issue 10/2011
Print ISSN: 0957-5243
Electronic ISSN: 1573-7225
DOI
https://doi.org/10.1007/s10552-011-9817-5

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