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Published in: Virchows Archiv 2/2007

01-02-2007 | Original Article

Association of CDK4 and CCND1 mRNA overexpression in laryngeal squamous cell carcinomas occurs without CDK4 amplification

Authors: Alfons Nadal, Pedro Jares, Magda Pinyol, Laura Conde, Coia Romeu, Pedro L. Fernández, Elías Campo, Antonio Cardesa

Published in: Virchows Archiv | Issue 2/2007

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Abstract

CDK4 is involved in the control of G1–S phase transition as a part of the CCND1/CDK4 complexes. CCND1 and CDK4 gene alterations have been implicated in the development of different tumors. CCND1 has been associated with progression in laryngeal carcinomas. CDK4 protein overexpression was described associated to CCND1 overexpression in these tumors. However, the mechanisms implicated were not known. We analyzed CDK4 gene alterations and mRNA expression in a series of carcinomas of the larynx, and the results were compared to CCND1 expression and clinicopathological characteristics of the patients. CDK4 mRNA was overexpressed in 42 out of 60 tumors (70%) associated with CCND1 mRNA overexpression because 15 out of 16 cases with high CCND1 levels showed simultaneous increased levels of CDK4 mRNA (p = 0.023) and 12 (87%) of the tumors overexpressing both genes were in stage 4. No CDK4 gene amplifications, rearrangements, or mutations were detected in any of the tumors, including 24 overexpressed cases. These findings confirm that CDK4 overexpression is a frequent phenomenon in laryngeal carcinomas, which occurs at the transcriptional level but is related neither to gene amplification nor to gene mutation, and suggest that cooperation with CCND1 may be involved in the progression of laryngeal tumors.
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Metadata
Title
Association of CDK4 and CCND1 mRNA overexpression in laryngeal squamous cell carcinomas occurs without CDK4 amplification
Authors
Alfons Nadal
Pedro Jares
Magda Pinyol
Laura Conde
Coia Romeu
Pedro L. Fernández
Elías Campo
Antonio Cardesa
Publication date
01-02-2007
Publisher
Springer-Verlag
Published in
Virchows Archiv / Issue 2/2007
Print ISSN: 0945-6317
Electronic ISSN: 1432-2307
DOI
https://doi.org/10.1007/s00428-006-0314-2

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