Top

Published in:

01-04-2019 | Original Article

Assessment of the anti-anemic effect of aqueous extract of Allium eriophyllum Boiss leaf in phenylhydrazine-treated Wistar male rats

Authors: Mohammad Mahdi Zangeneh, Mohsen Zhaleh, Saman Salmani, Akram Zangeneh, Maryam Almasi, Mohammad Reza Khedri, Khodabakhsh Rashidi

Published in: Comparative Clinical Pathology | Issue 2/2019

Abstract

There are many medicinal plants in Iranian traditional medicine which are used to treat anemia. One of these plants is Allium eriophyllum Boiss. The aim of this study was to investigate the anti-anemic potential of aqueous extract of A. eriophyllum leaf. After collection of the plant, its extract was obtained using Soxhlet extractor. In this study, 60 rats were used. Induction of hemolytic anemia was done by three injections of phenylhydrazine in 50 animals. After 1 day, the rats were divided into six subgroups, including negative healthy control, untreated negative control, and four groups receiving the A. eriophyllum at 20, 40, 80, and 160 mg/kg concentrations. At the end of day 15 of treatment, the animals of all groups were sacrificed, and blood samples were drawn immediately from the animals’ hearts to analyze the biochemical and hematological parameters. The data were analyzed by SPSS-21 software. Different doses of A. eriophyllum significantly (p ≤ 0.05) reduced the raised levels of alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transferase (GGT), urea, creatinine, ferrous, ferritin, and erythropoietin and increased the levels of body weight, white blood cell (WBC), lymphocyte, neutrophils, monocytes, platelet, red blood cell (RBC), hemoglobin (Hb), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) as compared to the untreated group. Seemingly, aqueous extract of A. eriophyllum can be used for the treatment of hemolytic anemia.

Beaven GH, White JC (1954) Oxidation of phenylhydrazines in the presence of oxyhaemoglobin and the origin of Heinz bodies in erythrocytes. Nature 27:389–391CrossRef

Bjelakovic G, Nikolova D, Simonetti RG, Gluud C (2004) Antioxidant supplements for prevention of gastrointestinal cancers: a systematic review and meta-analysis. Lancet 364:1219–1228PubMedCrossRef

Boretti FS, Buehler PW, D'Agnillo F, Kluge K, Glaus T, Butt OI, Jia Y, Goede J, Pereira CP, Maggiorini M, Schoedon G, Alayash AI, Schaer DJ (2009) Sequestration of extracellular hemoglobin within a haptoglobin complex decreases its hypertensive and oxidative effects in dogs and guinea pigs. J Clin Invest 119(8):2271–2280PubMedPubMedCentral

Bratosin D, Mazurier J, Tissier JP, Estaquier J, Huart JJ, Ameisen JC, Aminoff D, Montreuil J (1998) Cellular and molecular mechanisms of senescent erythrocyte phagocytosis by macrophages. A review. Biochimie 80(2):173–195PubMedCrossRef

Cheraghi M, Asadi-Samani M (2016) Hematopoietic medicinal plants based on ethnobotanical documents of Iran: a strategy to develop nature-based drugs effective on anemia. Pharm Lett 8(5):393–399

Delaquis PJ, Mazza G (1995) Antimicrobial properties of isothiocyanates in food preservation. J Food Technol 49(11):73–84

Farzaei MH, Zangeneh MM, Goodarzi N, Zangeneh A (2018) Stereological assessment of nephroprotective effects of Trachyspermum ammi essential oil against carbon tetrachloride-induced nephrotoxicity in mice. Int J Morphol 36(2):750–757CrossRef

Ferrali M, Signorini C, Sugherini L, Pompella A, Maura L, Caciotti B, Ciccoli L, Comporti M (1997) Release of free redoxactive iron in the liver and DNA oxidative damage following phenylhydarzine intoxication. Biochem Pharmacol 53(11):1743–1751PubMedCrossRef

Foroughi A, Zangeneh MM, Zangeneh A, Kazemi N (2016) A survey on antibacterial activities of Allium eriophyllum alcoholic extract: an ethnomedicinal plant. Iran J Public Health 45(2):32–32

Giffin H, Allen E (1993) The control and complete remission of polycythemia vera following the prolonged administration of phenylhydrazine hydrochloride. Am J Med Sci 185:1–13

Goldberg B, Stern A (1975) The generation of O2-by the interaction of the hemolytic agent, phenylhydrazine, with human hemoglobin. J Biol Chem 25(6):2401–2403

Goodarzi N, Zangeneh MM, Zangeneh A, Najafi F, Tahvilian R (2017) Protective effects of ethanolic extract of Allium Saralicum R.M. Fritsch on CCl₄- induced hepatotoxicity in mice. J Rafsanjan Univ Med Sci 16(3):227–238

Goodarzi N, Zangeneh MM, Zangeneh A (2018) The effect of ethanolic extract of Allium saralicum R.M. Fritsch on diabetic hepatopathy in male mice. Sci Res J Shahed Univ 25:21–30

Hamelian M, Zangeneh MM, Amisama A, Varmira K, Veisi H (2018) Green synthesis of silver nanoparticles using Thymus kotschyanus extract and evaluation of their antioxidant, antibacterial and cytotoxic effects. Appl Organometal Chem 32. https://doi.org/10.1002/aoc.4458

Han JH (2000) Antimicrobial food packaging. J Food Technol 54(3):56–65

Hochstein P (1971) Glucose – 6 – phosphate dehydrogenase deficiency: mechanism of drug – induced hemolysis. Exp Eye Res 11(3):389–395PubMedCrossRef

Holley RA, Patel D (2005) Improvement in shelf life and safety of perishable foods by plant essential oils and smoke antimicrobials. J Food Microbiol 22(4):273–292CrossRef

Horn S, Gopas J, Bashan N (1990) A lectin like receptor on murine macrophage is involved in the recognition and phagocytosis of human red cells oxidized by phenylhydrazine. Biochem Pharmacol 39(4):775–780PubMedCrossRef

Janahmadi Z, Nekooeian AA, Mozafari M (2015) Hydroalcoholic extract of Allium eriophyllum leaves attenuates cardiac impairment in rats with simultaneous type 2 diabetes and renal hypertension. Res Pharm Sci 10(2):125–133PubMedPubMedCentral

Kim J, Marshal M, Wei C (1995) Antibacterial activity of some essential oil components against five food borne pathogens. J Agric Food Chem 43(11):2839–2845CrossRef

Lee HW, Kim H, Ryuk JA, Kil KJ, Ko BS (2014) Hemopoietic effect of extracts from constituent herbal medicines of Samul-tang on phenylhydrazine-induced hemolytic anemia in rats. Int J Clin Exp Pathol 7(9):6179–6185PubMedPubMedCentral

Lippi G, Schena F, Salvagno GL, Aloe R, Banfi G, Guidi GC (2012) Foot-strike haemolysis after a 60-km ultramarathon. Blood Transfus 10(3):377–383PubMedPubMedCentral

Mansuy D, Battioni P, Mahy JP, Gillet G (1982) Comparison of the hemoglobin reactions with methyl-and phenyl-hydrazine; intermediate formation of a hemoglobin Fe-(II)-methyldiazene complex. Biochem Biophys Res Commun 106(1):30–36PubMedCrossRef

Mozafari M, Nekooeian AA, Janahmadi Z (2015) The antihypertensive effects of hydroalcoholic extract of allium eriophyllum in leaves in rats with simultaneous type 2 diabetes and renal hypertension. Int Cardiovasc Res J 9(1):34–40

Omenn GS, Goodman GE, Thornquist MD, Balmes J, Cullen MR, Glass A, Keogh JP, Meyskens FL, Valanis B, Williams JH, Barnhart S, Hammar S (1996) Effects of a combination of beta carotine and vitamin A on lung cancer and cardiovascular disease. N Engl J Med 334(18):1150–1155PubMedCrossRef

Pham-Quang-Chi (1979) Toxicity of inhaled phenylhydrazine (Russian). Gig Tr Prof Zabol 3:45–47

Qaseem A, Humphrey LL, Fitterman N, Starkey M, Shekelle P (2013) Clinical Guidelines Committee of the American College of Physicians. Treatment of anemia in patients with heart disease: a clinical practice guideline from the American College of Physicians. Ann Intern Med 159(11):770–779PubMedCrossRef

Rifkind RA (1965) Heinz body anaemia–an ultrastructural study II red cell sequestration and destruction. Blood 26(4):433–448PubMedCrossRef

Rifkind RA, Danon D (1965) Heinz body anaemia–an ultra structural study I Heinz body formation. Blood 25(6):885–895PubMedCrossRef

Sayyedrostami T, Pournaghi P, Ebrahimi Vosta-Kalaeea S, Zangeneh MM (2018) Evaluation of the wound healing activity of Chenopodium botrys leaves essential oil in rats (a short-term study). J Essent Oil Bear Plants 21(1):164–174CrossRef

Sherkatolabbasieh H, Hagh-Nazari L, Shafiezadeh S, Goodarzi N, Zangeneh MM, Zangeneh A (2017) Ameliorative effects of the ethanolic extract of Allium saralicum R.M. Fritsch on CCl₄-induced nephrotoxicity in mice: a stereological examination. Arch Biol Sci 69(3):535–543CrossRef

Telford RD, Sly GJ, Hahn AG, Cunningham RB, Bryant C, Smith JA (2003) Footstrike is the major cause of hemolysis during running. J Appl Physiol 94(1):38–42CrossRef

Witchett CE (1975) Exposure of dog erythrocytes in vivo to phenylhydrazine and monomethylhydrazine. A freeze-etch study of erythrocyte damage. Aerospace medical research laboratory, Aeorospace medical division, AFSC, Wright-Patterson Air Force Base, report no: AMRL-TR-74-88. NTIS Publication No AD-A011 555

Zhaleh M, Sohrabi N, Zangeneh MM, Zangeneh A, Moradi R, Zhaleh H (2018) Chemical composition and antibacterial effects of essential oil of Rhus coriaria fruits in the west of Iran (Kermanshah). J Essent Oil Bear Plants 21(2):493–501CrossRef

Title: Assessment of the anti-anemic effect of aqueous extract of Allium eriophyllum Boiss leaf in phenylhydrazine-treated Wistar male rats
Authors: Mohammad Mahdi Zangeneh
Mohsen Zhaleh
Saman Salmani
Akram Zangeneh
Maryam Almasi
Mohammad Reza Khedri
Khodabakhsh Rashidi
Publication date: 01-04-2019
Publisher: Springer London
Published in: Comparative Clinical Pathology / Issue 2/2019
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-018-2851-x

At a glance: The STEP trials

Springer Medicine

Assessment of the anti-anemic effect of aqueous extract of Allium eriophyllum Boiss leaf in phenylhydrazine-treated Wistar male rats

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 2/2019

Pathological and immunohistochemical evaluation of wound healing potential of Nigerian bee propolis in albino rats

Association between beta defensin gene polymorphism and clinical endometritis in dairy cows

Epidemiological profile and drug abuse: fatal occupational injuries in Espirito Santo, Brazil

Biochemical and physiological responses of Nile tilapia (Oreochromis niloticus Linn.) subjected to rapid increases of water temperature

Function indices of liver and kidney and haematological parameters of male Wistar rats after oral administration of aqueous extract of Terminalia avicennioides root barks

Medical management of conjunctivitis and shell rot in a red-eared slider (Trachemys scripta elegans)