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Journal of Cardiovascular Magnetic Resonance

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Open Access 01-12-2017 | Research

Assessment of local pulse wave velocity distribution in mice using k-t BLAST PC-CMR with semi-automatic area segmentation

Authors: Volker Herold, Stefan Herz, Patrick Winter, Fabian Tobias Gutjahr, Kristina Andelovic, Wolfgang Rudolf Bauer, Peter Michael Jakob

Published in: Journal of Cardiovascular Magnetic Resonance | Issue 1/2017

Abstract

Background

Local aortic pulse wave velocity (PWV) is a measure for vascular stiffness and has a predictive value for cardiovascular events. Ultra high field CMR scanners allow the quantification of local PWV in mice, however these systems are yet unable to monitor the distribution of local elasticities.

Methods

In the present study we provide a new accelerated method to quantify local aortic PWV in mice with phase-contrast cardiovascular magnetic resonance imaging (PC-CMR) at 17.6 T. Based on a k-t BLAST (Broad-use Linear Acquisition Speed-up Technique) undersampling scheme, total measurement time could be reduced by a factor of 6. The fast data acquisition enables to quantify the local PWV at several locations along the aortic blood vessel based on the evaluation of local temporal changes in blood flow and vessel cross sectional area. To speed up post processing and to eliminate operator bias, we introduce a new semi-automatic segmentation algorithm to quantify cross-sectional areas of the aortic vessel. The new methods were applied in 10 eight-month-old mice (4 C57BL/6J-mice and 6 ApoE ^(−/−)-mice) at 12 adjacent locations along the abdominal aorta.

Results

Accelerated data acquisition and semi-automatic post-processing delivered reliable measures for the local PWV, similiar to those obtained with full data sampling and manual segmentation. No statistically significant differences of the mean values could be detected for the different measurement approaches. Mean PWV values were elevated for the ApoE ^(−/−)-group compared to the C57BL/6J-group (3.5 ± 0.7 m/s vs. 2.2 ± 0.4 m/s, p < 0.01). A more heterogeneous PWV-distribution in the ApoE ^(−/−)-animals could be observed compared to the C57BL/6J-mice, representing the local character of lesion development in atherosclerosis.

Conclusion

In the present work, we showed that k-t BLAST PC-MRI enables the measurement of the local PWV distribution in the mouse aorta. The semi-automatic segmentation method based on PC-CMR data allowed rapid determination of local PWV. The findings of this study demonstrate the ability of the proposed methods to non-invasively quantify the spatial variations in local PWV along the aorta of ApoE ^(−/−)-mice as a relevant model of atherosclerosis.

Hansen TW, Staessen JA, Torp-Pedersen C, Rasmussen S, Thijs L, Ibsen H, Jeppesen J. Prognostic value of aortic pulse wave velocity as index of arterial stiffness in the general population. Circulation. 2006; 113:664–70.CrossRef

Mitchell GF, Hwang SJ, Vasan RS, Larson MG, Pencina MJ, Hamburg NM, Vita JA, Levy D, Benjamin EJ. Arterial stiffness and cardiovascular events the framingham heart study. Circulation. 2010; 121:505–11.CrossRefPubMedPubMedCentral

Cavalcante JL, Lima JAC, Redheuil A, Al-Mallah MH. Aortic stiffness: current understanding and future directions. J Am Coll Cardiol. 2011; 57:1511–22.CrossRefPubMed

Laurent S, Katsahian S, Fassot C, Tropeano AI, Gautier I, Laloux B, Boutouyrie P. Aortic stiffness is an independent predictor of fatal stroke in essential hypertension. Stroke. 2003; 34:1203–6.CrossRefPubMed

Sutton-Tyrrell K, Najjar SS, Boudreau RM, Venkitachalam L, Kupelian V, Simonsick EM, Havlik R, Lakatta EG, Spurgeon H, Kritchevsky S, et al.Elevated aortic pulse wave velocity, a marker of arterial stiffness, predicts cardiovascular events in well-functioning older adults. Circulation. 2005; 111:3384–90.CrossRefPubMed

Pannier B, Guérin AP, Marchais SJ, Safar ME, London GM. Stiffness of capacitive and conduit arteries prognostic significance for end-stage renal disease patients. Hypertension. 2005; 45:592–6.CrossRefPubMed

Asmar R, Benetos A, Topouchian J, Laurent P, Pannier B, Brisac AM, Target R, Levy BI. Assessment of arterial distensibility by automatic pulse wave velocity measurement validation and clinical application studies. Hypertension. 1995; 26:485–90.CrossRefPubMed

Cruickshank K, Riste L, Anderson SG, Wright JS, Dunn G, Gosling RG. Aortic pulse-wave velocity and its relationship to mortality in diabetes and glucose intolerance an integrated index of vascular function?Circulation. 2002; 106:2085–90.CrossRefPubMed

Redheuil A, Yu WC, Wu CO, Mousseaux E, de Cesare A, Yan R, Kachenoura N, Bluemke D, Lima JA. Reduced ascending aortic strain and distensibility earliest manifestations of vascular aging in humans. Hypertension. 2010; 55:319–26.CrossRefPubMedPubMedCentral

10.

Vulliémoz S, Stergiopulos N, Meuli R. Estimation of local aortic elastic properties with mri. Magn Reson Med. 2002; 47:649–54.CrossRefPubMed

11.

Meir KS, Leitersdorf E. Atherosclerosis in the apolipoprotein e–deficient mouse a decade of progress. Arterioscler Throm Vas. 2004; 24:1006–14.CrossRef

12.

Plump AS, Breslow JL. Apolipoprotein e and the apolipoprotein e-deficient mouse. Annu Rev Nutr. 1995; 15:495–518.CrossRefPubMed

13.

Chatterjee S, Bedja D, Mishra S, Amuzie C, Avolio A, Kass DA, Berkowitz D, Renehan M. Inhibition of glycosphingolipid synthesis ameliorates atherosclerosis and arterial stiffness in apolipoprotein e-/- mice and rabbits fed a high-fat and-cholesterol diet. Circulation. 2014; 129:2403–13.CrossRefPubMedPubMedCentral

14.

Hartley CJ, Reddy AK, Madala S, Martin-McNulty B, Vergona R, Sullivan ME, Halks-Miller M, Taffet GE, Michael LH, Entman ML, Wang YX. Hemodynamic changes in apolipoprotein e-knockout mice. Am J Physiol Heart Circ Physiol. 2000; 279:H2326–34.PubMed

15.

Parczyk M, Herold V, Klug G, Bauer WR, Rommel E, Jakob PM. Regional in vivo transit time measurements of aortic pulse wave velocity in mice with high-field cmr at 17.6 tesla. J Cardiovasc Magn Reson. 2010; 12:72.CrossRefPubMedPubMedCentral

16.

Zhao X, Pratt R, Wansapura J. Quantification of aortic compliance in mice using radial phase contrast mri. J Magn Reson Imaging. 2009; 30:286–91.CrossRefPubMed

17.

Laurent S, Cockcroft J, Van Bortel L, Boutouyrie P, Giannattasio C, Hayoz D, Pannier B, Vlachopoulos C, Wilkinson I, Struijker-Boudier H. European Network for Non-invasive Investigation of Large Arteries. Expert consensus document on arterial stiffness: methodological issues and clinical applications. Eur Heart J. 2006; 27:2588–605.CrossRefPubMed

18.

Assemat P, Siu K, Armitage J, Hokke S, Dart A, Chin-Dusting J, Hourigan K. Haemodynamical stress in mouse aortic arch with atherosclerotic plaques: Preliminary study of plaque progression. Comput Struct Biotechnol J. 2014; 10:98–106.CrossRefPubMedPubMedCentral

19.

Ben-Dor I, Barbash IM, Aly O, Dvir D, Deksissa T, Okubagzi P, Torguson R, Lindsay J, Satler LF, Pichard AD, et al.Correlation of brain natriuretic peptide levels in patients with severe aortic stenosis undergoing operative valve replacement or percutaneous transcatheter intervention with clinical, echocardiographic, and hemodynamic factors and prognosis. Am J Cardiol. 2013; 112:574–9.CrossRefPubMed

20.

Gotschy A, Bauer E, Schrodt C, Lykowsky G, Ye YX, Rommel E, Jakob PM, Bauer WR, Herold V. Local arterial stiffening assessed by mri precedes atherosclerotic plaque formation. Circ Cardiovasc Imaging. 2013; 6:916–23.CrossRefPubMed

21.

Herold V, Parczyk M, Mörchel P, Ziener CH, Klug G, Bauer WR, Rommel E, Jakob PM. In vivo measurement of local aortic pulse-wave velocity in mice with mr microscopy at 17.6 tesla. Magn Reson Med. 2009; 61:1293–9.CrossRefPubMed

22.

Winter P, Kampf T, Helluy X, Gutjahr FT, Meyer CB, Rommel E, Bauer WR, Jakob PM, Herold V. Fast retrospectively triggered local pulse-wave velocity measurements in mice with cmr-microscopy using a radial trajectory. J Cardiovasc Magn Reson. 2013; 15:88–98.CrossRefPubMedPubMedCentral

23.

Tsao J, Boesiger P, Pruessmann KP. k-t blast and k-t sense: dynamic mri with high frame rate exploiting spatiotemporal correlations. Magn Reson Med. 2003; 50:1031–42.CrossRefPubMed

24.

Kozerke S, Tsao J. Reduced data acquisition methods in cardiac imaging. Top Magn Reson Imaging. 2004; 15:161–8.CrossRefPubMed

25.

Stadlbauer A, van der Riet W, Crelier G, Salomonowitz E. Accelerated time-resolved three-dimensional MR velocity mapping of blood flow patterns in the aorta using SENSE and k-t BLAST. Eur J Radiol. 2010; 75:e15–21.CrossRefPubMed

26.

Gotschy A, Bauer WR, Winter P, Nordbeck P, Rommel E, Jakob PM, Herold V. Local versus global aortic pulse wave velocity in early atherosclerosis: An animal study in ApoE-/–mice using ultrahigh field MRI. PLoS ONE. 2017; 12:e0171603.CrossRefPubMedPubMedCentral

27.

Wang YX. Cardiovascular functional phenotypes and pharmacological responses in apolipoprotein e deficient mice. Neurobiol Aging. 2005; 26:309–16.CrossRefPubMed

28.

Feintuch A, Ruengsakulrach P, Lin A, Zhang J, Zhou YQ, Bishop J, Davidson L, Courtman D, Foster FS, Steinman DA, Henkelman RM, Ethier CR. Hemodynamics in the mouse aortic arch as assessed by mri, ultrasound, and numerical modeling. Am J Physiol Heart Circ Physiol. 2007; 292:H884–92.CrossRefPubMed

29.

Nichols WW, ORourke MF. McDonalds blood flow in arteries. Theoretical, Experimental and Clinical Principles. 5th ed. Oxford: University Press; 2005.

30.

Latham RD, Westerhof N, Sipkema P, Rubal BJ, Reuderink P, Murgo JP. Regional wave travel and reflections along the human aorta: a study with six simultaneous micromanometric pressures. Circulation. 1985; 72:1257–69.CrossRefPubMed

Title: Assessment of local pulse wave velocity distribution in mice using k-t BLAST PC-CMR with semi-automatic area segmentation
Authors: Volker Herold
Stefan Herz
Patrick Winter
Fabian Tobias Gutjahr
Kristina Andelovic
Wolfgang Rudolf Bauer
Peter Michael Jakob
Publication date: 01-12-2017
Publisher: BioMed Central
Published in: Journal of Cardiovascular Magnetic Resonance / Issue 1/2017
Electronic ISSN: 1532-429X
DOI: https://doi.org/10.1186/s12968-017-0382-2

At a glance: The STEP trials

Springer Medicine

Assessment of local pulse wave velocity distribution in mice using k-t BLAST PC-CMR with semi-automatic area segmentation

Abstract

Background

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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