Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The ONWARDS insulin icodec trials

A round-up of the ONWARDS phase 3 clinical trials evaluating the novel weekly insulin icodec in people with diabetes.
ADVANCED SEARCH
Log in
Top
Diagnostic Pathology
Published in: Diagnostic Pathology 1/2014

Open Access 01-12-2014 | Research

Assessment of IgG antibodies to Pseudomonas aeruginosa in patients with cystic fibrosis by an enzyme-linked immunosorbent assay (ELISA)

Authors: Renan Marrichi Mauch, Cláudio Lúcio Rossi, José Dirceu Ribeiro, Antínio Fernando Ribeiro, Marcos Tadeu Nolasco da Silva, Carlos Emílio Levy

Published in: Diagnostic Pathology | Issue 1/2014

Login to get access

Abstract

Background

The usefulness of serological tests for detection of P. aeruginosa pulmonary infection in cystic fibrosis (CF) is controversial. Here, we assessed the value of detecting anti-P. aeruginosa IgG by a quantitative enzyme-linked immunosorbent assay (ELISA) for identification of P. aeruginosa infection in patients with cystic fibrosis.

Methods

Serum concentrations of anti-P. aeruginosa IgG were assessed in 117 CF patients classified according to their P. aeruginosa colonization/infection status (never colonized; free of infection; intermittently colonized and chronically infected) and in 53 healthy subjects by the ELISA test standardized with the St-Ag:1-17 antigen.

Results

The rate of IgG seropositivity and the median of IgG concentrations of this antibody in patients chronically infected were significantly higher than those found in the other CF groups and in the healthy control group.

Conclusion

Detection of anti-P. aeruginosa IgG can be an useful tool for identification of P. aeruginosa chronic infection in patients with CF.

Virtual Slides

The virtual slide(s) for this article can be found here: http://​www.​diagnosticpathol​ogy.​diagnomx.​eu/​vs/​13000_​2014_​158
Appendix
Available only for authorised users
Literature
1.
Gonçalves AC, Marson FAL, Mendonça RMH, Ribeiro JD, Ribeiro AF, Paschoal IA, Levy CE: Saliva as a potential tool for cystic fibrosis diagnosis. Diagn Pathol. 2013, 8: 46-10.1186/1746-1596-8-46.PubMedPubMedCentralCrossRef
2.
Kiska DL, Riddell SW: Practical laboratory aspects of Cystic Fibrosis microbiology: an Update Part I. Clin Microbiol Newslett. 2012, 34 (Suppl 4): 27-31. 10.1016/j.clinmicnews.2012.01.003.CrossRef
3.
Ginori A, Barone A, Bennett D, Butorano MAGM, Mastrougiulio MG, Fossi A, Rottoli P, Spina D: Diffuse panbronchiolitis in a patient with common variable immunodeficiency: a casual association or a pathogenic correlation?. Diagn Pathol. 2014, 9: 12-10.1186/1746-1596-9-12.PubMedPubMedCentralCrossRef
4.
Goldman T, Kähler D, Schultz H, Abdullah M, Lang DS, Stellmacher F, Vollmer E: On the significance of Surfactant Protein-A within the human lungs. Diagn Pathol. 2009, 4: 8-10.1186/1746-1596-4-8.CrossRef
5.
Nagata N, Kitasato Y, Wakamatsu K, Kawabata M, Fukushima K, Kajiki A, Kitahara Y, Watanabe K: Prognostic value of immunohistochemical surfactant protein A expression in regenerative/hyperplasic alveolar epithelial cells in idiopathic intersticial pneumonias. Diagn Pathol. 2011, 6: 25-10.1186/1746-1596-6-25.PubMedPubMedCentralCrossRef
6.
Hammami S, Boutiba-Ben Boubaker I, Ghozzi R, Saidani M, Amine S, Ben RS: Nosocomial outbreak of imipenem-resistant Pseudomonas aeruginosa producing VIM-2 metallo-β-lactamase in a kidney transplantation unit. Diagn Pathol. 2011, 6: 106-10.1186/1746-1596-6-106.PubMedPubMedCentralCrossRef
7.
Gilligan PH, Kiska DL, Appleman MD: Cystic fibrosis microbiology. Cumulative techniques and procedures in clinical microbiology. Edited by: Appleman MD. 2006, ASM Press, Washington, DC, 1-36.
8.
Lee TWR, Brownlee KG, Conway SP, Denton M, Littlewood JM: Evaluation of a definition for chronic Pseudomonas aeruginosa infection in cystic fibrosis patients. J Cyst Fibros. 2003, 2 (Suppl 1): 29-34. 10.1016/S1569-1993(02)00141-8.PubMedCrossRef
9.
Taccetti G, Bianchini E, Cariani L, Buzzetti R, Constantini D, Trevisan F, Zavataro L, Campana S: Early antibiotic treatment for Pseudomonas aeruginosa eradication in patients with Cystic Fibrosis: a randomized multicenter study comparing two protocols. Thorax. 2012, 67: 853-859. 10.1136/thoraxjnl-2011-200832.PubMedCrossRef
10.
Tramper-Standers GA, van der Ent CK, Wolfs TFW: Detection of Pseudomonas aeruginosa in patients with cystic fibrosis. J Cyst Fibros. 2005, 4: 37-43. 10.1016/j.jcf.2005.05.009.CrossRef
11.
12.
Rosenfeld M, Emerson J, Accurso F, Armstrong D, Castile R, Grinwood K, Hiatt P, McCoy K, McNamara S, Ramsey B, Wagener J: Diagnostic accuracy of oropharyngeal cultures in infants and young children with cystic fibrosis. Pediatr Pulmonol. 1999, 28: 321-328. 10.1002/(SICI)1099-0496(199911)28:5<321::AID-PPUL3>3.0.CO;2-V.PubMedCrossRef
13.
Burns JL, Rolain JM: Culture-based diagnostic microbiology in cystic fibrosis: Can we simplify the complexity?. J Cyst Fibros. 2014, 13: 1-9. 10.1016/j.jcf.2013.09.004.PubMedCrossRef
14.
Goddard AF, Staudinger BJ, Dowd SE, Joshi-Datar A, Wolcott RD, Aitken ML, Fligner CL, Singh PK: Direct sampling of cystic fibrosis lungs indicates that DNA-based analyses of upper-airway specimens can misrepresent lung microbiota. Proc Natl Acad Sci U S A. 2012, 109: 13769-13774. 10.1073/pnas.1107435109.PubMedPubMedCentralCrossRef
15.
Da Silva Filho LVF, Tateno AF, Martins KM, Azzuz Chernishev AC, Garcia DO, Haug M, Meisner C, Rodrigues JC, Döring G: The combination of PCR and serology increases the diagnosis of Pseudomonas aeruginosa colonization/infection in cystic fibrosis. Pediatr Pulmonol. 2007, 42 (Suppl 10): 938-944. 10.1002/ppul.20686.PubMedCrossRef
16.
Pressler T, Frederiksen B, Skov M, Garred P, Koch C, Høiby N: Early rise of anti-Pseudomonas antibodies and a mucoid phenotype of Pseudomonas aeruginosa are risk factors for development of chronic lung infection- A case control study. J Cyst Fibros. 2006, 5: 9-15. 10.1016/j.jcf.2005.11.002.PubMedCrossRef
17.
Pressler T, Karpati F, Granström M, Knudsen PK, Lindbald A, Hjelte L, Olesen HV, Meyer P, Høiby N: Scandinavian CF Study Consortium. Diagnostic significance of mesuraments of specific IgG antibodies to Pseudomonas aeruginosa by three different serological methods. J Cyst Fibros. 2009, 8: 37-42. 10.1016/j.jcf.2008.08.002.PubMedCrossRef
18.
Mauch RM, Levy CE: Serum antibodies toPseudomonas aeruginosain cystic fibrosis: a systematic review.J Cyst Fibros . in press.,
19.
De Boeck K, Derichs N, Fajac I, de Jonge HR, Bronsveld I, Sermet I, Vermeulen F, Sheppard DN, Cuppens H, Hug M, Melotti P, Middleton PG, Wilschanski M: New clinical diagnostic procedures for cystic fibrosis in Europe. J Cyst Fibros. 2011, 10 (Suppl 2): s53-s66. 10.1016/S1569-1993(11)60009-X.PubMedCrossRef
20.
Farrell PM, Rosenstein BJ, White TB, Accurso FJ, Castellani C, Cutting GR, Durie PR, Legrys VA, Massie J, Parad RB, Rock MJ, Campbell PW: Guidelines for Diagnosis of Cystic Fibrosis in Newborns through Older Adults: Cystic Fibrosis Foundation Consensus Report. J Pediatr. 2008, 153 (Suppl 3): s4-s14. 10.1016/j.jpeds.2008.05.005.PubMedPubMedCentralCrossRef
21.
Ooi CY, Dupuis A, Elis L, Jarvi K: Comparing the American and European diagnostic guidelines for cystic fibrosis: same disease, different language?. Thorax. 2012, 67: 618-624. 10.1136/thoraxjnl-2011-201454.PubMedCrossRef
22.
Høiby N, Flensborg EW, Beck B, Friis B, Jacobsen SV, Jacobsen L:Pseudomonas aeruginosa in cystic fibrosis. Diagnostic and prognostic significance of Pseudomonas aeruginosa precipitins determined by means of crossed immunoelectrophoresis. Scand J Respir Dis. 1977, 58: 65-79.PubMed
23.
Doring G, Høiby N: Longitudinal study of immune response to P. aeruginosa antigens in cystic fibrosis. Infect Immun. 1983, 42: 197-201.PubMedPubMedCentral
24.
Hancock K, Tsang VCW: Development and optimization of the FAST-ELISA for detecting antibodies to Schistosoma mansoni. J Immunol Methods. 1986, 92: 167-176. 10.1016/0022-1759(86)90162-6.PubMedCrossRef
25.
Maddison SE: The present status of serodiagnosis and seroepidemiology of schistosomiasis. Diagn Microbiol Infect Dis. 1987, 7: 93-105. 10.1016/0732-8893(87)90026-5.PubMedCrossRef
26.
Proesmans M, Balinska-Mizkiewicz W, Dupont L, Bossuyt X, Verhaegen J, Høiby N, de Boeck K: Evaluating the "Leeds criteria" for Pseudomonas aeruginosa infection in a cystic fibrosis centre. Eur Respir J. 2006, 27: 937-943.PubMed
27.
Anstead M, Heltshe S, Khan U, Barbieri JT, Langkamp M, Döring G, Dharia S, Gibson RL, Treggiari MM, Lymp J, Rosenfeld M, Ramsey B:Pseudomonas aeruginosa serology and risk for re-isolation in the EPIC trial. J Cyst Fibros. 2012, 12 (Suppl 2): 147-153.PubMedPubMedCentral
28.
Brett MM, Ghoneim ATM, Littlewood JM: Serum antibodies to Pseudomonas aeruginosa in cystic fibrosis. Arch Dis Child. 1986, 61: 1114-1120. 10.1136/adc.61.11.1114.PubMedPubMedCentralCrossRef
29.
Johansen HK, Norregaard L, Gotzsche PC, Pressler T, Koch C, Høiby N: Antibody response to Pseudomonas aeruginosa in cystic fibrosis patients: a marker of therapeutic success? A 30-year cohort study of survival in danish of patients after onset of chronic Pseudomonas aeruginosa lung infection. Pediatr Pulmonol. 2004, 37: 427-432. 10.1002/ppul.10457.PubMedCrossRef
30.
Ratjen F, Walter H, Haug M, Meisner MC, Grasemann H, Döring G: Diagnostic value of serum antibodies in early Pseudomonas aeruginosa infection in cystic fibrosis patients. Pediatr Pulmonol. 2007, 42: 349-355. 10.1002/ppul.20562.CrossRef
31.
Kappler M, Kraxner A, Reinhardt D, Ganster B, Griese M, Lang T: Diagnostic and prognostic value of serum antibodies against Pseudomonas aeruginosa in cystic fibrosis. Thorax. 2006, 61: 684-688. 10.1136/thx.2005.049536.PubMedPubMedCentralCrossRef
32.
Tramper-Standers GA, van der Ent CK, Slieker MG, Terheggen-Lagro SWJ, van Berkhout FT, Kimpen JLL, Wolfs TFW: Diagnostic value of serological tests against Pseudomonas aeruginosa in a large cystic fibrosis population. Thorax. 2006, 61: 689-693. 10.1136/thx.2005.054726.CrossRef
33.
Pedersen S, Espersen F, Høiby N: Diagnosis for chronic Pseudomonas aeruginosa infection in cystic fibrosis by enzinme-linked immunosorbent assay. J Clin Microbiol. 1987, 25 (Suppl 10): 1830-1836.PubMedPubMedCentral
34.
Burns JL, Gibson RL, McNamara S, Yim D, Emerson J, Rosenfeld M, Hiatt P, McCoy K, Castile R, Smith AL, Ramsey BW: Longitudinal assessment of Pseudomonas aeruginosa in young children with cystic fibrosis. J Infect Dis. 2001, 183: 444-452. 10.1086/318075.PubMedCrossRef
35.
Pressler T, Bohmova C, Conway S, Dumcius S, Hjelte L, Høiby N, Kollberg H, Tümmler B, Vavrova V: Chronic Pseudomonas aeruginosa infection definition: EuroCareCF Working Group report. J Cyst Fibros. 2011, 10 (Suppl 2): S75-S78. 10.1016/S1569-1993(11)60011-8.PubMedCrossRef
36.
Metadata
Title
Assessment of IgG antibodies to Pseudomonas aeruginosa in patients with cystic fibrosis by an enzyme-linked immunosorbent assay (ELISA)
Authors
Renan Marrichi Mauch
Cláudio Lúcio Rossi
José Dirceu Ribeiro
Antínio Fernando Ribeiro
Marcos Tadeu Nolasco da Silva
Carlos Emílio Levy
Publication date
01-12-2014
Publisher
BioMed Central
Published in
Diagnostic Pathology / Issue 1/2014
Electronic ISSN: 1746-1596
DOI
https://doi.org/10.1186/s13000-014-0158-z

Other articles of this Issue 1/2014

Diagnostic Pathology 1/2014 Go to the issue

Research

Whole DNA methylome profiling in lung cancer cells before and after epithelial-to-mesenchymal transition

Research

Amplification of Mdmx and overexpression of MDM2 contribute to mammary carcinogenesis by substituting for p53 mutations

Research

Automated quantification of Ki-67 proliferative index of excised neuroendocrine tumors of the lung

Research

Heterogenous mismatch-repair status in colorectal cancer

Research

Wogonin modulates hydroperoxide-induced apoptosis via PI3K/Akt pathway in retinal pigment epithelium cells

Research

Modulation of insulin/IGFs pathways by sirtuin-7 inhibition in drug-induced chemoreistance