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01-05-2013 | Technical Note

Assessment of application value of 19 autosomal short tandem repeat loci of GoldenEye^TM 20A kit in forensic paternity testing

Authors: Yan-Mei Huang, Jie Wang, Zhangping Jiao, Liu Yang, Xinning Zhang, Hui Tang, Yacheng Liu

Published in: International Journal of Legal Medicine | Issue 3/2013

Abstract

This study was carried out to assess the application value of 19 autosomal short tandem repeat (STR) loci of GoldenEye^TM 20A kit, in which 13 combined DNA index system core STR loci and PentaE, PentaD, D2S1338, D19S433, D12S391, and D6S1043 of six STR loci could be used in forensic paternity testing in Chinese population. We amplified the genomic DNA from blood samples on FTA paper of 289 paternity testing cases by using the GoldenEye^TM 20A kit. The amplified products were detected by capillary electrophoresis, and then the genotypes of 20 genetic markers including 19 STR loci as well as Amelogenin for sex determination were analyzed by GeneMapper v3.2 and GeneMarker HID Software. The results of genotypes were compared to the three commonly used commercial kits including AmpFℓSTR Identifiler^TM, PowerPlex^TM16, and AmpFℓSTR Sinofiler^TM kits. Compared to the three other common commercial kits, the GoldenEye^TM 20A kit had higher value of combined paternity index in certainty of paternity or non-exclusion paternity cases, and more numbers of STR loci were excluded in exclusionary paternity cases. Our data in this study showed that the GoldenEye^TM 20A kit has a higher application value in forensic paternity testing and will be of help for kinship analysis.

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Westen AA, Haned H, Grol LJ, Harteveld J, van der Gaag KJ, de Knijff P, Sijen T (2012) Combining results of forensic STR kits: HDplex validation including allelic association and linkage testing with NGM and Identifiler loci. Int J Legal Med 126(5):781–9. doi:10.1007/s00414-012-0724-4 PubMedCrossRef

Collins PJ, Hennessy LK, Leibelt CS, Roby RK, Reeder DJ, Foxall PA (2004) Developmental validation of a single-tube amplification of the 13 CODIS STR loci, D2S1338, D19S433, and amelogenin: the AmpFlSTR Identifiler PCR Amplification Kit. J Forensic Sci 49(6):1265–1277PubMedCrossRef

Krenke BE, Tereba A, Anderson SJ, Buel E, Culhane S, Finis CJ, Tomsey CS, Zachetti JM, Masibay A, Rabbach DR, Amiott EA, Sprecher CJ (2002) Validation of a 16-locus fluorescent multiplex system. J Forensic Sci 47(4):773–785PubMed

Li L, Ge J, Zhang S, Guo J, Zhao S, Li C, Tang H, Davis C, Budowle B, Hou Y, Liu Y (2012) Maternity exclusion with a very high autosomal STRs kinship index. Int J Legal Med 126(4):645–648PubMedCrossRef

Nothnagel M, Schmidtke J, Krawczak M (2010) Potentials and limits of pairwise kinship analysis using autosomal short tandem repeat loci. Int J Legal Med 124(3):205–215PubMedCrossRef

Phillips C, Fondevila M, Garcia-Magarinos M, Rodriguez A, Salas A, Carracedo A, Lareu MV (2008) Resolving relationship tests that show ambiguous STR results using autosomal SNPs as supplementary markers. Forensic Sci Int Genet 2(3):198–204PubMedCrossRef

Wang J, Huang YM, Zhang QX, Wang J, Tang H, Jiao ZP, Liu YC (2012) The developmental validation of the homemade GoldeneyeTM20A PCR amplification kit. Chin J Forensic Med 27(1):12–15

Witt S, Neumann J, Zierdt H, Gebel G, Roscheisen C (2012) Establishing a novel automated magnetic bead-based method for the extraction of DNA from a variety of forensic samples. Forensic Sci Int Genet 6(5):539–47. doi:10.1016/j.fsigen.2012.01.00 PubMedCrossRef

Hwa HL, Chang YY, Lee JC, Yin HY, Tseng LH, Su YN, Ko TM (2011) Fourteen non-CODIS autosomal short tandem repeat loci multiplex data from Taiwanese. Int J Legal Med 125(2):219–226PubMedCrossRef

10.

Pu CE, Linacre A (2007) CPI distribution and cutoff values for duo kinship testing. Chin J Physiol 50(5):232–239PubMed

11.

Bar W, Brinkmann B, Budowle B, Carracedo A, Gill P, Lincoln P, Mayr W, Olaisen B (1997) DNA recommendations. Further report of the DNA Commission of the ISFH regarding the use of short tandem repeat systems. International Society for Forensic Haemogenetics. Int J Legal Med 110(4):175–176PubMedCrossRef

12.

Carracedo A, Butler JM, Gusmao L, Parson W, Roewer L, Schneider PM (2010) Publication of population data for forensic purposes. Forensic Sci Int Genet 4(3):145–147PubMedCrossRef

13.

Butler JM (2007) Short tandem repeat typing technologies used in human identity testing. Biotechniques 43(4):ii–vPubMedCrossRef

14.

Gjertson DW, Brenner CH, Baur MP, Carracedo A, Guidet F, Luque JA, Lessig R, Mayr WR, Pascali VL, Prinz M, Schneider PM, Morling N (2007) ISFG: recommendations on biostatistics in paternity testing. Forensic Sci Int Genet 1(3–4):223–231PubMedCrossRef

15.

Morling N, Allen RW, Carracedo A, Geada H, Guidet F, Hallenberg C, Martin W, Mayr WR, Olaisen B, Pascali VL, Schneider PM (2002) Paternity Testing Commission of the International Society of Forensic Genetics: recommendations on genetic investigations in paternity cases. Forensic Sci Int 129(3):148–157PubMedCrossRef

16.

Wu XY, Yang QE, Liu YC, Lu HL, Li SB, Li L, Liu C, Wu WW, Sun HY, Zhu YL, Xu BY, Lu D (2010) Establishment of standard and conclusion expression of paternity testing. J Sun Yat-Sen University (Medical Sciences) 31(1):20–22,44

17.

Zhu YL, Huang YM, Wu XY (2006) How to draw a conclusion in motherless parentage testing using short tandem repeats as genetic makers. Fa Yi Xue Za Zhi 22(4):281–284PubMed

18.

Lu D, Liu Q, Wu W, Zhao H (2012) Mutation analysis of 24 short tandem repeats in Chinese Han population. Int J Legal Med 126(2):331–335PubMedCrossRef

19.

Zidkova A, Horinek A, Kebrdlova V, Korabecna M (2011) Application of the new insertion-deletion polymorphism kit for forensic identification and parentage testing on the Czech population. Int J Legal Med 127(1):7–10. doi:10.1007/s00414-011-0649-3 PubMedCrossRef

20.

Thomson JA, Pilotti V, Stevens P, Ayres KL, Debenham PG (1999) Validation of short tandem repeat analysis for the investigation of cases of disputed paternity. Forensic Sci Int 100(1–2):1–16PubMedCrossRef

21.

Brinkmann B, Klintschar M, Neuhuber F, Huhne J, Rolf B (1998) Mutation rate in human microsatellites: influence of the structure and length of the tandem repeat. Am J Hum Genet 62(6):1408–1415PubMedCrossRef

Title: Assessment of application value of 19 autosomal short tandem repeat loci of GoldenEyeTM 20A kit in forensic paternity testing
Authors: Yan-Mei Huang
Jie Wang
Zhangping Jiao
Liu Yang
Xinning Zhang
Hui Tang
Yacheng Liu
Publication date: 01-05-2013
Publisher: Springer-Verlag
Published in: International Journal of Legal Medicine / Issue 3/2013
Print ISSN: 0937-9827
Electronic ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-013-0842-7

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Assessment of application value of 19 autosomal short tandem repeat loci of GoldenEye^TM 20A kit in forensic paternity testing

Abstract

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Abstract

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